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Keywords: AD; biogas; inoculum to substrate ratio; lignocellulosic; pretreatment; rice straw EN AD biogas inoculum to substrate ratio lignocellulosic pretreatment rice straw 549 559 11 06/01/22 20220501 NES 220501 1 Introduction 1.1 Rice straw biomass Rice ( I Oryza sativa i ) is the staple food for more than three billion people across the world ([10]). 3.2 Effect of pretreatment for ISR 0.5 Alkaline, electro-hydrolysis, and alkaline electro-hydrolysis pretreatment of rice straw gave appreciable higher biogas yield in comparison to control (untreated). From the literature, we found alkaline pretreatment ([16]; [19]; [20]; [24]) and electro-hydrolysis pretreatment ([12]; [20]) of rice straw, but combined pretreatment of alkaline electro-hydrolysis has not been reported. 3 Result and discussion The main aim of this study was to investigate the biogas yield of alkaline electro-hydrolysis pretreated rice straw and find the effects of different inoculum to substrate ratios (ISR) on biogas generation using untreated and pretreated rice straw. [Extracted from the article]