1. The Responses of Arabidopsis Early Light-Induced Protein2 to Ultraviolet B, High Light, and Cold Stress Are Regulated by a Transcriptional Regulatory Unit Composed of Two Elements.
- Author
-
Natsuki Hayami, Yusaku Sakai, Mitsuhiro Kimura, Tatsunori Saito, Mutsutomo Tokizawa, Satoshi Iuchi, Yukio Kurihara, Minami Matsui, Mika Nomoto, Yasuomi Tada, and Yamamoto, Yoshiharu Y.
- Subjects
- *
ARABIDOPSIS thaliana , *PLANT proteins , *PROMOTERS (Genetics) , *GENETIC transcription in plants , *EFFECT of ultraviolet radiation on plants , *EFFECT of light on plants , *EFFECT of cold on plants , *LEUCINE zippers , *PHYSIOLOGY - Abstract
The Arabidopsis (Arabidopsis thaliana) Early Light-Induced Protein (ELIP) is thought to act as a photoprotectant, reducing the damaging effects of high light (HL). Expression of ELIP2 is activated by multiple environmental stresses related to photoinhibition. We have identified putative regulatory elements in an ELIP2 promoter using an octamer-based frequency comparison method, analyzed the role of these elements using synthetic promoters, and revealed a key transcriptional regulatory unit for ultraviolet B (UV-B) radiation, HL, and cold stress responses. The unit is composed of two elements, designated as Elements A (TACACACC) and B (GGCCACGCCA), and shows functionality only when paired. Our genome-wide correlation analysis between possession of these elements in the promoter region and expression profiles in response to UV-B, HL, and cold suggests that Element B receives and integrates these multiple stress signals. In vitro protein-DNA binding assays revealed that LONG HYPOCOTYL5 (HY5), a basic domain-Leucine zipper transcription factor, directly binds to Element B. In addition, mutant analysis of HY5 showed partial involvement in the UV-B and HL responses but not in the cold stress response. These results suggest that signals for UV-B, HL, and cold stress join at Element B, which recognizes the signals of multiple transcription factors, including HY5. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF