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2 results on '"Combe CW"'

1. Toward Increased Reliability, Transparency, and Accessibility in Cross-linking Mass Spectrometry.

Author

Leitner A, Bonvin AMJJ, Borchers CH, Chalkley RJ, Chamot-Rooke J, Combe CW, Cox J, Dong MQ, Fischer L, Götze M, Gozzo FC, Heck AJR, Hoopmann MR, Huang L, Ishihama Y, Jones AR, Kalisman N, Kohlbacher O, Mechtler K, Moritz RL, Netz E, Novak P, Petrotchenko E, Sali A, Scheltema RA, Schmidt C, Schriemer D, Sinz A, Sobott F, Stengel F, Thalassinos K, Urlaub H, Viner R, Vizcaíno JA, Wilkins MR, and Rappsilber J

Subjects
Guidelines as Topic, Humans, International Cooperation, Mass Spectrometry instrumentation, Mass Spectrometry standards, Protein Conformation, Protein Interaction Mapping methods, Proteomics instrumentation, Proteomics standards, Reproducibility of Results, Cross-Linking Reagents chemistry, Mass Spectrometry methods, Proteins ultrastructure, Proteomics methods
Abstract

Cross-linking mass spectrometry (MS) has substantially matured as a method over the past 2 decades through parallel development in multiple labs, demonstrating its applicability to protein structure determination, conformation analysis, and mapping protein interactions in complex mixtures. Cross-linking MS has become a much-appreciated and routinely applied tool, especially in structural biology. Therefore, it is timely that the community commits to the development of methodological and reporting standards. This white paper builds on an open process comprising a number of events at community conferences since 2015 and identifies aspects of Cross-linking MS for which guidelines should be developed as part of a Cross-linking MS standards initiative., (Copyright © 2020.)

Published
2020
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2. An integrated workflow for crosslinking mass spectrometry.

Author

Mendes ML, Fischer L, Chen ZA, Barbon M, O'Reilly FJ, Giese SH, Bohlke-Schneider M, Belsom A, Dau T, Combe CW, Graham M, Eisele MR, Baumeister W, Speck C, and Rappsilber J

Subjects
Cytosol chemistry, Humans, K562 Cells, Models, Molecular, Peptide Fragments chemistry, Protein Conformation, Software, Mass Spectrometry methods, Protein Interaction Mapping methods, Proteins chemistry, Proteomics methods
Abstract

We present a concise workflow to enhance the mass spectrometric detection of crosslinked peptides by introducing sequential digestion and the crosslink identification software xiSEARCH. Sequential digestion enhances peptide detection by selective shortening of long tryptic peptides. We demonstrate our simple 12-fraction protocol for crosslinked multi-protein complexes and cell lysates, quantitative analysis, and high-density crosslinking, without requiring specific crosslinker features. This overall approach reveals dynamic protein-protein interaction sites, which are accessible, have fundamental functional relevance and are therefore ideally suited for the development of small molecule inhibitors., (© 2019 The Authors. Published under the terms of the CC BY 4.0 license.)

Published
2019
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