1. Spacer structure and hydrophobicity influences transfection activity of novel polycationic gemini amphiphiles.
- Author
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Puchkov PA, Kartashova IA, Shmendel EV, Luneva AS, Morozova NG, Zenkova MA, and Maslov MA
- Subjects
- DNA genetics, HEK293 Cells, Humans, Hydrophobic and Hydrophilic Interactions, Lipids chemistry, Liposomes chemistry, Phosphatidylethanolamines chemistry, Plasmids genetics, Polyelectrolytes, RNA, Small Interfering genetics, DNA administration & dosage, Plasmids administration & dosage, Polyamines chemistry, RNA, Small Interfering administration & dosage, Surface-Active Agents chemistry, Transfection methods
- Abstract
Three novel polycationic gemini amphiphiles with different spacers were developed and evaluated in terms of their physiochemical properties and transfection efficiencies. Cationic liposomes formed by these amphiphiles and the helper lipid DOPE were able to successfully condense DNA, as shown by gel mobility shift and ethidium bromide intercalation assays. Transfection activity of the liposomes was superior to Lipofectamine
® 2000 and was dependent on spacer structure, hydrophobicity, and nucleic acid type (pDNA or siRNA). We demonstrated that the cationic liposomes 2X6/DOPE and 2X7/DOPE are potential non-toxic vehicles for gene delivery., (Copyright © 2017 Elsevier Ltd. All rights reserved.)- Published
- 2017
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