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Kentucky bluegrass (Poa pratensis L.) has become dominant in many portions of the northern Great Plains. During hot and/or dry periods, pastures consisting of mostly cool‐season grasses will most likely lack the productivity and diversity needed to provide sufficient forage for livestock. Determining the impact of weather conditions on the variation of forage production and nutritive response of Kentucky bluegrass is becoming increasingly important to agricultural producers. Sampling occurred at the USDA Northern Great Plains Research Laboratory near Mandan, North Dakota, USA. Kentucky bluegrass samples were clipped every 2–3 weeks in mowed and unmowed unfertilized rangeland plots during the growing seasons of 2017 and 2018. Samples were dried, weighed, ground to pass a 1‐mm sieve, and analysed for nutritive value. There are differences between the modelled supply of metabolizable energy and metabolizable protein and the requirements for cow‐calf pairs for all years and most months. Early defoliation of Kentucky bluegrass decreases productivity and nutritive value as the grazing season progresses. Adjusting management to allow an adequate growth interval and plant canopy cover throughout the year can ensure a more consistent supply of Kentucky bluegrass. Our data and models provide a glimpse into future scenarios, which allow producers to be more proactive in dealing with Kentucky bluegrass and projected changes in climate. [ABSTRACT FROM AUTHOR]

Recent advances in irrigation technologies have led many states to incentivize homeowners to purchase United States Environmental Protection Agency WaterSense-labeled, smart irrigation controllers. However, previous research of smart controllers has shown that their use may still result in excess water application when compared with controllers manually programmed to replace actual water loss. This study compared kentucky bluegrass (Poa pratensis) irrigation applications using three smart irrigation controllers, a conventional irrigation controller programmed according to Cooperative Extension recommendations, and the average irrigation rate of area homeowners in Utah during 2018 and 2019. Of all the controllers tested, the manually programmed controller applied water at amounts closest to the actual evapotranspiration rates; however, smart controllers applied from 30% to 63% less water than area homeowners, depending on the controller and year of the study. Kentucky bluegrass health and quality indicators—percent green cover and normalized difference vegetation indices—varied between years of the study and were lower than acceptable levels on several occasions in 2019 for three of the four controllers tested. Compared with the results of similar studies, these findings suggest that the effects of smart irrigation controllers on turfgrass health and quality may vary by location and over time.

The field experiment was conducted in Poland between 2013 and 2015. The research was carried out in the split plot design with three replications. The following grass species were used in the experiment (factor B): Lolium perenne, Festuca rubra and Poa pratensis. Another experimental factor tested in the research was soil conditioners (factor A) as Substral (S), Humus Active Papka (HAP), Eko- Użyźniacz (EU), UGmax (UG). In each year of the experiment, among other things, grass growth, i.e. its slow rate of growth, was assessed. It was based on measurements of the height of plants before harvest. A 9-point scale was used. This assessment was conducted in three seasons (spring, summer, autumn). In all seasons red fescue had the most desirable intensity of growth (the slowest) and permanent ryegrass the fastest, both determined as an average effect of all soil conditioners. Of the applied soil conditioners, lawns treated with Humus Active Papka, grew the most slowly, taken as an average for all grass species and for all seasons. Of all grass species tested in the experiment red fescue had the smallest variation of growth intensity during the research. [ABSTRACT FROM AUTHOR]

Kentucky bluegrass (Poa pratensis L.) is a highly adapted and important turfgrass species in cool-season climates. It has high and variable polyploidy, small and metacentric chromosomes, and a facultative apomictic breeding system. As a result of the polyploidy and apomixis, identifying hybrids for Mendelian selection, identifying fixed apomictic progeny of desirable hybridizations for cultivar development, or differentiating among cultivars with subtle phenotypic differences is challenging without the assistance of molecular markers. Herein, we show data and review previous research showing the uses and limitations of using molecular markers for hybrid detection, apomixis assessment, and cultivar discrimination. In order to differentiate among different apomictic offtypes, both molecular markers and flow cytometry are necessary. For assessing similarity among progeny of hybridizations, as well as discriminating among cultivars, sets of markers are necessary and cryptic molecular variation must be considered. High throughput genotyping platforms are critical for increased genotyping efficiency. [ABSTRACT FROM AUTHOR]

Turf managers are continually seeking improved grasses, management practices, and products that enhance heat and drought tolerance and reduce supplemental irrigation needs. To this end, products like seaweed extract (SWE) have been extensively studied on short-cut (≤12 mm) golf turf and seedlings of various turfgrass species exposed to stress conditions. Few studies, however, have reported SWE effects on mature, higher cut (≥38 mm) cool-season turfgrass swards. A 3-year field study (2013-15) was conducted in Connecticut to determine the effect of various SWE treatments on the normalized difference vegetative index (NDVI) response of nonirrigated kentucky bluegrass (Poa pratensis L.) and tall fescue (Festuca arundinacea Schreb.) turf managed as a lawn and cut at 76.2 mm. Separate experiments for each species were set out as randomized complete block designs with three replicates. Throughout the growing season in each year, various liquid SWEs were applied at a concentration of 9.55 L·ha-1 weekly or 19.1 L·ha-1 biweekly. A nontreated control was included. The study lacked extreme heat stress conditions during the yearly growing seasons, but periodic moisture deficits below normal were present. Within each year, there were no significant SWE effects on the NDVI of either species. The results suggest that there is no improvement in the NDVI by applying SWEs to mature, higher cut cool-season turfgrass lawns under less than extreme heat-stress conditions, water-stress conditions, or both. Because this study was conducted only at one site without extreme stress, further research of SWE applications to established, higher cut cool-season turfgrass lawns should be conducted across different locations and soils to determine the effects of applying SWE to these stands under extreme heat-stress conditions, water-stress conditions, or both. [ABSTRACT FROM AUTHOR]

