1. Real time, high resolution video imaging of apoptosis in single cells with a polymeric nanoprobe.
- Author
-
Lee S, Choi KY, Chung H, Ryu JH, Lee A, Koo H, Youn IC, Park JH, Kim IS, Kim SY, Chen X, Jeong SY, Kwon IC, Kim K, and Choi K
- Subjects
- Animals, Antineoplastic Agents pharmacology, Caspase 3 chemistry, Caspase 3 metabolism, Doxorubicin pharmacology, Fluorescent Dyes administration & dosage, Humans, Luminescent Measurements, Mice, Molecular Structure, Nanoparticles administration & dosage, Neoplasms, Experimental, Particle Size, Polymers administration & dosage, Surface Properties, Tumor Cells, Cultured, Apoptosis drug effects, Fluorescent Dyes chemistry, Molecular Imaging, Nanoparticles chemistry, Polymers chemistry
- Abstract
We report a new apoptosis nanoprobe (Apo-NP) designed on the basis of a polymer nanoparticle platform. This simple one-step technique is capable of boosting fluorescence signals upon apoptosis in living cells, enabling real-time imaging of apoptosis in single cells and in vivo. The Apo-NP efficiently delivers chemically labeled, dual-quenched caspase-3-sensitive fluorogenic peptides into cells, allowing caspase-3-dependent strong fluorescence amplification to be imaged in apoptotic cells in real-time and at high resolution. The design platform of the Apo-NP is flexible and can be fine-tuned for a wide array of applications such as identification of caspase-related apoptosis in pathologies and for monitoring therapeutic efficacy of apoptotic drugs in cancer treatment.
- Published
- 2011
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