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Monolayer cultures of hepatocytes were prepared. Supplementation of the incubation medium with bovine serum albumin increasing from 150 mg% to 6% resulted in an increase in retained and total newly synthesised proteins. No clear effects on secretion of soluble proteins were observed. Hydrocortisone supplementation of the medium at 13.75 and 27.50 nmol/l resulted in both enhanced synthesis and secretion of soluble proteins, an effect found not to be a function of concentration. Serum inhibited the effect. Hydrocortisone supplementation resulted in enhanced [3H]leucine uptake by the cells, inhibited in the presence of anti-rat albumin serum. Hydrocortisone supplementation had no effect on the inhibition of synthesis and secretion that resulted from supplementation of the medium with anti-rat albumin serum.

1. Interstitial fluid (obtained from capsules implanted subcutaneously) and plasma were obtained from rats before and after heat and cold exposure and exercise. 2. In the case of plasma, cold and heat exposure and exercise decreased A/G ratios. Albumin concentrations also decreased after heat and cold and protein concentration increased after exercise. 3. Cold decreased interstitial protein and albumin concentrations and A/G ratios. Heat exposure increased protein concentration and decreased the A/G ratio. Exercise decreased decreased protein concentrations. 4. The results are discussed in relation to previous findings.