Background: Poa pratensis L. is a turf grass and forage crop used worldwide. Being a facultative apomictic species, P. pratensis has a highly variable chromosome number. Chromosomal markers constitute a powerful tool for chromosome identification and for various aspects of genomic research. However, currently, no chromosomal markers are available for P. pratensis. Results: Four novel chromosome markers were isolated from a screen of Cot-1 DNA libraries, combined with fluorescence in situ hybridization (FISH) in Poa pratensis. Three tandemly repetitive sequences (PpTR-1, PpTR-2, and PpTR-3) were characterized as subtelomeric. Monomers of 318 bp, 189 bp and 189 bp were identified in PpTR-1, PpTR-2, and PpTR-3, respectively. One tandemly repetitive sequence (PpCR-1) was shown to be centromeric or pericentromeric, and it had a monomer of 27 bp. The distribution patterns of PpTR-1, PpTR-2, and PpTR-3 were highly conserved across different P. pratensis cultivars and in the distantly related Poa species, whereas PpCR-1 was conserved across different P. pratensis cultivars, but less conserved across Poa species. Conclusion: In this study, we report the identification and characterization of four novel chromosomal markers in P. pratensis. These chromosomal markers are powerful tools for accurate assessment of chromosome count, genomic and phylogenetic analyses, as well as studies of apomixis in P. pratensis. [ABSTRACT FROM AUTHOR]

ABSTRACT Grasslands of the Northern Great Plains of North America are in the midst of extensive human‐driven loss and redistribution of important species. Invasive plants contribute to degradation of this ecosystem and present monumental challenges for natural resource managers. Widespread decreases in populations of grassland‐dependent wildlife, particularly birds, have been documented, but relatively little research has been focused on direct responses of wildlife to invading plants relative to native vegetation. Smooth brome (Bromus inermis) and Kentucky bluegrass (Poa pratensis) are 2 invasive cool‐season grass species found across much of the Northern Great Plains that continue to expand despite management actions intended to reduce them. With eradication unlikely, creativity, cooperation, and science‐based management (e.g., Adaptive Management) are paramount to protect and restore the integrity of grassland habitats across the Northern Great Plains. Published 2013. This article is a U.S. Government work and is in the public domain in the USA.

Background: The cuticular wax plays important roles in plant resistance to various biotic and abiotic stresses. Understanding the synthesis and secretion of cuticular waxes is necessary in utilizing cuticular waxes to improve crop productivity and plant ecological adaptation. Due to the lack of genomic resources, little genetic research on cuticular wax deposition has been focused on Poa pratensis, a perennial forage and turf grass species that is widely distributed under various habitats. In this study, we performed de novo transcriptome sequencing to explore differentially expressed genes between the leaf non-elongation zone (NEZm) and the emerged blade zone (EBZ) and to identify genes related to cuticular wax deposition. Results: A total of 77,707,414 high quality reads were obtained from llumina HiSeq 2500 platform, which were then assembled into 106,766 unigenes. Among them, 6019 unigenes showed significant differences in expression between NEZm and EBZ. In our assembled sequences, 3087 SSRs molecular markers were discovered. All the unigenes were searched against the NR, Swissprot, GO, COG, and KEGG databases using BLAST program for functional annotation. From 3156 unigenes with more expression in NEZm compared to EBZ, a number of unigenes involved in very long chain fatty acids (VLCFAs) and cuticular wax biosynthesis, transportation and regulation were identified. Several unigenes related to defense response and epidermal patterning were also found. Twelve putative genes involved in VLCFAs and cuticular wax biosynthesis were further analyzed for their expressions using qRT-PCR. Conclusions: The transcriptome of P. pratensis leaf was deep sequenced, de novo assembled and annotated, and the candidate genes potentially involved in VLCFAs and cuticular wax biosynthesis, secretion and regulation in P. pratensis were identified. This provides fundamental genetic resources in improving plant adaptation to abiotic and biotic stresses. [ABSTRACT FROM AUTHOR]

The recent expansion of deciduous shrubs is a common observation throughout the Arctic. However, we lack a complete understanding of how physiological differences between deciduous shrubs and coexisting species may confer competitive advantages to shrubs. We combined leaf gas exchange and stable isotope analyses of two important species, Betula nana and Poa pratensis, to elucidate the processes governing seasonal carbon (C) gain in West Greenland. We tested two competing hypotheses. On one hand, we anticipated the cooler, drier soils beneath the Betula canopy could result in greater drought sensitivity in this species. Alternatively, because Poa tends to occupy sites with wetter soils, we hypothesized that it may be more sensitive to drought. Our results revealed greater drought sensitivity in Poa, which displayed reductions in Amax and gs during periods of high atmospheric demand and dry soils. Additionally, leaf Δ13C and Δ18O were negatively correlated in Poa, suggesting strong stomatal influence on Δ13C. Conversely, there was no relationship between gs and canopy or soil microclimates and no correlation between leaf Δ13C and Δ18O in Betula, indicating that variation in Δ13C may have been driven by variation in photosynthesis. Our results suggest that Poa is more susceptible to drought than Betula, whereas Betula was able to maintain steady, yet conservative, C gain. These differences in C-H2O relations may confer a competitive advantage to Betula in a warmer, drier climate. [ABSTRACT FROM AUTHOR]

