1. Longan flower proanthocyanidins induce apoptosis in HT-29 colorectal carcinoma spheroids.
- Author
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Chung YC, Chiang HC, Chang H, Lin CC, Lo LT, Wang AY, Chou KF, and Hsu CP
- Subjects
- Antineoplastic Agents, Phytogenic pharmacology, Cell Proliferation drug effects, Colorectal Neoplasms drug therapy, Colorectal Neoplasms metabolism, ErbB Receptors metabolism, Humans, Phosphorylation, Proto-Oncogene Proteins c-akt metabolism, Spheroids, Cellular drug effects, Spheroids, Cellular metabolism, Tumor Cells, Cultured, Apoptosis drug effects, Colorectal Neoplasms pathology, Flowers chemistry, Plant Extracts pharmacology, Proanthocyanidins pharmacology, Sapindaceae chemistry, Spheroids, Cellular pathology
- Abstract
Aim of Study: Proanthocyanidin-rich longan flower extract (LFP) has been previously shown to inhibit the proliferation and anchorage-independent growth in soft agar of two colorectal carcinoma (CRC) cells in vitro. In this report, we further examined the effects of LFP in a CRC spheroid model., Materials and Methods: A liquid-overlay assay employing HT-29 spheroids was used to evaluate the effects of LFP on cancer cell tumorigenesis, viability, and apoptosis. Associated effects on signaling path ways (epidermal growth factor receptor [EGFR], Akt) and apoptotic regulators were measured using Western blot., Results: Treatment with LFP up to 200 μg/ml inhibited tumor growth in a dose-dependent manner and induced prominent apoptosis as measured by annexin V staining. Cells treated with LFP showed decreased EGFR and Akt phosphorylation with decreased expression of B-cell lymphoma 2., Conclusion: The ability of LFP to induce apoptosis in CRC spheroids warrants further investigation of its composition and identification of tumor-active components., Competing Interests: There are no conflicts of interest
- Published
- 2018
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