Aflatoxin M1 (AFM1) is a toxic and carcinogenic metabolite secreted in milk of dairy animals which have consumed the aflatoxin B1 contaminated feedstuff. In this study, 230 different types of milk samples comprising raw, pasteurized and ultra-high temperature treated milk were collected from three different geographical sites of Punjab during different seasons and analysed for the presence of AFM1 by using ELISA as screening, and HPLC with fluorescence detection as confirmatory method. Of total, 51% samples were detected positive for AFM1, while 45% and 38% samples were found with AFM1 levels higher than the tolerance limits established by the European commission and Food safety and standard authority of India respectively. Results of study indicated a high incidence and levels of AFM1 in different types of milk during all seasons. Owing to this, there is a potential risk of liver cancer in people of Punjab, India especially children due to milk consumption. Therefore, strict regulatory actions are urgently required to control AFM1 level in milk below the legal standard limits. There is need to initiate routine examination of dairy animal feeds for aflatoxins to reduce animal and consequently human exposure. [ABSTRACT FROM AUTHOR]