Phleum alpinum L. and Poa pratensis L. are major forage species that often grow in various environments in Tierra del Fuego, Argentina. We performed a greenhouse experiment to investigate how these species acclimate to the different irradiance of the microenvironments where they grow. Both grass species were exposed to three levels of incident irradiance (I4: 4%; I26: 26%, or I64: 64% of ambient sunlight) and two levels of soil moisture content (M30: 30-50% or M60: 60-80% of field capacity) under greenhouse conditions. As irradiance levels increased, the contents of chlorophyll per unit surface area and fresh weight basis increased, and the chlorophyll a/ b and carotenoids/chlorophyll ratio also increased. Maximum photosynthetic rate and the light compensation point increased with increasing light availability. Values for these variables varied with time. However, the relationship of these values was not modified in P. alpinum between the irradiance treatments. Contrarily, temporal changes of those variables showed that the maximum photosynthetic rate was similar to that in March in all treatments in P. pratensis. Results indicated that P. alpinum and P. pratensis were able to acclimate to the various experimental environments. [ABSTRACT FROM AUTHOR]

Background: Kentucky bluegrass (Poa pratensis L.) is a prominent turfgrass in the cool-season regions, but it is sensitive to salt stress. Previously, a relatively salt tolerant Kentucky bluegrass accession was identified that maintained green colour under consistent salt applications. In this study, a transcriptome study between the tolerant (PI 372742) accession and a salt susceptible (PI 368233) accession was conducted, under control and salt treatments, and in shoot and root tissues. Results: Sample replicates grouped tightly by tissue and treatment, and fewer differentially expressed transcripts were detected in the tolerant PI 372742 samples compared to the susceptible PI 368233 samples, and in root tissues compared to shoot tissues. A de novo assembly resulted in 388,764 transcripts, with 36,587 detected as differentially expressed. Approximately 75 % of transcripts had homology based annotations, with several differences in GO terms enriched between the PI 368233 and PI 372742 samples. Gene expression profiling identified salt-responsive gene families that were consistently down-regulated in PI 372742 and unlikely to contribute to salt tolerance in Kentucky bluegrass. Gene expression profiling also identified sets of transcripts relating to transcription factors, ion and water transport genes, and oxidation-reduction process genes with likely roles in salt tolerance. Conclusions: The transcript assembly represents the first such assembly in the highly polyploidy, facultative apomictic Kentucky bluegrass. The transcripts identified provide genetic information on how this plant responds to and tolerates salt stress in both shoot and root tissues, and can be used for further genetic testing and introgression. [ABSTRACT FROM AUTHOR]

A common complaint of ecological restoration is there have been few systematic analyses of past restorations which has led to a lack of evidence informing future restorations. An analysis was done on 123 past native grassland reconstructions, spanning the last 40 years, in eastern North Dakota and northwestern Minnesota. A cluster analysis and indicator species analysis were used to place the 123 surveyed reconstructions into five significantly different groups. The five groups were separated into a Native group with the sub-groups, Big Bluestem Dominated (BBD) and Native Grass Mix (NGM) having native species as indicators, and an Exotic group with two exotic grasses, smooth brome (Bromus inermis) and Kentucky bluegrass (Poa pratensis), being indicators. Thirty-eight percent of the reconstructions were classified as Exotic. A logistic regression was used to assign membership probability to the Native group based on: 1) seed mix species richness; 2) number of grass species in the seed mix; 3) percent of grasses in seed mix; 4) time of year when seeded; and 5) seeding method. The probability of membership to the Native group for dormant season seeding, broadcast seeding, and 20+ species natives seed mix was 100%, 74%, and 81%, respectively. The probabilities of the different practices in relation to the reconstruction outcomes will provide evidence for managers on how to plan for future reconstructions. [ABSTRACT FROM AUTHOR]

Rusts are destructive fungal diseases that can cause severe thinning and unattractive discoloration of kentucky bluegrass (KBG; Poa pratensis L.). Currently, turfgrass breeding programs rely on field evaluations to screen KBG germplasm for rust resistance; methods that are expensive, labor intensive, and require large turf areas. The availability of a greenhouse-based assay to perform prescreening of KBG germplasm for resistance to rust diseases before field trials would allow breeders to remove the poorest performing plants before field evaluations thus enhancing breeding efficiency. In this study, we set out to develop a reliable, low-cost greenhouse inoculation protocol for the two most common rust pathogens of KBG in temperate growing regions: Puccinia coronata and Puccinia graminis, the causal agents of crown and stem rust, respectively. Using a modified inoculation assay and custom-built plexi-glass chambers adapted from protocols used for cereal rust pathogens, urediniospores of crown and stem rust fungi developed on inoculated plants 10 to 14 days postinoculation. Real-time polymerase chain reaction (PCR) assays, disease symptomology, and morphology of urediniospores confirmed the presence and identity of both rust pathogens from inoculated host tissue. The inoculation protocols described here represent an effective method to accelerate screening of KBG germplasm for resistance to crown and stem rust diseases. Infection of KBG plants in the greenhouse will also allow breeders to maintain populations of crown and stem rust fungi throughout the year, providing a reliable and ongoing source of pathogen inoculum for experimentation and screening in the future. [ABSTRACT FROM AUTHOR]

