Search

Your search keyword '"Chauveau, Antoine"' showing total 4 results
Start Over Author "Chauveau, Antoine" Topic protopine
4 results on '"Chauveau, Antoine"'

1. Placental Passage of Protopine in an Ex Vivo Human Perfusion System.

Author

Spiess, Deborah, Abegg, Vanessa Fabienne, Chauveau, Antoine, Treyer, Andrea, Reinehr, Michael, Oufir, Mouhssin, Duong, Elisa, Potterat, Olivier, Hamburger, Matthias, and Simões-Wüst, Ana Paula

Subjects
MATERNAL-fetal exchange, ALKALOIDS, PLACENTA, PLANT extracts, MOLECULAR structure, PERFUSION
Abstract

The placental passage of protopine was investigated with a human ex vivo placental perfusion model. The model was first validated with diazepam and citalopram, 2 compounds known to cross the placental barrier, and antipyrine as a positive control. All compounds were quantified by partially validated U(H)PLC-MS/MS bioanalytical methods. Protopine was transferred from the maternal to the fetal circuit, with a steady-state reached after 90 min. The study compound did not affect placental viability or functionality, as glucose consumption, lactate production, and beta-human chorionic gonadotropin, and leptin release remained constant. Histopathological evaluation of all placental specimens showed unremarkable, age-appropriate parenchymal maturation with no pathologic findings. [ABSTRACT FROM AUTHOR]

Published
2023
Full Text
View/download PDF

2. Placental Passage of Humulone and Protopine in an Ex Vivo Human Perfusion System#.

Author

Spiess, Deborah, Abegg, Vanessa Fabienne, Chauveau, Antoine, Treyer, Andrea, Reinehr, Michael, Oufir, Mouhssin, Duong, Elisa, Potterat, Olivier, Hamburger, Matthias, and Simões-Wüst, Ana Paula

Subjects
BIOLOGICAL models, CITALOPRAM, MATERNAL-fetal exchange, ALKALOIDS, CELL survival, GONADOTROPIN, PLACENTA, MASS spectrometry, DESCRIPTIVE statistics, PLANT extracts, MOLECULAR structure, PERFUSION, DIAZEPAM
Abstract

The placental passage of humulone and protopine was investigated with a human ex vivo placental perfusion model. The model was first validated with diazepam and citalopram, 2 compounds known to cross the placental barrier, and antipyrine as a positive control. All compounds were quantified by partially validated U(H)PLC-MS/MS bioanalytical methods. Only a small portion of humulone initially present in the maternal circuit reached the fetal circuit. The humulone concentration in the maternal circuit rapidly decreased, likely due to metabolization in the placenta. Protopine was transferred from the maternal to the fetal circuit, with a steady-state reached after 90 min. None of the study compounds affected placental viability or functionality, as glucose consumption, lactate production, beta-human chorionic gonadotropin, and leptin release remained constant. Histopathological evaluation of all placental specimens showed unremarkable, age-appropriate parenchymal maturation with no pathologic findings. [ABSTRACT FROM AUTHOR]

Published
2021
Full Text
View/download PDF

3. Alkaloids in commercial preparations of California poppy – Quantification, intestinal permeability and microbiota interactions.

Author

Chauveau, Antoine, Geirnaert, Annelies, Babst, Angela, Treyer, Andrea, Lacroix, Christophe, Hamburger, Matthias, and Potterat, Olivier

Subjects
*GUT microbiome, *ALKALOIDS, *SHORT-chain fatty acids, *PAPAVERACEAE, *BACTERIAL inactivation
Abstract

California poppy products are commonly used for the treatment of nervousness, anxiety and sleeping disorders. Pharmacologically relevant constituents include the main alkaloids californidine, escholtzine and protopine. However, only limited information is available about the alkaloid content in commercial preparations and their intestinal absorption. Moreover, a possible metabolization of these alkaloids by the gut microbiota, and their impact on microbial activity and viability have not been investigated. Californidine, escholtzine and protopine were quantified by UHPLC-MS/MS in eight commercial California poppy products. The intestinal permeability of alkaloids was studied in Caco-2 cell as a model for absorption in the small intestine. The gut microbial biotransformation was explored in artificial gut microbiota from the in vitro PolyFermS model. In addition, the impact of these alkaloids and a California poppy extract on the microbial production of short-chain fatty acids (SCFAs) and the viability of microbiota was investigated. Contents of californidine, escholtzine and protopine in California poppy products were in the ranges of 0.13–2.55, 0.05–0.63 and 0.008–0.200 mg/g, respectively. In the Caco-2 cell model, californidine was low-to-moderately permeable while escholtzine and protopine were highly permeable. An active transport process was potentially involved in the transfer of the three alkaloids. The three compounds were not metabolized by the artificial gut microbiota over 24 h. Neither the California poppy extract nor the alkaloids markedly impacted microbial SCFA production and bacterial viability. • Alkaloidal content was determined in 8 commercial products of California poppy. • Intestinal permeability and gut microbiota interactions of alkaloids were assessed. • Contents in alkaloids differed strongly between the products of California poppy. • Californidine was lowly permeable, other alkaloids were highly permeable. • Compounds were not metabolized by the microbiota and did not impact their viability. [ABSTRACT FROM AUTHOR]

Published
2023
Full Text
View/download PDF

4. Placental Passage of Humulone and Protopine in an Ex Vivo Human Perfusion System#.

Author

Spiess, Deborah, Abegg, Vanessa Fabienne, Chauveau, Antoine, Treyer, Andrea, Reinehr, Michael, Oufir, Mouhssin, Duong, Elisa, Potterat, Olivier, Hamburger, Matthias, and Simões-Wüst, Ana Paula

Subjects
*BIOLOGICAL models, *CITALOPRAM, *MATERNAL-fetal exchange, *ALKALOIDS, *CELL survival, *GONADOTROPIN, *PLACENTA, *MASS spectrometry, *DESCRIPTIVE statistics, *PLANT extracts, *MOLECULAR structure, *PERFUSION, *DIAZEPAM
Abstract

The placental passage of humulone and protopine was investigated with a human ex vivo placental perfusion model. The model was first validated with diazepam and citalopram, 2 compounds known to cross the placental barrier, and antipyrine as a positive control. All compounds were quantified by partially validated U(H)PLC-MS/MS bioanalytical methods. Only a small portion of humulone initially present in the maternal circuit reached the fetal circuit. The humulone concentration in the maternal circuit rapidly decreased, likely due to metabolization in the placenta. Protopine was transferred from the maternal to the fetal circuit, with a steady-state reached after 90 min. None of the study compounds affected placental viability or functionality, as glucose consumption, lactate production, beta-human chorionic gonadotropin, and leptin release remained constant. Histopathological evaluation of all placental specimens showed unremarkable, age-appropriate parenchymal maturation with no pathologic findings. [ABSTRACT FROM AUTHOR]

Published
2021
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results