1. Using a quick dye for the diagnosis of microsporidiosis.
- Author
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Tovar, Luis Edgar Rodríguez, Ramírez, Heidi Giselle Rodríguez, Nava, Yazel Valdez, Garza, Alicia Magdalena Nevárez, and Romero, Rafael Ramírez
- Subjects
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MICROSPORIDIOSIS , *DYES & dyeing , *PARAFFIN wax , *TOLUIDINE blue , *COST effectiveness , *DIAGNOSIS ,RABBIT diseases - Abstract
Cystic-like structures similar to xenomas containing ovoid or piriform organisms were observed in brain and kidney of a one-month-old rabbit. Different dyes such as H&E, PAS, toluidine blue and trichrome blue were tested. Trichrome blue yielded the best results in terms of specificity, resolution and confirmation of microsporidia spores. This dye is easily adaptable to paraffin-embedded samples by not requiring additional equipment; it is rapid, cheap and could be used as a confirmatory technique for other microsporidiosis. [ABSTRACT FROM AUTHOR]
- Published
- 2011