1. Biosynthesis of rubradirin as an ansamycin antibiotic from Streptomyces achromogenes var. rubradiris NRRL3061
- Author
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Kwangkyoung Liou, Kyoung-Wook Kim, Sung-Ho Kang, Jyoti Maharjan, Dong-Hee Kim, Jae Kyung Sohng, Tae-Sung Jeong, Van Dat Nguyen, Kwang-Il Song, Jae Seon Kang, Young-Soo Hong, Tae-Jin Oh, Chun-Gyu Kim, Janardan Lamichhane, Jung-Joon Lee, and Jin-Cheol Yoo
- Subjects
DNA, Bacterial ,Sequence analysis ,Molecular Sequence Data ,Streptomyces achromogenes ,Biochemistry ,Microbiology ,Streptomyces ,Open Reading Frames ,Polyketide synthase ,Genetics ,Hydroxybenzoates ,Aminobenzoates ,Gene Silencing ,Glycosides ,ORFS ,Molecular Biology ,biology ,Base Sequence ,Streptomycetaceae ,Ansamycin ,General Medicine ,Sequence Analysis, DNA ,biology.organism_classification ,Cosmids ,Anti-Bacterial Agents ,Open reading frame ,Multigene Family ,Fermentation ,biology.protein ,Naphthoquinones - Abstract
The four overlapping cosmids from the rubradirin producer, Streptomyces achromogenes var rubradiris NRRL 3061, have 58 ORFs within a 105.6 kb fragment. These ORFs harbored essential genes responsible for the formation and attachment of four distinct moieties, along with the genes associated with regulatory, resistance, and transport functions. The PKS (rubA) and glycosyltransferase (rubG2) genes were disrupted in order to demonstrate a complete elimination of rubradirin production. The rubradirin biosynthetic pathway was proposed based on the putative functions of the gene products, the functional identification of sugar genes, and the mutant strains.
- Published
- 2006