1. Chemical characterization and antioxidant potential of ecuadorian propolis.
- Author
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Cuesta-Rubio O, Hernández IM, Fernández MC, Rodríguez-Delgado I, De Oca Porto RM, Piccinelli AL, Celano R, and Rastrelli L
- Subjects
- Antioxidants chemistry, Antioxidants pharmacology, Chromatography, High Pressure Liquid, Ecuador, Flavonoids chemistry, Methanol, Diterpenes chemistry, Propolis chemistry, Triterpenes analysis
- Abstract
The chemical composition and the antioxidant potential of Ecuadorian propolis samples (n = 19) collected in different provinces were investigated. HPLC-DAD-ESI/MS
n and GC-EI-MS analysis of the methanol extracts enabled us to define six types of Ecuadorian propolis based on their secondary metabolite composition. 68 compounds were identified, 59 of which are reported for the first time in Ecuadorian propolis. The detected compounds include flavonoids, diterpenes, triterpenes, organic acid derivatives, alkylresorcinol derivatives and nemorosone. Plants belonging to genera Populus, Mangifera and Clusia seemed to be vegetable sources employed by bees to produce Ecuadorian propolis. Total phenolic content and antioxidant activity of propolis extracts were determined by the Folin-Ciocalteu assay and 2,2-diphenyl-1-picrylhydrazyl and ferric reducing/antioxidant potential assays, respectively. As expected, the variable chemical composition affected the differences in terms of antioxidant potential., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Ltd. All rights reserved.)- Published
- 2022
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