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Environmental short amplicon sequencing, or metabarcoding, is commonly used to characterize the bacterial and fungal microbiota of cheese. Comparisons between different metabarcoding studies are complicated by the use of different gene markers. Here, we systematically compare different metabarcoding molecular targets using V3–V4 and V6–V8 regions of the bacterial 16S rDNA and fungal ITS1 and ITS2 regions. Taxonomic profiles varied depending on the molecular markers used. Based on data quality and detection capacity of the markers toward microorganisms usually associated with the dairy environment, the ribosomal regions V3–V4 and ITS2 were selected and further used to evaluate variability in the microbial ecosystem of terroir cheeses from the province of Quebec in Canada. Both fungal and bacterial ecosystem profiles were described for 32 different ready-to-eat bloomy-, washed- and natural-rind specialty cheese varieties. Among them, 15 were studied over two different production years. Using the Bray–Curtis dissimilarity index as an indicator of microbial shifts, we found that most variations could be explained by either a voluntary change in starter or ripening culture composition, or by changes in the cheesemaking technology. Overall, our results suggest the persistence of the microbiota between the two years studied—these data aid understanding of cheese microbiota composition and persistence during cheese ripening. [ABSTRACT FROM AUTHOR]

In many Western societies, support for policies concerning the secularization of the public sphere or the state often seems to be driven by secularized majority populations considered to be largely homogeneous. In this article, by contrast, I draw on the case of the Canadian province of Quebec to show that, as a fundamental element of conflicts over secularism, secularist activism emerges from particular generational dynamics, especially those of the so-called 'baby boomers'. My main argument is that while the baby boomers' collective experiences have shaped their secularist outlook, there are a variety of biographical trajectories and engagements with spirituality that the public image of this generation tends to hide. The article is based on biographical and ethnographic research carried out between 2012 and 2018. [ABSTRACT FROM AUTHOR]

Building from Blum and Dobrotić's conceptual framework, this paper shows that the decisions to reopen childcare centres and schools in the Canadian province of Quebec in 2020 were influenced by four goals: (1) protecting public health, (2) promoting academic success / fostering early education, (3) addressing social inequalities, and (4) helping parents to reconcile employment and care activities. Although the first three objectives were considered in the sequence of reopening, the government quickly chose to prioritize work-family reconciliation above other objectives. Quebec's tight sequence of reopening childcare centres and schools is not simply a consequence of evolving research showing lower risks associated with COVID-19 for young children, it also reflects the province's cultural norms toward mothers' employment and the high legitimacy of the state in subsidizing childcare. [ABSTRACT FROM AUTHOR]

Copyright of Canadian Foreign Policy Journal (CFPJ) is the property of Routledge and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

This article sheds light on the complexity of international climate change negotiations in a federal country, like Canada, where there is no clear attribution of full power over international negotiation concerning this issue. Climate change is a multi-level and multi-stakeholder issue, one that can only be tackled successfully if all actors, at all levels of government, are involved in the process. In recent years, Canadian provinces, especially Quebec, have become intensely involved in climate change paradiplomacy. That situation has led to a Canadian paradox where the Government of Quebec worked to respect the Kyoto Protocol and act accordingly, while Canada opted out of the Protocol in 2011. [ABSTRACT FROM AUTHOR]

Copyright of Canadian Tax Journal / Revue Fiscale Canadienne is the property of Canadian Tax Foundation and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)