5 results on '"Abati, Andrea"'
Search Results
2. Utilization of polymerase chain reaction on archival cytologic material: a comparison with fresh material with special emphasis on cerebrospinal fluids.
- Author
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Mattu R, Sorbara L, Filie AC, Little R, Wilson W, Raffeld M, and Abati A
- Subjects
- B-Lymphocytes cytology, B-Lymphocytes metabolism, Biopsy, Fine-Needle, Cell Line, Tumor, Cerebrospinal Fluid, Clone Cells metabolism, DNA, Viral cerebrospinal fluid, DNA, Viral genetics, DNA, Viral isolation & purification, Gene Rearrangement, B-Lymphocyte genetics, Gene Rearrangement, T-Lymphocyte genetics, Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor genetics, Herpesvirus 4, Human genetics, Herpesvirus 8, Human genetics, Humans, Immunoglobulin Heavy Chains genetics, Pleural Effusion, T-Lymphocytes cytology, T-Lymphocytes metabolism, Vaginal Smears, Cytodiagnosis methods, Molecular Diagnostic Techniques methods, Polymerase Chain Reaction methods
- Abstract
Use of the polymerase chain reaction (PCR) for the detection of B- and T-cell clonality, Epstein-Barr virus (EBV) and Human Herpes Virus 8 (HHV 8) infection is gaining increasing importance as a diagnostic modality. These tests are usually performed on fresh specimens. There are instances when fresh material is not available and there is a clinical utility for the performance of PCR on archival material via slide scrape lysates (SSL). However, the suitability of archival material may be questioned. Records were searched for all archival cytology cases submitted for SSL molecular diagnostics tests since 1998. Results for each case were analyzed for PCR amplification status and individual test results. A randomly chosen control group of equivalent cytologic samples submitted fresh was evaluated for comparison of amplification status. In all, 241 PCR runs were performed on SSL of archival material from 112 cytologic samples (89 cerebrospinal fluids (CSFs), 13 fine-needle aspirates (FNAs), 10 effusions). Out of these samples, 95 (85%) had amplifiable DNA, as assessed by a positive reaction for glyceraldehyde phosphate dehydrogenase (GAPDH). For the control group, 320 PCR runs were performed on 112 fresh cytologic samples (89 CSFs, 13 FNAs, 10 effusions). In total, 102 samples (91%) had amplifiable DNA. There was no statistical difference in the amplification yield between the two groups (P = 0.2177). A morphologic review of 16 of the 17 SSL archival cytologic cases that did not show amplification revealed 11/16 to be of sparse cellularity. Molecular diagnostic tests are performed routinely on fresh cytologic samples with excellent results. At times critical decisions on patient care may need to be made when fresh tissue is not available for molecular diagnostic tests. SSL of archival cytologic material can be used with excellent results for molecular diagnostic tests when fresh material is not available or when the cytologic diagnosis needs further clarification.
- Published
- 2004
- Full Text
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3. Utilization of microdissection and the polymerase chain reaction for the diagnosis of adrenal cortical carcinoma in fine-needle aspiration cytology
- Author
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Abati, Andrea, Sanjuan, Xavier, Wilder, AnnaMaria, Linehan, W. Marston, Hewitt, Steven M., and Merino, Maria J.
