1. Dual RNA-Seq uncovers the function of an ABC transporter gene in the host-pathogen interaction between Epinephelus coioides and Pseudomonas plecoglossicida.
- Author
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Tang, Ruiqiang, Zhao, Lingmin, Xu, Xiaojin, Huang, Lixing, Qin, Yingxue, Su, Yongquan, and Yan, Qingpi
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ATP-binding cassette transporters , *EPINEPHELUS , *PSEUDOMONAS , *IMMUNOSUPPRESSION , *GENES , *FISH mortality - Abstract
As an important pathogen in aquaculture, Pseudomonas plecoglossicida has caused heavy losses. The expression of an ABC transporter gene- L321_23611 of P. plecoglossicida at 18 °C was found significant higher than those at 28 °C by RNA-seq and qRT-PCR. RNAi significantly reduced the content of L321_ 23611 mRNA in P. plecoglossicida with a maximal decrease of 89.2%. Compared with the wild type strain, the infection of L321_23611- RNAi strain resulted in the reduction in mortality and the onset time delay of a kind of marine teleosts, Epinephelus coioides. The results of dual RNA-seq showed that the RNAi of L321_23611 resulted in a significant change in both pathogen and host transcriptome in the spleens of infected E. coioides. The result of GO and KEGG analysis from dual RNA-seq data showed both host genes of chemokine signaling pathway, coagulation and complement system, hematopoietic cell lineage pathway as well as hemoglobin complex GO term and pathogenic genes of bacterial-type flagellum-dependent cell mortality GO term and flagellar assembly, biosynthesis of amino acids and lysine biosynthesis systems pathways were mainly affected by L321_23611 gene of P. plecoglossicida. The results indicated that: 1. ABC transporter gene- L321_23611 was a virulent gene of P. plecoglossicida. 2. Both the activation of the host immune pathways and depression of pathogenic virulence-related pathways facilitated E. coioides to remove L321_23611 -RNAi strain than the wild type strain of P. plecoglossicida. • RNAi significantly reduced the content of L321_ 23611 mRNA of P. plecoglossicida. • RNAi of L321_23611 reduced the pathogenicity of P. plecoglossicida to E. coioides. • RNAi of L321_23611 significantly up-regulated host immune pathways. • RNAi of L321_23611 down-regulated some pathogenic genes of P. plecoglossicida. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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