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In present study, comparative andrological histopathologies of Cypermethrin (CP) (a chlorinated type II pyrethroid) and Lambda-Cyhalothrin (LN) (a fluorinated type II pyrethroid) insecticide exposure and their amelioration with post treatment with Extra Virgin Olive oil (EVO) in testes, epididymis and vas deferens were explored. Sixty male albino mice (12-14 weeks old) were randomly distributed in six groups (n=10): 1. Negative Control (NC) is vehicle control so received 0.1mL corn oil on day 1&2, no treatment (day 3) and 0.1mL corn oil (day 4-6) through gavage; 2. Positive Control (PC) (the olive oil EVO antidote group) were given 0.1mL corn oil on day 1&2, no treatment (day 3) and 0.1mL EVO (day 4-6); 3,4) CP and LN were treated with 0.1mL of their respective 5mg/kg insecticides dissolved in corn oil on day 1&2, no treatment (day 3) and 0.1mL corn oil (day 4-6); 5,6) CP+EVO and LN+EVO were given 0.1mL of their respective 5mg/kg insecticides on day 1&2, no treatment (day 3) and 0.1mL EVO (day 4- 6). Animals were dissected on day 7 to recover testes, epididymis and vas deferens. Excised organs were processed for serial sectioning and micrometry. Exposure with CP and LN results in severe damage to seminiferous tubules and interstitial tissues of testes and endothelium of epididymis and vas deferens. Toxic effects were depicted as empty spaces in seminiferous tubules, interstitial tissues and epididymis and vas deferens, wavy margins of seminiferous tubules and corpus epididymis and necrosis in spermatogonia. Simultaneously the mean cross-sectional area of seminiferous tubules was significantly (p<0.05) decreased in CP (10.34±3.00µm2) and LN (12.63±3.23µm²) than PC (15.30±6.25 µm²) and NC (14.69±4.96µm²) groups. The EVO post treatment groups CP+EVO and LN+EVO indicate signs of rehabilitation in the mean CSA of seminiferous tubules (13.04±5.45µm², 13.07±5.80µm²) and interstitial tissues (30.47±0.67µm², 33.60±1.23µm²) as well as the mean number of spermatogonia. There was significant (P≤0.05) decrease in the mean epithelial height of caput (22.78±0.56µm², 22.42±0.49µm²), corpus (20.29±0.42µm², 20.15±0.55µm²) and cauda epididymis convoluted tubing (18.29±0.59µm², 17.93±0.52µm²) and epithelial thickness of vas deferens (29.17±1.49µm², 28.91±0.88 µm²) in CP and LN groups. The recovery signs of extra virgin olive oil in CP+EVO and LN+EVO groups were observed as rehabilitation in epithelial height of caput (23.58±0.49µm², 23.04±0.51µm²), corpus (20.97±0.54µm², 20.69±0.55 µm²) and cauda epididymis (21.43±0.65 µm2, 20.13±0.70 µm²) and epithelial thickness of vas deferens (34.06±1.07µm², 31.24±0.96µm²). The results indicate that both insecticides inflicted almost similar histopathologies which were convincingly recovered by the olive oil post treatment. It is thus concluded that irrespective of the presence of fluoride the type II pyrethroids can inflict severe testicular, epididymal and vas deferens damage in the exposed animals whereas the olive oil has immense rehabilitative capacity and thus can be used against insecticide inflicted testicular, epididymal and vas deferens histopathologies. [ABSTRACT FROM AUTHOR]

Histopathological and micrometric alterations of mice liver and kidney against gastro-intestinal exposure of (lambda-cyhalothrin) (LCT) and (cypermethrin) (CYP) were investigated along with the rescuing potential of extra virgin olive oil (EVOO). The study comprised 60 male albino mice randomly distributed in 6 groups (n=10). The vehicle control group (VC) was maintained and treated with 0.1ml corn oil. The positive control group was treated with EVOO, whereas the (CYP) and (LCT) groups were treated with 5mg/kg of the respective insecticides for two consecutive days and corn oil for 4-6 days. The last 2 groups Cypermethrin+extra virgin olive oil (CYP+EVOO) and Lambda-cyhalothrin+extra virgin olive oil (LCT+EVOO) were treated with olive oil only on day 4-6 along with respective insecticide exposure on first two days. All animals were sacrificed on day 7 to recover body organs (Liver and Kidney). Results show various histopathological alterations both in Liver (enormously enlarged hepatocytes, disruption of hepatic cords, etc.) and kidney (lesions in the endothelial wall of bowman's capsule, endothelial cells apoptosis, etc.) on CYP and LCT exposures. Significant micrometric changes were also noted along with pathological signs. The olive oil post treatment groups have shown rehabilitative effects against the reported histopathologies. On the basis of these findings, it is concluded that both insecticides harbor hepato-renal histopathological potentials that are at least partially rescued on EVOO treatment. [ABSTRACT FROM AUTHOR]

The present study examines the thyro-parathroid toxicological impacts of a fluoridated (Lambda cyhalothrin) and a non-fluoridated (Cypermethrin) pyrethroid in male albino mice and the amelioration of these toxicities with post treatment with extra virgin olive oil (EVOO). Study plan included 6 groups. 1: Vehicle control (Cnt group) received 0.1 mL corn oil for days 1,2, 4-6) and 2: Positive control (Olv group) received 0.1 mL corn oil for days 1-3 and 0.1mL EVOO for days 4-6) 3: cypermethrin group (Cpr group) and 4: Lambda Cyhalothrin group (Lch group) received 5 mg/Kg cypermethrin or Lambda Cyhalothrin respectively for days 1-2, no treatment on day 3, 0.1mL corn oil for days 4-6) 5: Cypermethrin+Olive group (Cpr+Olv group) and 6: Lambda cyhalothrin+Olive group (Lch+Olv group) received 5mg/kg cypermethrin or Lambda Cyhalothrin respectively for days 1-2, no treatment on day 3, 0.1mL EVOO for days 4-6). Results of the present study indicate that both of the insecticides are toxic to the endocrine tissue examined in the study. However lambda cyhalothrin led to severe histopathological (Cords seemed to be merged and focal degenerations can also be observed in central and peripheral regions) and micrometric changes compared to the cypermethrin. Various significant alterations occurred in micrometric parameters, such as mean CSA of follicular cells, Para-follicular cells and follicles were significantly higher in lambda cyhalothrin groups as compared to cypermethrin group. The morphometric data also showed a significant (p≤0.05) decrease in mean cross-sectional area of follicular cells (Cnt: 51.33±1.05, Olv: 51.16±1.16, Cpr: 62.45±1.8, Lch: 61.00±2.1, Cpr+olv: 54.3±1.8 and Lch+olv: 55.2±1.7), CSA of follicles (Cnt: 2083±128.6, Olv: 2019.84±189.7, Cpr: 1946.6±94.03, Lch: 1712.6±139.6, Cpr+olv: 2041.7±114 and Lch+olv: 1483.3±134.4), Para-follicular cells (Cnt: 55±11.09, Olv: 51.76±9.0, Cpr: 73.8±15.7, Lch: 60.9±10.7, Cpr+olv: 54.1±8.8 and Lch+olv: 51.9±11.8) indicating that the fluoridation has contributed significantly to the pathological potential of this insecticide. On the other hand olive oil has been found to contain rescuing and rehabilitation potentials and ameliorated the mean CSA of follicular cells, CSA of follicles and parafollicualr cells, against the histopathologies of these insecticides. [ABSTRACT FROM AUTHOR]