1. Immunoaffinity Mass Spectrometry Diagnostic Tests for Multi-Biomarker Assays.
- Author
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Bringans S, Casey T, Ito J, Lumbantobing T, O'Neill R, and Lipscombe R
- Subjects
- Mass Spectrometry methods, Biomarkers analysis, Diagnostic Tests, Routine, Proteins, Antibodies
- Abstract
Immunoaffinity mass spectrometry as an approach for diagnostic biomarker assays combines the advantages of antibody selectivity with the multiplexing and analytical performance of mass spectrometry. A method has been developed to detect and quantify three protein biomarkers for a diabetic kidney disease prognostic assay, PromarkerD. The methodology reflects an immunoaffinity approach compatible with higher throughput and robust clinical application. After preparation and purification of antibody-bead conjugates for the three target proteins, an immunoaffinity capture step provides a solution for reduction, alkylation, and digestion on-bead. Targeted mass spectrometry provides a quantitative measure of each biomarker in a rapid 8 min run using a microflow LCMS workflow., (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
- Published
- 2023
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