1. [Role of TLR4/NF-κB pathway for early change of synovial membrane in knee osteoarthritis rats]
- Author
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Xue-Zong, Wang, Dao-Fang, Ding, Yan, Xue, Xin-Feng, Gu, Jian, Pang, Min, Zhang, Yu-Xin, Zheng, Yue-Long, Cao, and Hong-Sheng, Zhan
- Subjects
Male ,Rats, Sprague-Dawley ,Toll-Like Receptor 4 ,Synovial Membrane ,NF-kappa B ,Animals ,Osteoarthritis, Knee ,Rats ,Signal Transduction - Abstract
To study role of TLR4/NF-κB pathway for early change of synovial membrane in knee osteoarthritis rats.Eighteen male SD rats weighted (200±20) g were randomly divided into 2 groups, namely control and model group, and 9 in each group. Knee OA model group was established by using modified Hulth method in model group. Control group was not treated. Synovial tissue and serum was extracted at 4 and 21 d after operation. Expression of CD14, TLR4, IL-1β, TNF-α, ADAMTS-4, MMP-13 were detected by real-time PCR respectively. NF-κB p65 protein was detected by Western-blot; serum concentrations of haluronic acid (HA), N-propeptide of type III procollagen(PIIINP) was detected by Elisa.Expression of CD14, ADAMTS-4, and NF-κB p65 in model group were higher than that of control group at 4 and 21 days after operation, while expression of TLR4, IL-1β, TNF-α and MMP-13 were higher than that of control group at 21 days after operation(NF-κB pathway could mediate occurrence of KOA by early activating and triggeringg synovial increasingly secreting inflammatory secretion CD14, TLR4, IL-1β, TNF-α, ADAMTS-4, MMP-13, PIIINP and HA.
- Published
- 2018