Adequate turfgrass sod strength for harvesting and handling is typically determined by the producer's past experience and subjective appraisal. This study was conducted to determine the relationship between producer subjective sod-strength assessments and quantitative shear-strength measurements with predominantly kentucky bluegrass (Poa pratensis L.) turf. Across three consecutive growing seasons, 93 samples were collected from sod fields in Rhode Island and assessed for sod strength by subjective and quantitative methods. Producer subjective ratings of sod strength were significantly (P < 0.0001) associated with quantitative measurements of peak force required to shear a sod strip. Minimally acceptable strength occurred most frequently when peak shear force was between 55 and 85 kg⋅m-1 width of sod; whereas preferred sod strength occurred most frequently when peak shear force was between 70 and 140 kg⋅m-1 width of sod. Once peak force exceeded 58 and 86 kg⋅m-1, there was a > 50% probability that sod strength would be judged at least adequate and at preferred strength, respectively, up to a peak force of 140 kg⋅m-1. The results suggest that quantitative measurements of shear strength can be related to producer subjective assessments, and provide unbiased benchmark values to guide management decisions for kentucky bluegrass sod production. [ABSTRACT FROM AUTHOR]

Measurements were taken of seven morphological characteristics of various forms of Poa pratensis, including ecotypes (12), cultivars (4), and breeding lines (2). Statistically significant differences relating to the average number and length of vegetative shoots and the leaf width of the forms studied were determined using Tukey's HSD test. Evaluation of genetic polymorphism was carried out using the RAPD-PCR method. 18 primers were used for molecular analysis and 473 amplification products were obtained, in which high levels of polymorphism (84.2%) and specificity (63) were discovered. The mean value of Dice's coefficient, the genetic similarity indicator, was about 0.7. These results indicate a significant genetic similarity between most of the forms of Poa pratensis studied. A correlation was also observed between genetic variation and phenotypic diversity of the studied forms of Poa pratensis. Convergence was found to occur between phenotype and genetic similarity among the forms. Only the dos01 468 ecotype showed discrepancies in the assessment of phenotypic and genomic variability. [ABSTRACT FROM AUTHOR]

In Isle Royale National Park, heavy moose browsing over the past 100 years has suppressed the regeneration of many tree species, gradually resulting in a shift towards more open forests and savannas. By 1996, 16% of the forests at the southwestern end of Isle Royale had become savanna and another 20% of forests were starting to have canopy breakup. The changes in understory vegetation brought about by savanna formation have received little attention, even though the future of moose and wolf populations on Isle Royale is tied to these vegetation changes. This study examined the vegetation of savannas ranging in age (date since formation) from <10 years to 80 years to examine how the ground flora changes over time from initial forest canopy breakup, to extensive grassland, and finally to a zootic subclimax dubbed 'moose-spruce savanna.' Ordination techniques were used to describe plant communities and to identify environmental variables that influence vegetation development. Nonparametric multiplicative regression was used to predict how these variables influenced individual plants and structure within the communities. Plant communities had a notable shift from forest herbs to ruderal species, especially non-native plants. This succession was influenced strongly by the underlying bedrock and hydrology-altering moisture regimes and plant communities. Picea glauca (Moench) Voss. and Poa pratensis L. competed in a dynamic inhibitory relationship. The former facilitated forest plants while the latter out-competed other plants and promoted open swards. These interactions are dramatically changing the character of Isle Royale's upland plant communities and will have important trophic consequences for the island. [ABSTRACT FROM AUTHOR]

As a commonly used turfgrass, Kentucky bluegrass (Poa pratensis L.) (KBG) has many commercially available cultivars for production. After several years of screening, two new lines were obtained ('KBG03' and 'KBG04'), which have high tolerance to summer. The study showed that the two lines revealed similar morphological characteristics, with light green leaf color, narrow leaf blade, high plant height and light 1,0007grain weight. A total of 400 random amplified polymorphic DNA (RAPD) primers and 256 sequence7related amplified polymorphism (SRAP) primer combinations were screened among the two lines and other 4 imported commercial cultivars. The percentages of polymorphic sites were 65.5% (RAPD) and 22.6% (SRAP) respectively. By cluster analysis of RAPD and SRAP data, the dendrogram at a similarity of 0.29 gave two main clusters, of which one group had 4 commercial cultivars, and the other had the two new breeding lines. Furthermore, one specific band of 'KBG04' was successfully converted into a dominant sequence characterized amplified region marker (SCAR196). Then the SCAR marker was verified by 39 KBG DNA samples, including imported varieties, domestic varieties and self7breeding lines of our laboratory, and it exhibited high consistency with the original RAPD polymorphic amplification. The results showed that the SCAR marker can be used to distinguish the new line 'KBG04' from numerous KBG germplasms, which would be useful for cultivar identification and property rights protection in the future. [ABSTRACT FROM AUTHOR]

Phytoliths in Poa pratensis L. (Poaceae) leaf blades and sheaths are described in this study. The role of plant opal particles--known as phytoliths--is considerable in taxonomical studies, and their long-term preservation in sediments makes them a useful tool in the reconstruction of ancient plant communities and plant-human interactions. All together, 2244 phytoliths were counted and analyzed in 25 plant samples (5 shoots of 5 specimens and approximately 500-600 phytoliths per specimen). The biogenic silica content of P. pratensis leaves was determined at 2.61%, and 27 morphotypes have been described using the International Code for Phytolith Nomenclature. Two morphotypes are described for the first time in this study. Long cells (elongate psilate and sinuate morphotypes) and short cells (rondel-trapeziform elongated and rounded morphotypes) are frequently present in this species. Differences in morphotype frequency and significant differences in a few simple morphometric data (length, width, height) of long cells and short cells were found among specimens, which suggests that these features vary depending on environmental factors and the maturity of leaf tissues. [ABSTRACT FROM AUTHOR]

