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Your search keyword '"Feng Liu"' showing total 4 results
Start Over Author "Feng Liu" Topic polymerase chain reaction Topic transcription factors
4 results on '"Feng Liu"'

1. Identification of coding region SNPs from specific and sensitive mRNA biomarkers for the deconvolution of the semen donor in a body fluid mixture

Author

Jinding Liu, Zidong Liu, Jie Shi, Jiangling Guo, Gengqian Zhang, Wenyan Li, Xiuying Zhang, Jiangwei Yan, Jiaqi Wang, Xiaojuan Cheng, Feng Liu, Jing Li, Jintao Li, and Ting Hao

Subjects
Forensic Genetics, Genetic Markers, Male, 0301 basic medicine, L-Iditol 2-Dehydrogenase, Saliva, Context (language use), Semen, Biology, Seminal Vesicle Secretory Proteins, Polymerase Chain Reaction, Polymorphism, Single Nucleotide, Pathology and Forensic Medicine, 03 medical and health sciences, fluids and secretions, 0302 clinical medicine, Multiplex polymerase chain reaction, Genetics, Humans, Coding region, RNA, Messenger, 030216 legal & forensic medicine, Creatine Kinase, Homeodomain Proteins, Body fluid, Transglutaminases, Electrophoresis, Capillary, Nuclear Proteins, Prostate-Specific Antigen, Molecular biology, Reverse transcription polymerase chain reaction, genomic DNA, 030104 developmental biology, Cervix Mucus, Female, Kallikreins, Multiplex Polymerase Chain Reaction, Blood Chemical Analysis, Transcription Factors
Abstract

mRNA markers provide a very promising method for the identification of human body fluids or tissues in the context of forensic investigations. Previous studies have shown that different body fluids can be distinguished from each other according to their specific mRNA biomarkers. In this study, we evaluated eight semen-specific mRNA markers (KLK3, NKX3–1, CKB, KLK2, PRAC1, SEMG1, TGM4, and SORD) that encompass 12 coding single nucleotide polymorphisms (cSNPs) to identify the semen contributor in a mixed stain. Five highly specific and sensitive mRNA markers for blood, menstrual blood, saliva, vaginal secretions, and skin were also incorporated into the PCR system as body fluid-positive controls. Reverse transcription polymerase chain reaction (RT-PCR), multiplex PCR and SNaPshot mini-sequencing assays were established for the identification of semen-specific mRNA. The amplicon size ranged from 133 to 337 bp. The semen-specific system was examined against blood, menstrual blood, saliva, vaginal secretions, and skin swabs. The eight mRNA biomarkers were semen-specific and could be successfully typed in laboratory-generated mixtures composed of different body fluids supplemented with 1 ng of semen cDNA. This system possessed a high sensitivity that ranged from 1:10–1:100 for detecting trace amounts of semen in semen-containing body fluid mixtures. Additionally, our results demonstrated that the cSNPs polymorphisms included in the mRNA markers were concordant with genomic DNA (gDNA). Despite the presence of other body fluids, the system exhibited high sensitivity and specificity to the semen in the mixture. In future studies, we will add other cSNPs from the semen-specific genes using massively parallel sequencing to further improve our system.

Published
2021

2. Cloning and expression of the Acanthamoeba castellanii gene encoding transcription factor TFIID

Author

Jiemin Wong, Feng Liu, and Erik Bateman

Subjects
TATA box, Molecular Sequence Data, genetic processes, information science, Acanthamoeba, macromolecular substances, Biology, Polymerase Chain Reaction, Escherichia coli, Genetics, Animals, Amino Acid Sequence, RNA, Messenger, Cloning, Molecular, Codon, Base Sequence, fungi, General Medicine, DNA, Protozoan, Blotting, Northern, Recombinant Proteins, Cell biology, Blotting, Southern, TAF1, TAF4, TAF2, Transcription Factor TFIID, health occupations, Acanthamoeba castellanii, Transcription factor II D, Sequence Alignment, RNA, Protozoan, Transcription factor II A, Transcription Factors
Abstract

We have cloned and characterized the cDNA encoding transcription factor TFIID from the eukaryote, Acanthamoeba castellanii. The gene occurs as a single species, encodes one mRNA and, presumably, a single protein. A. castellanii TFIID contains two recognizable domains, a nonconserved N-terminal domain and a highly conserved C-terminal domain. Similarities between the amino acid (aa) sequences of TFIID from several organisms are also found within the N-terminal 78 aa, suggesting a potential role in TFIID function. Full-length or truncated A. castellanii TFIID produced in Escherichia coli binds to a TATA box and is able to activate transcription in a TFIID-depleted HeLa cell extract, but the C-terminal 180-aa domain was found to be less efficient in these reactions.

