1. Impact of various factors on radioactivity distribution in different DBS papers.
- Author
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Ren X, Paehler T, Zimmer M, Guo Z, Zane P, and Emmons GT
- Subjects
- Adult, Blood Coagulation, Chemical Phenomena, Desiccation, Hematocrit, Humans, Humidity, Laboratories, Male, Temperature, Blood Chemical Analysis methods, Blood Specimen Collection instrumentation, Paper, Radioactivity
- Abstract
Background: Dried blood spot (DBS) sampling could potentially become the preferred blood collection technique in toxicological and clinical studies. Autoradiography was performed to study compound distribution within a dbs under different conditions using five papers, 31ETF, Grade 226, 903(®), FTA(®) and FTA(®) Elute., Results: The results showed an uneven distribution in all papers with common distribution patterns regardless of compounds: decreased concentrations along the edge, the volcano effect in the middle and the speckle pattern in the center. Treated papers were more readily influenced by environmental factors., Conclusion: Autoradiography enables visualization of a compound's distribution and can guide bioanalytical assay development by allowing convenient evaluation of factors, such as choice of paper, spotting volume, punch size, punch location, temperature and humidity.
- Published
- 2010
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