1. Cytoplasmic ribosomal protein L14B is essential for fertilization in Arabidopsis.
- Author
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Luo A, Zhan H, Zhang X, Du H, Zhang Y, and Peng X
- Subjects
- Arabidopsis genetics, Arabidopsis Proteins metabolism, Cytoplasm, Pollen genetics, Pollen Tube genetics, Pollen Tube growth & development, Ribosomal Proteins deficiency, Ribosomal Proteins metabolism, Arabidopsis physiology, Arabidopsis Proteins genetics, Fertilization, Pollen anatomy & histology, Pollen Tube physiology, Ribosomal Proteins genetics
- Abstract
Plant cytoplasmic ribosomal proteins not only participate in protein synthesis, but also have specific roles in developmental regulation. However, the high heterogeneity of plant ribosome makes our understanding of these proteins very limited. Here we reported that RPL14B, a component of the ribosome large subunit, is critical for fertilization in Arabidopsis. RPL14B is existed in a majority of organs and tissues. No homozygous rpl14b mutant is available, indicating that RPL14B is irreplaceable for sexual reproduction. Smaller-sized rpl14b pollens could germinate normally, but pollen tube competitiveness is grievously weakened. Beside, cell fate specification is impaired in female gametophytes from heterozygous rpl14b/RPL14B ovules, resulting in defect of micropylar pollen tube attraction. However, this defect could be restored by restricted expression of RPL14B in synergid cells. Successful fertilization requires normal pollen tube growth and precise pollen tube guidance. Thus our results show a novel role of RPL14B in fertilization and shed new light on regulatory mechanism of pollen tube growth and precise pollen tube guidance., Competing Interests: Declaration of Competing Interest There are no conflicts to declare., (Copyright © 2020 Elsevier B.V. All rights reserved.)
- Published
- 2020
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