Aims Invasive plants commonly occupy disturbed soils, thereby providing a stage for understanding the role of disturbance-enhanced resources in plant invasions. Here, we addressed how soil space and soil nutrients affect the growth and competitive effect of invasive plants and whether this effect varies with different invaders. Methods We conducted an experiment in which two invasive plants (Bromus tectorum and Centaurea maculosa) and one native species (Poa pratensis) were grown alone or together in four habitats consisting of two levels of soil space and nutrients. At the end of the experiment, we determined the total biomass, biomass allocation and relative interaction intensity of B. tectorum, C. maculosa and P. pratensis. Important Findings Across two invaders, B. tectorum and C. maculosa, increased soil nutrients had greater positive effects on their growth than increased soil space, the effects of soil space on root weight ratio were greater than those of soil nutrients, and their competitive effect decreased with soil space but increased with soil nutrients. These findings suggest that changing soil space and nutrients differentially influence the growth and competitive advantages of two invaders. Bromus tectorum benefited more from increased soil resources than C. maculosa. Soil space and nutrients affected the biomass allocation of C. maculosa but not B. tectorum. The competitive effect of B. tectorum was unaffected by soil space and soil nutrients, but the opposite was the case for C. maculosa. Thus, the effects of soil space and nutrients on growth and competitive ability depend on invasive species identity. [ABSTRACT FROM AUTHOR]

In addition to typical meadow species, Poa pratensis, 4 other species are recognized within this aggregate. Only the leaf width is used often for their identification. These species are known to be satisfactorily distinguished in their growth habits, but detailed studies showed that growth habit is not a reliable discriminator and it seems to be only a reflection of general trends. Neither width of leaves, nor characters of growth habit are successful features in their identification. To avoid wrong identifications, which could lead undesirable consequences, all morphological characters and growth habit should be simultaneously taken into consideration. [ABSTRACT FROM AUTHOR]

ABSTRACT Grasslands of the Northern Great Plains of North America are in the midst of extensive human-driven loss and redistribution of important species. Invasive plants contribute to degradation of this ecosystem and present monumental challenges for natural resource managers. Widespread decreases in populations of grassland-dependent wildlife, particularly birds, have been documented, but relatively little research has been focused on direct responses of wildlife to invading plants relative to native vegetation. Smooth brome ( Bromus inermis) and Kentucky bluegrass ( Poa pratensis) are 2 invasive cool-season grass species found across much of the Northern Great Plains that continue to expand despite management actions intended to reduce them. With eradication unlikely, creativity, cooperation, and science-based management (e.g., Adaptive Management) are paramount to protect and restore the integrity of grassland habitats across the Northern Great Plains. Published 2013. This article is a U.S. Government work and is in the public domain in the USA. [ABSTRACT FROM AUTHOR]

Global climate change will likely result in the reduction of water levels in intermountain wetlands and ponds, and the vegetation communities associated with these wetlands are an important forage source for livestock. Lowered water levels will not only constrict wetland plant communities, it will potentially change aquatic and soil salt concentrations. Such an increase in salinity can reduce plant growth and potentially affect competitive interactions between plants. A greenhouse experiment examined the effects of salinity and competition on the growth of two wet meadow grass species, Poa pratensis (a glycophyte) and Puccinellia nuttalliana (a halophyte). The following hypotheses based on published data were tested: (1) Biomass of both species will decrease with increasing concentration of salt; (2) root:shoot (R:S) ratio of P. pratensis will decrease with increasing salt concentration while R:S ratio of P. pratensis and P. nuttalliana will increase with clipping; (3) competitive importance will decrease for P. pratensis and P. nuttalliana with increasing salt concentration because salt induces a stress response and competitive importance is reduced in stressed environments. A factorial design included 3 plant treatments ( P. nuttalliana alone, P. pratentsis alone, P. nuttalliana + P. pratensis) × 4 salinity rates (control; 5, 10, 15 g/L NaCl) × 2 clipping intensities (plants clipped or not clipped) for a total of 24 combinations replicated 6 times over a period of 90 days. We found a reduction in dry biomass as salinity increased, and this effect was greatest for P. pratensis. (1.94 g (SE 0.13) at 0 g/L NaCl to 0.22 g (SE 0.11) at 15 g/L NaCl). The R:S ratio of P. pratensis was reduced by salinity, but not for P. nuttalliana. Competitive importance of both species was reduced by clipping and by salinity, but the effect was greater and more consistent for P. pratensis. We conclude that salt concentration reduces plant growth and the effect of competition. [ABSTRACT FROM AUTHOR]

The effect of auxin treatment on the formation of apomictic -- endospermless grains in Poa pratensis was investigated in the study. Four polish cultivars: Skiz, Eska 46, Alicja, Ani as well as six breeding lines: POB 13, SK-W-33, SKW-15, SKW-35A, SK-W-35A and SK-W-35B were tested by embryological methods to reveal the reproduction pathway in greenhouse and field conditions. The obtained results showed that four genotypes, i.e. Alicja, Ani, Eska 46 and SK-W-35B are facultative apomictic and the other have formed seeds only on the apomictic way. In facultative apomicts it was found that in many cases both endospermless ovules containing embryo sacs with embryos or embryo sacs with well developed endosperm were formed. In the case of apomictic genotypes only endospermless seeds were observed. [ABSTRACT FROM AUTHOR]

