1. Chemoselective Proteomics, Zinc Fingers, and a Zinc(II) Model for H 2 S Mediated Persulfidation.
- Author
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Stoltzfus AT, Ballot JG, Vignane T, Li H, Worth MM, Muller L, Siegler MA, Kane MA, Filipovic MR, Goldberg DP, and Michel SLJ
- Subjects
- Humans, Protein Processing, Post-Translational, Hydrogen Sulfide chemistry, Hydrogen Sulfide metabolism, Zinc chemistry, Zinc Fingers, Proteomics, Sulfides chemistry
- Abstract
The gasotransmitter hydrogen sulfide (H
2 S) is thought to be involved in the post-translational modification of cysteine residues to produce reactive persulfides. A persulfide-specific chemoselective proteomics approach with mammalian cells has identified a broad range of zinc finger (ZF) proteins as targets of persulfidation. Parallel studies with isolated ZFs show that persulfidation is mediated by ZnII , O2 , and H2 S, with intermediates involving oxygen- and sulfur-based radicals detected by mass spectrometry and optical spectroscopies. A small molecule ZnII complex exhibits analogous reactivity with H2 S and O2 , giving a persulfidated product. These data show that ZnII is not just a biological structural element, but also plays a critical role in mediating H2 S-dependent persulfidation. ZF persulfidation appears to be a general post-translational modification and a possible conduit for H2 S signaling. This work has implications for our understanding of H2 S-mediated signaling and the regulation of ZFs in cellular physiology and development., (© 2024 Wiley-VCH GmbH.)- Published
- 2024
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