1. Morphological and Biochemical Characteristics of Prostate Hyperplasia during Sulpiride Treatment.
- Author
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Tsvetkov IS, Kosyreva AM, Mkhitarov VA, Postovalova EA, Khochanskiy DN, Makarova OV, Bredova OY, and Ostrov VF
- Subjects
- Acinar Cells drug effects, Acinar Cells metabolism, Acinar Cells pathology, Animals, Disease Models, Animal, Epithelial Cells drug effects, Epithelial Cells metabolism, Epithelial Cells pathology, Gene Expression, Humans, Injections, Intramuscular, Ki-67 Antigen genetics, Ki-67 Antigen metabolism, Male, Prolactin blood, Prostate metabolism, Prostate pathology, Prostate-Specific Antigen blood, Prostatic Hyperplasia blood, Prostatic Hyperplasia chemically induced, Prostatic Hyperplasia genetics, Rats, Rats, Sprague-Dawley, Testosterone blood, Dopamine Antagonists administration & dosage, Prolactin genetics, Prostate drug effects, Prostate-Specific Antigen genetics, Prostatic Hyperplasia pathology, Sulpiride administration & dosage
- Abstract
We studied morphological changes in the prostate ventral lobe, proliferative activity of the epithelium in prostate acini, and the levels of prolactin and prostate-specific antigen in the blood serum of Sprague-Dawley rats after repeated injections of sulpiride in a dose of 40 mg/ kg over 30 and 60 days and in 10 and 30 days after withdrawal. Morphological and morphometrical analysis of hyperplastic changes in the prostate ventral lobe was performed. Ki-67
+ proliferating epithelial cells in the acini were counted. The dynamics of serum concentrations of prolactin and prostate-specific antigen was evaluated by ELISA. Morphological and morphometrical analysis and evaluation of the content of Ki-67+ cells demonstrated epithelium hyperplasia in the prostate ventral lobe after sulpiride treatment for 30 or 60 days and in 10 days after withdrawal, but serum level of prostate-specific antigen did not differ from the control. After 60-day sulpiride treatment and in 30 days after withdrawal, pronounced hyperplastic changes of prostate and elevated concentrations of prostate-specific antigen (but not prolactin) were observed. Thus, administration of sulpiride (40 mg/kg) to Sprague-Dawley rats for 60 days allows, by morphological criteria and serum level of prostate-specific antigen, to model stable hyperplastic changes in the prostate corresponding to benign prostatic hyperplasia in humans.- Published
- 2020
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