Elemental sulfur (S) impregnated with Fe (S-Fe) fertilizer was evaluated on Kentucky bluegrass (KBG; Poa pratensis L.) in three glasshouse studies: (1) with/without Fe as S-Fe, ferrous sulfate (FS), or iron-ethylenediamine-N,N’-bis(2-hydroxyphenylacetic acid) (Fe-EDDHA) to soil (49 kg Fe ha−1), or foliar Fe-EDDHA (5 kg Fe ha−1) on two cultivars; (2) 0, 49, 197, or 592 kg Fe ha−1 of S-Fe deep mixed or surface mixed on two soils; (3) 0 or 49 kg Fe ha−1 applied as S-Fe on four cultivars. A field study comparing 0 and 49 kg Fe ha−1 applied as S-Fe to KBG was also conducted. Soil applied S-Fe was assimilated in shoots as efficiently as Fe-EDDHA applied to soil or foliage. Shoot Fe increased significantly with increasing S-Fe in a curvilinear response when deep mixed with soil and in a linear response when surface applied. However, no Fe source tested impacted yield or verdure in these studies. [ABSTRACT FROM PUBLISHER]

Kentucky bluegrass (Poa pratensis L.) is an important facultative apomictic temperate perennial grass species used for both forage and cultivated turf. Through apomixis, this species is able to propagate diverse and odd ploidy levels, resulting in many genetically distinct phenotypes. A wide range of diverse cultivars and accessions kentucky bluegrass have been previously characterized based on pedigree, common turf performance, and morphological characteristics to create a kentucky bluegrass cultivar classification system. The objectives of the current study were to assess the amount genetic divergence among kentucky bluegrass cultivars, experimental selections, and plant collections and revise/update the original pedigree, turf performance, and morphological characteristics kentucky bluegrass classification system using recently described kentucky bluegrass microsatellite [simple sequence repeat (SSR)] markers. In this study, 247 kentucky bluegrass cultivars, experimental selections, and collections were genotyped using 25 SSR markers. SSR markers showed a strong correlation between genetic relatedness as assessed by molecular markers and the original kentucky bluegrass classification system and also provided justification for a revision/update of the classification system. Traditional classification types that were supported by the current SSR analysis include BVMG, Compact, Compact-America, Julia, Mid-Atlantic, Midnight, and Shamrock types. Newly proposed classification types included Cynthia, Jefferson/Washington, Limousine, P-105, Sydsport, and three Eurasian types. The majority of cultivars, experimental selections, and collections were uniquely identified with the current set SSR markers. Genetic relationships of individuals as assessed by SSR markers closely matched known pedigrees. The current set of SSR markers can be used to rapidly genotype and assign new cultivars/accessions to kentucky bluegrass classification types and assess genetic relatedness among individuals and should be considered for use a kentucky bluegrass plant variety protection program. [ABSTRACT FROM AUTHOR]

Trinexapac-ethyl (TE) is a popular plant growth regulator in the turfgrass industry that inhibits gibberellic acid (GA) biosynthesis and effectively reduces leaf elongation and subsequent clipping production. This greenhouse sand culture experiment was conducted to determine effects of TE application on kentucky bluegrass (Poa pratensis L.) responses to salinity stress. The five salinity levels (0, 20, 40, 60, and 80 mM NaCl) were applied in nutrient solutions and TE treatments (0, 1, and 1.7 g/100 m²) were applied twice at 4-week intervals. Under non-saline conditions and low level salinity conditions, application of TE at 1 g/100 m² (TE1) increased turf quality (TQ), leaf total non-structural carbohydrates (TNC), and chlorophyll (Chl) content. In high salinity, TE1 alleviated the decline in TQ, antioxidant enzyme activities, leaf TNC, Chl, and K+ content. In addition, treated turf with TE at 1 g/100 m² had lower proline, Na+, and malondialdehyde (MDA) contents. However, the adverse effects of high salinities were more pronounced when turf was treated by TE at 1.7 g/100 m² (TE1.7), suggesting that effects of TE on salt tolerance vary with its dosages and salinity levels. We concluded that moderate inhibition of GA biosynthesis by TE enhances salt tolerance in kentucky bluegrass and suggest that enhancement is the result of the maintenance of antioxidant activities, leading to more root growth and greater levels of TNC and Chl content. Chemical names used: 4-(cyclopropyl-β-hydroxymethylene)-3, 5-dioxocyclohexanecarboxylic acid ethyl ester (trinexapac-ethyl). [ABSTRACT FROM AUTHOR]

Abstract: The first reports from and regarding the level of Neotyphodium colonization of grasses in Poland identified ratios ranging from 20% to 60%. To date, the studies of endophytes in Poland have focused mainly on the rate of endophyte infection in subsequent years of plantation use, the effect of endophytes on turf performance and the occurrence of endophytes in cultivars of meadow fescue, tall fescue and perennial ryegrass. The aforementioned studies were mainly conducted on breeding varieties of grasses. From 2007 to 2009, intensive studies on Neotyphodium occurrence in ecotypes of wild grasses in Poland were conducted. The results suggest that Neotyphodium species are quite common in Poland and colonize roughly 70% of semi-natural, used or abandoned grasslands. In this paper we review studies that have been conducted thus far in Poland regarding the occurrence of Neotyphodium spp. in grasses. [Copyright &y& Elsevier]