- Subjects
Endocrine gland cancer -- Diagnosis ,Adrenal gland diseases -- Diseases ,Polymerase chain reaction ,Health - Published
- 1999
4. Fine-needle aspiration of metastatic clear cell carcinoma of the kidney: employment of microdissection and the polymerase chain reaction as a potential diagnostic tool
- Author
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Beaty, Michael W., Zhuang, Zhengping, Park, W.S., Emmert-Buck, Michael R., Linehan, W. Marston, Lubensky, Irina A., and Abati, Andrea
- Subjects
Kidney cancer -- Diagnosis ,Biopsy, Needle ,Carcinoma, Renal cell -- Diagnosis ,Polymerase chain reaction ,Health - Abstract
BACKGROUND. The differential diagnosis of metastatic clear cell carcinoma is broad. To date, there are no specific immunohistochemical markers for renal cell carcinoma (RCC) in general use. Loss of heterozygosity (LOH) at 3p25.5, the von Hippel-Lindau (VHL) gene locus, is frequent in sporadic clear cell RCC. The authors compared LOH in primary and metastatic RCC through microdissection and the polymerase chain reaction (PCR) to evaluate these techniques as potential diagnostic tools. METHODS. The authors identified 14 patients with known clear cell RCC who underwent fine-needle aspiration (FNA) evaluation of presumed metastatic lesion. Direct-visualization microdissection was performed from archival histologic glass slides of the primary neoplasm and the adjacent normal kidney parenchyma. Malignant cell clusters were microdissected from archival FNA slides of metastatic lesions. The cytology slides were previously stained with either Diff-Quik or Papanicolaou stain. This was followed by a single-step DNA extraction and PCR amplification for evaluation of LOH using polymorphic markers, D3S1038 and D3S1110, flanking the VHL gene. RESULTS. Thirteen of the 14 cases contained DNA suitable for PCR in both the paraffin embedded and the FNA material. Eight of the 13 cases were heterozygous (informative) for the above markers, and 6 of these showed identical allelic loss in the primary and metastatic tumor for either one or both of the markers used. The remaining two cases did not show LOH at the VHL locus with the two polymorphic markers used. CONCLUSIONS. DNA from archival cytologic material stained with Papanicolaou stain or Diff-Quik is reliable for PCR amplification. Visually directed microdissection in combination with PCR has the potential to be a useful technique for confirmatory identification and diagnosis of metastatic clear cell RCC in cytologic material, because a specific genetic abnormality is present in the primary tumor. As characteristic genetic abnormalities are identified in various neoplasms, the use of this technique has the potential for conclusive evaluation of metastatic disease with FNA material, when used in comparison with surgical or cytologic material from the primary tumor. The utility of this combination of techniques has the potential for the molecular diagnosis of morphologically ambiguous cell populations. Cancer (Cancer Cyto pathol) 1997;81:180-6. [C] 1997 American Cancer Society.
- Published
- 1997
5. Sequential gene profiling of basal cell carcinomas treated with imiquimod in a placebo-controlled study defines the requirements for tissue rejection
- Author
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Panelli, Monica C, Stashower, Mitchell E, Slade, Herbert B, Smith, Kina, Norwood, Christopher, Abati, Andrea, Fetsch, Patricia, Filie, Armando, Walters, Shelley-Ann, Astry, Calvin, Aricó, Eleonora, Zhao, Yingdong, Selleri, Silvia, Wang, Ena, and Marincola, Francesco M
- Subjects
Imiquimod ,Research ,CD8 Antigens ,Gene Expression Profiling ,Interferon-alpha ,Antineoplastic Agents ,Polymerase Chain Reaction ,CD56 Antigen ,Gene Expression Regulation, Neoplastic ,Placebos ,Interferon-gamma ,Carcinoma, Basal Cell ,Aminoquinolines ,Humans ,RNA, Messenger ,Genes, Neoplasm - Abstract
An analysis of basal cell carcinoma subjected to local application of imiquimod revealed that most transcripts stimulated by imiquimod involve the activation of cellular innate and adaptive immune-effector mechanisms., Background Imiquimod is a Toll-like receptor-7 agonist capable of inducing complete clearance of basal cell carcinoma (BCC) and other cutaneous malignancies. We hypothesized that the characterization of the early transcriptional events induced by imiquimod may provide insights about immunological events preceding acute tissue and/or tumor rejection. Results We report a paired analysis of adjacent punch biopsies obtained pre- and post-treatment from 36 patients with BCC subjected to local application of imiquimod (n = 22) or vehicle cream (n = 14) in a blinded, randomized protocol. Four treatments were assessed (q12 applications for 2 or 4 days, or q24 hours for 4 or 8 days). RNA was amplified and hybridized to 17.5 K cDNA arrays. All treatment schedules similarly affected the transcriptional profile of BCC; however, the q12 × 4 days regimen, associated with highest effectiveness, induced the most changes, with 637 genes unequivocally stimulated by imiquimod. A minority of transcripts (98 genes) confirmed previous reports of interferon-α involvement. The remaining 539 genes portrayed additional immunological functions predominantly involving the activation of cellular innate and adaptive immune-effector mechanisms. Importantly, these effector signatures recapitulate previous observations of tissue rejection in the context of cancer immunotherapy, acute allograft rejection and autoimmunity. Conclusion This study, based on a powerful and reproducible model of cancer eradication by innate immune mechanisms, provides the first insights in humans into the early transcriptional events associated with immune rejection. This model is likely representative of constant immunological pathways through which innate and adaptive immune responses combine to induce tissue destruction.
- Published
- 2007
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