1. ProAKAP4 as Indicator of Long-Lasting Motility Marker in Post-Thaw Conditions in Stallions.
- Author
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Dordas-Perpinyà, Marta, Yánez-Ortiz, Iván, Sergeant, Nicolas, Mevel, Vincent, Catalán, Jaime, Bruyas, Jean-François, Miró, Jordi, and Briand-Amirat, Lamia
- Subjects
SEMEN ,STALLIONS ,MEMBRANE potential ,ENZYME-linked immunosorbent assay ,MITOCHONDRIAL membranes ,CYTOSKELETAL proteins - Abstract
Simple Summary: ProAKAP4, as a AKAP4 precursor, is the main protein of the spermatozoa flagellum in mammals. AKAP4, A-kinase anchor protein, is involved in sperm motility. The objective of the present study was to evaluate the evolution of proAKAP4 in horse semen post-thawing conditions during 3 h and its relationship with motility descriptors and mitochondrial membrane potential in the same conditions. The evolution in post-thawing conditions is the same either in proAKAP4 and motility parameters which gather that proAKAP4 can predict the evolution of the motility over the time. ProAKAP4, a precursor of AKAP4 (A-kinase anchor protein) found in the flagellum of mammalian and non-mammalian spermatozoa, serves as a structural protein with established correlations to motility parameters across diverse species. This study aimed to determine the proAKAP4 level evolution in thawed stallion semen over a 3 h period, examining its correlation with motility descriptors and mitochondrial membrane potential. Utilizing sixteen ejaculates from four French warmblood stallions, this study involved maintaining thawed samples at 37 °C for 3 h, conducting proAKAP4 enzyme-linked immunosorbent assays (ELISA), computer-assisted sperm analysis (CASA), and mitochondrial membrane potential by JC-1 probe and flow cytometry at 0, 1, and 3 h post-thawing. The findings indicate significant positive correlations (p ≤ 0.05) between proAKAP4 levels and sperm total or progressive motility at all time points analyzed. Spermatozoa velocity descriptors (VAP, VCL, VSL) and spermatozoa lateral head displacement (ALH) display positive correlations (p ≤ 0.05) with ProAKAP4 at the 0 h post-thawing. ProAKAP4 concentration exhibits no discernible difference between batches with or without a cryoprotectant. Notably, proAKAP4 consumption remains insignificant within the initial hour after thawing but becomes significant (p ≤ 0.05) between 1 and 3 h post-thawing. In summary, proAKAP4 demonstrates positive correlations with total and progressive motility in stallion semen for up to 3 h after thawing, albeit showing a noticeable decrease starting from the first hour post-thawing, indicating a progressive consumption as a result of spermatozoa motile activity. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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