1. Permuteins of interleukin 1 beta--a simplified approach for the construction of permutated proteins having new termini.
- Author
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Horlick RA, George HJ, Cooke GM, Tritch RJ, Newton RC, Dwivedi A, Lischwe M, Salemme FR, Weber PC, and Horuk R
- Subjects
- Amino Acid Sequence, Base Sequence, Cell Line, Circular Dichroism, Escherichia coli genetics, Humans, Interleukin-1 chemistry, Molecular Sequence Data, Mutagenesis, Site-Directed, Polymerase Chain Reaction, Protein Conformation, Radioligand Assay, Recombinant Proteins chemical synthesis, Interleukin-1 genetics, Protein Engineering methods
- Abstract
A technique for the rapid and simple generation of permutated versions of the interleukin-1 beta (IL-1 beta) gene is described. In this method, the human IL-1 beta cDNA is twice amplified by the polymerase chain reaction (PCR) and the resulting DNA fragments are ligated in tandem. Between the two genes, the DNA sequence encodes a short four amino acid loop to link the native N- and C-terminal ends of the IL-1 beta protein. By using PCR amplification from this starting template, a new version of the IL-1 beta cDNA was obtained that encodes a permutated form of the IL-1 beta protein where the new N- and C-terminal amino acids correspond to residues 65 and 64 of the native IL-1 beta sequence, respectively. The name 'permutein' is proposed to describe proteins generated by this technology. The molecular profile (IL-1 receptor binding, biologic activity and solution properties) of the IL-1 permutein produced by this technology, permutein 65/64, is shown to be identical to that of native IL-1 beta. The approach should be useful to define further the structural features of this protein that are important for its function.
- Published
- 1992
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