Six field trials were conducted in 2009 and 2010 to study postemergence control of annual bluegrass (Poa annua L. var. Hausskn Timm) in kentucky bluegrass (Poa pratensis L.) with mesotrione. Mesotrione was applied at 11 different rate and application intervals to an area of kentucky bluegrass that was naturally infested with annual bluegrass. Mesotrione rates of 56 g·ha-1 applied two or three times per week for a total of 10 applications or 84 g·ha-1 applied two times per week for a total of seven applications provided consistent control of annual bluegrass but required significant application labor and resulted in minor kentucky bluegrass injury. Other treatments that required fewer applications, 110 g·ha-1 applied twice per week for five applications or 186 g-ha-1 per week for three applications, also achieved high levels of control under high air temperatures, but control levels can vary significantly as temperatures fluctuate and seasons change. Mesotrione can successfully control annual bluegrass in kentucky bluegrass when frequent applications at low rates are applied or when environmental conditions are conducive to control. [ABSTRACT FROM AUTHOR]

Abstract: Poa pratensis, a type species for the grass family (Poaceae), is an important cool season grass that accumulates fructans as a polysaccharide reserve. We studied fructan contents and expression of candidate fructan metabolism genes during cold acclimation in three varieties of P. pratensis adapted to different environments: Northern Norway, Denmark, and the Netherlands. Fructan content increased significantly during cold acclimation and varieties showed significant differences in the level of fructan accumulation. cDNA sequences of putative fructosyltransferase (FT), fructan exohydrolase (FEH), and cold acclimation protein (CAP) genes were identified and cloned. In agreement with a function in fructan biosynthesis, transcription of a putative sucrose:fructan 6-fructosyltransferase (Pp6-SFT) gene was induced during cold acclimation and fructan accumulation in all three P. pratensis varieties. Transcription of putative PpFEH and PpCAP genes was also induced by cold acclimation; however, transcription of these two genes was several-fold higher in the variety from Norway compared to the other two varieties. The results presented here suggest that Pp6-SFT is involved in fructan biosynthesis in P. pratensis. FEHs have previously been suggested to be involved in fructan biosynthesis and freezing tolerance, and induced expression of PpFEH during fructan accumulation could also suggest a role in fructan biosynthesis. However, based on the different PpFEH transcription rates among varieties and similar expression of PpFEH and PpCAP, we suggest that PpFEH is more likely to be involved in mediating freezing tolerance, e.g., by regulating the cell osmotic potential through fructan degradation. [ABSTRACT FROM AUTHOR]

Kentucky bluegrass (Poa pratensis L.) is an important facultative apomictic temperate perennial grass species used for both forage and cultivated turf. Through apomixis, this species is able to propagate diverse and odd ploidy levels, resulting in many genetically distinct phenotypes. A wide range of diverse cultivars and accessions of kentucky bluegrass have been previously characterized based on common turf performance or morphological characteristics as well as by random amplified polymorphic DNA (RAPD) markers. Although previous characterization efforts have provided valuable information, the use of both morphological characteristics and RAPD markers for genetic diversity analysis has limitations. In the current report, we developed and characterized 88 novel microsatellite markers for kentucky bluegrass. Polymorphism for each marker was assessed in 265 kentucky bluegrass cultivars, experimental selections, collections, and hybrids. The number of alleles for individual microsatellites ranged from four to 81 with an average of 38.3 alleles per simple sequence repeat. These polymorphic microsatellite markers would be useful tools for investigating genetic diversity, creation of genetic linkage maps, assessment of levels of apomixis in cultivars and experimental varieties, and identification of aberrant progeny in apomictic kentucky bluegrass breeding programs. [ABSTRACT FROM AUTHOR]

Abstract: Decline of leafy spurge (Euphorbia esula) in the northern Great Plains of the US is generally viewed as a success story for biological control, but quality of the vegetation that survived the infestation is key to recovery of ecosystem function. In addition, effects of other invasive species, notably cool-season exotic grasses, must be taken into account. Objectives of this study were (1) to evaluate direction and significance of changes in biomass of native and exotic grasses, forbs, and leafy spurge and in plant species composition following control of leafy spurge by flea beetles and (2) to evaluate the relative effects of leafy spurge and exotic grasses on biomass of native grasses, biomass of forbs, and richness of native species. We monitored species composition (1998–2003 and 2008) and biomass (2000, 2002, 2003 and 2008) of these groups on spurge-infested and noninfested permanent plots at three sites with unbroken prairie sod in North Dakota, USA. We found little evidence, in terms of species richness or biomass of native grasses or forbs, that leafy spurge was being replaced by desirable native species, although desirable as well as weedy and exotic species were characteristic of 2008 vegetation at all three sites. Structural equation models revealed that leafy spurge had temporally intermittent negative effects on forb biomass and species richness, but no effects on native grasses. In contrast, exotic grass had consistently strong, negative effects on native grass biomass, as well as stronger negative effects than leafy spurge on native species richness. Although substantial native plant diversity remains at these sites, exotic grasses pose an important threat to these crucial building blocks of native prairie ecosystems. [Copyright &y& Elsevier]

