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Background: Triple-negative breast cancer (TNBC) is the most malignant type of breast cancer. MicroRNAs (miRs) and their corresponding molecular targets are associated with the occurrence and development of various human malignancies. However, the roles of the microRNA-153 (miR-153) and zinc finger E-box-binding homeobox 2 (ZEB2)-induced epithelial–mesenchymal transition (EMT) in TNBC and predictive effect of miR-153 on the prognosis of TNBC have not been fully elucidated. Materials and methods: Relative miR-153 expression level was examined by RT-qPCR assay in TNBC tissues of 60 patients and TNBC cell lines (SKBR3, BT-549 and MDA-MB-231). Cell proliferation ability, invasion ability and migration ability were measured by CCK8 assay, Transwell invasion assay and wound healing assay, respectively. Luciferase reporting experiment was used to confirm that there was a miR-153-binding site in ZEB2 3ʹ-UTR. The expression of ZEB2 in tissues and its relationship with miR-153 were analyzed with immunohistochemistry method. Relative ZEB2, E-cadherin, N-cadherin and Vimentin mRNA and protein expression levels were observed with RT-qPCR and Western blot, respectively. Based on risk factors, a prognostic model was established according to the Cox proportional risk model, and the prognostic risk factors of TNBC patients were predicted and analyzed. Results: The expression of miR-153 in TNBC tissues and cells was declined (all P<0.01), and upregulation of miR-153 inhibited proliferation, invasion and migration of TNBC cells (all P<0.01). In addition, miR-153 regulated ZEB2/EMT link in TNBC, and ZEB2 overexpression reversed the tumor-suppressive effect of miR-153 in TNBC. Moreover, miR-153 was an independent predictive factor that was associated with excellent prognosis in TNBC patients. Conclusion: miR-153 may inhibit TNBC proliferation, invasion and migration by regulating ZEB2/EMT link. Therefore, miR-153 is expected to be a molecular target and prognostic marker for TNBC. [ABSTRACT FROM AUTHOR]

Objective: To study the effects of preoperative enteral nutrition (EN) on postoperative recent nutritional status (PRNS) in patients with Siewert II and III adenocarcinomas of esophagogastric junction (AEG) after neoadjuvant chemoradiotherapy (NCRT). Methods: A total of 66 patients with resectable AEG (Siewert II and III) were randomly divided into two groups. The trial group accepted oral nutrition supplementation (ONS) for 7 days before surgery while the control not. Results: Nutrition indexes were higher in trial group after surgery whereas the opposite was true for the diamine oxidase (DAO) and D-lactate (P < 0.05). The rate of malnutrition and nutritional risk became lower in trial group on the 8th day after surgery (P < 0.05). Injury levels of intestinal mucosa were more severe among control group. The recent prognosis was better in trial group. For patients with or without nutritional risks at admission, the PRNS and recent prognosis were improved by preoperative EN. Logistic regression analysis suggested that preoperative EN could be an independent protective factor of PRNS. Conclusions: Preoperative EN may improve the PRNS and recent prognosis of patients with Siewert II and III AEG after NCRT. [ABSTRACT FROM AUTHOR]

Vav3 is associated with tumor growth, apoptosis, invasion, metastasis and angiogenesis. In this study, we detected the expression of Vav3 in gastric cancer tissues, and explored its role in invasion, metastasis and prognosis of gastric cancer. Vav3, MMP-2, MMP-9, TIMP-1 and TIMP-2 in primary lesion and pericarcinous tissues were tested with Immunohistochemistry and Western blot. Results showed a higher expression of Vav3 in primary lesion than in pericarcinous tissue, and the expression of Vav3 was significantly correlated with MMP-2, MMP-9 and TIMP-1 in gastric cancer tissues. Overexpression of Vav3 was associated with poorer differentiation, advanced clinical stage, more significant infiltration depth, lymphatic metastasis, and perineural invasion. Results of Kaplan-Meier verified that overexpression of Vav3 was related to poorer prognosis and shorter survival time. Moreover, Cox proportional hazard model revealed that overexpression of Vav3 was an independent risk factor of prognosis for patients with gastric cancer. In all, we conclude that overexpression of Vav3 is an independent risk factor for prognosis of gastric cancer, and can be used as a prognostic indicator. This may be because that Vav3 could regulate genes which associated with the invasion and metastasis. [ABSTRACT FROM AUTHOR]

