1. Semen characteristics and sperm DNA fragmentation in infertile men with low and high levels of seminal reactive oxygen species.
- Author
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Mahfouz R, Sharma R, Thiyagarajan A, Kale V, Gupta S, Sabanegh E, and Agarwal A
- Subjects
- Adult, Antioxidants metabolism, Humans, In Situ Nick-End Labeling, Male, Middle Aged, Oxidative Stress physiology, Reactive Oxygen Species analysis, Retrospective Studies, DNA Fragmentation, Infertility, Male metabolism, Reactive Oxygen Species metabolism, Semen Analysis, Spermatozoa metabolism
- Abstract
Objective: To examine sperm motility, total antioxidant level (TAC), DNA fragmentation, and medical history in infertile men with high seminal high reactive oxygen species (ROS)., Design: Prospective study., Setting(s): Tertiary care hospital., Patient(s): Infertile men (n=101)., Intervention(s): Group I (n=57) included men with seminal ROS (<250 relative light units/sec/×10(6) sperm) while group II (n=44) included men with seminal ROS levels (≥250 relative light units/sec/×10(6) sperm)., Main Outcome Measure(s): Seminal ROS, TAC, sperm DNA fragmentation, ROS/TAC score were measured., Result(s): Group II had a high incidence of sperm DNA fragmentation than group I. The odds ratio of 1.25 for elevated ROS levels corresponded to >10% greater DNA fragmentation in our patients (95% confidence interval 1.01-1.53). Group II showed poor motility, a higher incidence of leukocytospermia, and higher ROS-TAC scores compared with group I. ROS was negatively correlated with sperm curvilinear velocity (r=-.24), linearity (r=-.24), and sperm motility (r=-.31). Sperm motility was correlated with %TUNEL(+ve) sperm (r=-.39)., Conclusion(s): An increase in seminal ROS levels by 25% was associated with a 10% increase in sperm DNA fragmentation. Sperm motility was affected by seminal ROS and sperm DNA fragmentation., (Copyright © 2010 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.)
- Published
- 2010
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