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Introduction: Immaturity of the ovarian follicles results in an infertility citation called POF or Premature Ovarian Failure. Women with this disorder lack graffian follicles so they don't have any oocytes for ovulation and therefore go through menopause before the age of 40 and have high levels of gonadotropin hormones (FSH & LH). On the other hand, Follicle stimulating hormone has a critical role in the maturation of the ovarian follicles from the antral to the graffian stage. Materials and Methods: FSH will start a signaling cascade in the granulosa cells after sitting on its receptor, FSH receptor, which its activation will lead to follicle To investigate whether mutations on FSH receptor are involved in POF disorder, two main inactivating mutations were The presence of two mutations 566C>T and 1555C>A were analyzed in a case control study comprised of 40 POF patients and 40 control samples which all were Iranian women who had referred to Royan Institute. Firstly their karyotype and FMR1 gene were checked to be normal. At the second stage, the DNA of peripheral blood samples was amplified by two pair of primers. For determining allelic variant status, RFLP, SSCP and Sequencing were done on the amplified PCR products. Results: All the control and case samples had the normal GCA and CCC genotype; hence no inactivating mutations (GTA & ACC respectively) were seen in Iranian POF patients. The FSH mean in the blood test of these patients were 52.5 IU/ml. Conclusion: Although these mutations, especially 566C>T were seen in other populations, this study showed that FSHR gene inactivating mutations are not frequent in Iranian POF patients. We suggest studying other inactivating mutations and polymorphisms of FSHR gene in Iranian POF patients maturation. [ABSTRACT FROM AUTHOR]