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Melanocortin receptor (MC4R) gene has been known as a candidate gene for growth traits in livestock. This research was aimed to identify the polymorphism of MC4R gene and its association with growth traits in Sumba Ongole (SO) cattle. The growth traits data consist of body weight and body size. Eighty four blood samples were collected and used for DNA isolation. The results showed one SNP (g.1133C>G) resulting in two alleles (C and G) and three genotypes (CC, CG, and GG) in SO cattle. The frequency of G allele (0.59) was higher than C allele (0.41). The CG genotype had the highest value of genotype frequency (0.48). The allele and genotype distributions followed the Hardy-Weinberg equilibrium. The single nucleotide polymorphism (SNP) g.1133C > G revealed having no significant association with growth traits in SO cattle. In conclusion, the SNP g.1133C > G may not useful as selection tool in Sumba Ongole cattle. It can be suggested that the future study is needed to find out the effect of the MC4R gene in Sumba Ongole cattle in different locus. [ABSTRACT FROM AUTHOR]

Calpastatin gene has been known as a candidate gene for meat quality in cattle, sheep, and chicken. The purpose of this study was to identify CAST gene polymorphisms and its association with meat traits in Boerka goat. The data of pH, cooking loss (CL), warner-bratzler shear force (WBSF), water holding capacity (WHC), cholesterol, water, ash, fat, and protein contains were recorded. Sequencing of 21 samples revealed five polymorphisms of CAST gene in intron 12 within Boerka goat, namely g.146C > A, g.224A > G, g.281G > A, g.737C > T, and g.431G > A. Only single nucleotide polymorphism (SNP) g.281G > A was used for genotyping. The genotype and allele frequency based on g.281G > A showed 14.29% (GG genotype) and 85.71% (GA genotype) followed with 57% G allele and 43% A allele. The chi-square test showed deviation from HWE (P<0.05) in Boerka goat. The SNP g.281G > A revealed having significantly effect to CL. The GA animals had lower CL percentage compared to the GG animals. In conclusion, the SNP selected may be used for identify meat having low CL in Boerka goat. [ABSTRACT FROM AUTHOR]

The aim of this study was to identify the polymorphism of leptin gene and its association with growth traits in Kebumen Ongole Grade cattle. One hundred blood samples were collected for molecular analysis. Polymorphism of the leptin gene was analyzed using Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR-RFLP) with HpyCH4V restriction enzyme and DNA sequencing. Association analysis of the leptin gene with growth traits was analyzed by T-test. The results showed that SNP g. 1180 C>T was found in the population. The SNP changed amino acid from arginine to cysteine. The SNP was significantly associated with a high chest circumference at weaning age in animal having CC genotype (P<0.05). There were two identified alleles, namely C and T, with frequencies were 0.885 and 0.115, respectively. The genotype frequencies of CC, CT and TT were 0.78, 0.21 and 0.01, respectively. Allelic and genotypic distribution in the studied population were in Hardy- Weinberg equilibrium. Animals with CC genotype had a higher circumference at weaning age (WCC) than those with CT genotype. In conclusion, SNP g. 1180 C> T in the leptin gene is potential as genetic marker for growth traits in Kebumen Ongole Grade cattle. [ABSTRACT FROM AUTHOR]

Abstract: The aim of this research was to assess the SRY-gene polymorphism in Madura cattle populations and the hybrids of Madura cow with Limousin bull. We investigated the specific markers of the paternally transmitted Y-chromosome for the direct detection of male-mediated introgression. We performed polymerase chain reaction and restriction fragment length polymorphisme (PCR– RFLP) to identify the polymorphism of Y-chromosomes in 17 Madura cattle from Pamekasan district, 4 conserved Madura cattle from Sapudi Island and 22 Limousin x Madura crossbred cattle from Pamekasan. A 211bp PCR product were cut with PstI and BfaI restriction enzyme. The result showed all the Madura cattle types were identified by PstI resrtiction enzyme performed into two fragments DNA. The polymorphisme of Y-chromosome was recognized by BfaI restriction enzymes at the position 432bp and 544bp. Using BfaI restriction enzyme, this study identified 68.18% of Limousin x Madura crossbred cattle was in the sequence of Y-chromosome. The high numbers of BfaI site restriction in the sequence of Y-chromosome due to the introduction Limousin bull by artificial insemination. For those, the preservation of pure Madura cattle as one of animal genetics resources in Indonesia should be a concern. [Copyright &y& Elsevier]