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2. Quantification of biofilm exopolysaccharides using an in situ assay with periodic acid–Schiff reagent.

Author

Randrianjatovo-Gbalou, I., Girbal-Neuhauser, E., and Marcato-Romain, C.-E.

Subjects
*BIOFILMS, *MICROBIAL exopolysaccharides, *BIOLOGICAL assay, *SCHIFF bases, *BIOLOGICAL reagents, *BACILLUS licheniformis
Abstract

A novel approach to the quantification of extracellular polysaccharides in miniaturized biofilms presenting a wide variety of extracellular matrices was developed. The assay used the periodic acid–Schiff reagent and was first calibrated on dextran and alginate solutions. Then it was implemented on 24-h and 48-h biofilms from three strains known to produce different exopolymeric substances ( Pseudomonas aeruginosa , Bacillus licheniformis , Weissella confusa ). The assay allowed quantification of the total exopolysaccharides, taking into account possible interferences due to cells or other main expolymers of the matrix (eDNA, proteins). [ABSTRACT FROM AUTHOR]

Published
2016
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