Gastrointestinal (GI) cancers, one of the most lethal cancers nowadays, have caused millions of deaths. The tumor microenvironment (TME) is a complex ecosystem containing multiple cells, such as immune cells, cancer cells, and endothelial cells. Tumor-associated macrophages (TAMs), the predominant cells infiltrating the TME, have been verified to play a crucial role in regulating tumor progression. Noncoding RNAs (ncRNAs) are involved in mediating tumor-infiltrating immune cells, including TAMs. GI cancer cells produce various ncRNAs to modulate TAM infiltration and polarization. Meanwhile, ncRNAs play a pivotal role in macrophage and cancer cell metabolic reprogramming. Furthermore, macrophages mediate the impact of ncRNAs on chemoresistance, metastasis, and progression of GI cancer. In this review, we summarize recent research advances in the crosstalk between macrophages and GI cancer regulated by ncRNAs. Moreover, we propose the promising role of ncRNAs as potential targets and prognostic biomarkers in cancer treatment, hoping to facilitate the identification of new targets for immunotherapy. [Display omitted] • NcRNAs mediate TAMs infiltration, polarization, and metabolic reprogramming in gastrointestinal cancer. • TAMs regulate the chemoresistance, metastasis, and progression of gastrointestinal cancer through exosomal ncRNAs. • The interaction between ncRNAs and TAMs provide novel treatment strategy for clinical application. [ABSTRACT FROM AUTHOR]

The purpose of this study was to analyze the influencing factors of BVI in advanced gastric cancer and explore the factors affecting the prognosis of advanced gastric cancer, so as to accurately evaluate the disease status and enable patients to receive effective treatment. We retrospectively analyzed 622 cases with complete data and successful follow-up. BVI was found in 144 of the 622 patients with advanced gastric cancer, with a detection rate of 23.15%. BVI was closely related to the differentiation degree, infiltration depth and lymph node metastasis of advanced gastric cancer, (P < 0.05). Gender, age, tumor location, tumor size, Lauren classification, tumor M stage, and clinical TNM stage were not the influencing factors of BVI in patients with advanced gastric cancer (P > 0.05). The 5-year survival rate of patients in the positive group of BVI was 34.72%. The 5-year survival rate of patients with advanced gastric cancer was correlated with BVI, Lauren classification, depth of invasion, lymph node metastasis, and clinical TNM staging, (P < 0.05). The 5-year survival rate was independent of gender, age, tumor location, tumor size, tumor tissue differentiation, and M stage (P > 0.05). The results of multi-factor analysis showed that BVI, N stage and clinical TNM stage were independent predictors of prognosis in patients with advanced radical gastric cancer. By analyzing the stage and related prognostic factors of resectable advanced gastric cancer, we found that BVI was not only closely related to lymph node metastasis, but also an independent predictor of prognosis of advanced gastric cancer. As this study was only a single-center retrospective study, there may be a selective bias in clinical data. So large-scale and multi-center collaboration is needed to further explore the influencing factors of BVI in the progression of gastric cancer. [ABSTRACT FROM AUTHOR]

Researches have shown that miR-425-5p expression altered in lung, esophageal, and glial cancer. Similarly, in our previous study, we found miR-425-5p expression was up-regulated in GC cells and could promote gastric cancer (GC) cell invasion and metastasis. However, the functional mechanism and the prognostic significance of miR-425-5p in GC remain unclear. Therefore, the present study examined miR-425-5p expression in GC tissues and also evaluated miR-425-5p of the therapeutic and prognostic value. Moreover, with interference of miR-425-5p expression in cell lines, we investigated the molecular mechanism of miR-425-5p and the expression level was higher in GC tissues comparing with that in gastric tumor-adjacent mucosa. These results suggested that miR-425-5p over-expression may be associated with depth of invasion and TNM stages and can be a prognostic marker of poor outcome. After inhibition of miR-425-5p expression in MKN45 cells, the cell activity was weakened. The number of G0/G1 cells increased while G2/M and S cells decreased, and the cell apoptotic rates elevated ( P < 0.05). Some proliferation and apoptosis related genes were altered ( P < 0.05). Consequently, miR-425-5p can be considered as a marker of poor prognosis and it is probably involved in GC cell proliferation and apoptosis by regulating some of the genes which participate these processes. [ABSTRACT FROM AUTHOR]