1. E74 like ETS transcription factor 3 (ELF3) is a negative regulator of epithelial- mesenchymal transition in bladder carcinoma.
- Author
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Gondkar K, Patel K, Krishnappa S, Patil A, Nair B, Sundaram GM, Zea TT, and Kumar P
- Subjects
- Biomarkers, Tumor, Cell Line, Tumor, Cell Movement, Cell Proliferation, Computational Biology methods, DNA-Binding Proteins metabolism, Gene Expression Profiling, Humans, Immunohistochemistry, Multigene Family, Neoplasm Grading, Neoplasm Staging, Prognosis, Proto-Oncogene Proteins c-ets metabolism, Transcription Factors metabolism, Transcriptome, Urinary Bladder Neoplasms metabolism, Urinary Bladder Neoplasms mortality, DNA-Binding Proteins genetics, Epithelial-Mesenchymal Transition genetics, Gene Expression Regulation, Neoplastic, Proto-Oncogene Proteins c-ets genetics, Transcription Factors genetics, Urinary Bladder Neoplasms etiology, Urinary Bladder Neoplasms pathology
- Abstract
Background: Transcription factors are commonly deregulated in various cancers. Here, we evaluated role of ELF3 in pathogenesis of bladder carcinoma (BCa)., Materials and Methods: We confirmed ELF3 expression pattern in BCa cell lines using western blot; and in different grades of tumors using Immunohistochemistry. Cell invasion assay was employed to demonstrate potential role of ELF3 in EMT., Results and Conclusion: ELF3 showed selective expression in low-grade cell lines and tumor tissues. Overexpression of ELF3 in mesenchymal cell line UMUC3 resulted in reduced invasion and decreased expression of mesenchymal markers. We observed association of low ELF3 expression with increased risk and overall poor survival using publicly available data. ELF3-modulated reversal of EMT might be a useful strategy in the treatment of bladder cancer.
- Published
- 2019
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