Published
1992

3. Respiratory syncytial virus infection modulates interleukin-8 production in respiratory epithelial cells through a transcription factor-activator protein-1 signaling pathway.

Author

YAO ZHOU, JIN YANG, HUAN DENG, HONG XU, JIAMIN ZHANG, WEISONG JIN, HAIYAN GAO, FENG LIU, and DEYU ZHAO

Subjects
RESPIRATORY syncytial virus infections, INTERLEUKIN-8, EPITHELIAL cells, TRANSCRIPTION factors, MESSENGER RNA, POLYMERASE chain reaction
Abstract

Respiratory syncytial virus (RSV) is a leading cause of respiratory duct infection that can result in severe clinical symptoms, particularly among children under 3 years of age. In the current study, the effect of RSV on airway epithelial cell function and the potential signaling pathways involved were investigated. A549 human airway epithelial cells were infected with RSV at a multiplicity of infection of 1. After 24 h, interleukin (IL)-8 secretion in the cell supernatant was analyzed. A microarray assay of RSV-infected A549 cells was conducted in order to identify any potential pathways involved, and quantitative polymerase chain reaction was performed to examine mRNA expression levels in these pathways. Electrophoretic mobility shift assays of nuclear transcription factors were conducted for further verification. IL-8 levels increased significantly in the supernatant of RSV-infected A549 cells compared with levels in non-infected cells. Microarray data suggested the involvement of the Toll-like receptor 4 (TLR4) pathway, and mRNA expression levels of genes (MYD88, TRAM and TRIF) involved in this pathway were higher in infected cells. Enhanced synthesis of activator protein-1 (AP-1) was observed. RSV infection of A549 cells may promote IL-8 secretion. In conclusion, the results of the present study indicate that the TLR4 signaling pathway, in conjunction with MYD88, TRAM, TRIF and the transcription factor AP-1, may activate immune responses to RSV infection in airway epithelial cells. [ABSTRACT FROM AUTHOR]

Published
2014
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4. Genome-Wide Analysis of a TaLEA-Introduced Transgenic Populus simonii x Populus nigra Dwarf Mutant.

Author

Hong-Mei Yuan, Su Chen, Lin Lin, Rui Wei, Hui-Yu Li, Gui-Feng Liu, and Jing Jiang

Subjects
BLACK poplar, AGROBACTERIUM tumefaciens, CHROMOSOMES, TRANSCRIPTION factors, POLYMERASE chain reaction
Abstract

A dwarf mutant (dwf1) was obtained among 15 transgenic lines, when TaLEA (Tamarix androssowii late embryogenesis abundant gene) was introduced into Populus simonii x Populus nigra by Agrobacterium tumefaciens-mediated transformation. Under the same growth conditions, dwf1 height was significantly reduced compared with the wild type and the other transgenic lines. Because only one transgenic line (dwf1) displayed the dwarf phenotype, we considered that T-DNA insertion sites may play a role in the mutant formation. The mechanisms underlying this effect were investigated using TAIL-PCR (thermal asymmetric interlaced PCR) and microarrays methods. According to the TAIL-PCR results, two flanking sequences located on chromosome IV and VIII respectively, were cloned. The results indicated the integration of two independent T-DNA copies. We searched for the potential genes near to the T-DNA insertions. The nearest gene was a putative poplar AP2 transcription factor (GI: 224073210). Expression analysis showed that AP2 was up-regulated in dwf1 compared with the wild type and the other transgenic lines. According to the microarrays results, a total of 537 genes involved in hydrolase, kinase and transcription factor activities, as well as protein and nucleotide binding, showed significant alterations in gene expression. These genes were expressed in more than 60 metabolic pathways, including starch, sucrose, galactose and glycerolipid metabolism and phenylpropanoids and flavonoid biosyntheses. Our transcriptome and T-DNA insertion sites analyses might provide some useful insights into the dwarf mutant formation. [ABSTRACT FROM AUTHOR]

Published
2012
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