1. SCALE-UP OF A FLUIDIZED BED REACTOR OPERATED WITH POROUS GLASS CARRIERS
- Author
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Ch. Schmatz, Hermann Katinger, N. Zach, O. Doblhoff, F. Unterluggauer, Manfred Reiter, G. Kral, and G. Blüml
- Subjects
Pilot plant ,Materials science ,Volume (thermodynamics) ,business.industry ,Fluidized bed ,Nuclear engineering ,SCALE-UP ,Process control ,Porous glass ,Process engineering ,business ,Dilution ,Batch phase - Abstract
The IAM modular fluidized bed reactor was scaled up from laboratory size (7 1) to pilot scale (80 1). Hardeware was designed to fit the modular IAM pilot plant structure and software was adapted to the new reactor design. The new reactor was tested using porous glass beads and CHO cells as model cell line. After inoculation and batch phase a continuous perfusion system using protein free-medium was set up and operated over a period of three weeks. At the final dilution rate (approx. 12 times the carrier volume) a cell density up to 5 × 10 7 cells/ml carrier bed could be achieved. Fermentation control was achieved using an industrial direct digital process control system (Honeywell TDC 3000).
- Published
- 1992
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