The objective of this study was to determine the importance of protein synthesis during germination of white clover ( Trifolium repens) and Kentucky bluegrass ( Poa pratensis) and to assess the impact of osmopriming on seed germination in the presence of cadmium. Cycloheximide (Cx, 20 and 100 μM), a translation inhibitor, delayed germination and reduced final germination percentage in both species, although to a higher extent in P. pratensis than in T. repens. This suggests that in white clover, some proteins involved in reserve mobilisation and metabolic activation were already present in mature seeds. Actinomycin-D (Act-D; 20 and 100 μM) and α-amanitin (α-Am 20 μM), both inhibitors of transcription, did not reduce P. pratensis germination, demonstrating that for this species, transcription is not essential for radicle protrusion and that long-lived mRNA are used for initial protein synthesis. In contrast, incised white clover seeds showed delayed germination in presence of α-Am, suggesting that expression of some specific genes may help in the first germination steps. Osmopriming (PEG 8,000 60%) improved germination of seeds exposed to 100 μM Cd but the positive effect of this pretreatment was suppressed ( T. repens) or drastically mitigated ( P. pratensis) when Act-D or Cx were added to the priming medium. Cadmium reduced α- and β-amylase activities in germinating seeds but osmoprimed seeds exhibited higher activities than unprimed seeds. The presence of inhibitors in the priming medium reduced α- and β-amylase activities. These results suggest that the two studied species differed significantly in the mechanisms occurring during early seed germination and that the beneficial effect of priming requires gene expression and protein synthesis. [ABSTRACT FROM AUTHOR]

Persistency of grass associations is one of the way to counteract degradation of grasslands. The objective of the investigations carried out in 9-11 years of pasture use was to evaluate Poa pratensis persistence in sward. In the years 2005-07 Poa pratensis made 22.3-47.6% in the first regrowth of grass-clover sward and 39.3-52.1% of grass sward. The last two years were marked by a clear decrease of the studied species amount in the pasture association, irrespective of sward type and N fertilization dose. However, it was noted that the further increase of Poa trivialis and Deschampsia caespitosa share in spring sward, while Trifolium repens and Dactylis glomerata in summer. The higher competitiveness of Dactylis glomerata under conditions less suitable for Kentucky bluegrass development induced pasture productivity stabilization. Yet a higher abundance of this species in summer, like the growing Poa trivialis contribution in spring sward regrowth, has not ensured stabilization of pasture association in a marshy habitat. [ABSTRACT FROM AUTHOR]

Creeping bentgrass (Agrostis stolonifera L.; CBG) is a common weed in home lawns and golf course roughs in many regions of the United States. Currently, no herbicides are registered for selective control of CBG in cool-season grasses. The objective of this field study was to evaluate the ability of mesotrione and triclopyr ester to selectively remove CBG from Kentucky bluegrass (Poa pratensis L.) and tall fescue (Festuca arundinacea Schreb.). Mesotrione (0.14 and 0.21 kg⋅ha-1 a.i.) and triclopyr ester (0.56 and 1.12 kg⋅ha-1 a.i.) were applied on a 2-week interval two, three, or four times in Connecticut and Maryland in 2005, and three or four times in Maryland in 2006. Two applications of mesotrione at 0.21 kg⋅ha-1 a.i. provided marginally acceptable CBG control, but three or four applications at 0.14 or 0.21 kg⋅ha-1 a.i. provided excellent CBG control. Mesotrione elicited little or no injury to Kentucky bluegrass, but generally caused objectionable injury in tall fescue for about 7 to 14 d after each application. Triclopyr applied at 0.56 kg⋅ha-1 a.i. reduced CBG cover, but the level of control generally was unacceptable, regardless of application frequency. Three or four applications of triclopyr (1.12 kg⋅ha-1 a.i.) effectively controlled CBG in Connecticut in 2005 and Maryland in 2006. Triclopyr caused no visual injury to tall fescue, regardless of rate or application frequency. Four triclopyr applications to Kentucky bluegrass, however, were phytotoxic and reduced stand density, especially at the high rate (1.12 kg⋅ha-1 a.i.). Three summer applications of mesotrione (0.14 kg⋅ha-1 a.i.) or triclopyr (1.12 kg⋅ha-1 a.i.) provided the best combination of turfgrass safety and CBG control. Chemical names used: [(3,5,6-trichloro-2-pyridinyl)oxy]acetic acid (triclopyr ester); 2-[4-(methylsulfonyl)-2-nitrobenzoyl]-l,3-cycilohexanedione (mesotrione) [ABSTRACT FROM AUTHOR]

Waterlogging (WL) affects the growth and physiological responses of turfgrass. The objectives of this study were to compare the relative WL tolerance of Kentucky bluegrass (Poa pratensis L.) cultivars and to investigate the physiological responses of shoots and roots to WL. Ten cultivars differing in growth habit were subjected to 30 d of WL. The turf quality (TQ) and soil redox potential (Eh), as well as the chlorophyll concentration (Chl), decreased with increasing periods of WL. Among all cultivars, root dry weight (RDW) decreased 16.7% to 39.9% under 10 d and 30.0% to 60% under 30 d of WL, respectively. Waterlogging increased the root electrolyte leakage (REL) from 0.6% to 53.2% under 10 d and from 29.1% to 98.0% under 30 d of WL for all cultivars, respectively. The best correlations were observed between Eh and TQ (r = 0.74), REL and TQ (r = 0.75), RDW and root water-soluble carbohydrate content (RWSC) (r = 0.74), and root oxidase activity and RWSC (r = 0.63), respectively. 'Moonlight', 'Serene', and 'Champagne' showed better tolerance to short-term WL conditions, whereas 'Kenblue' and 'Eagleton' were the least tolerant cultivars. 'Limousine', 'Unique', 'Awesome', 'Julia', and "Midnight II' ranked in the middle group. Variations in WL tolerance among Kentucky bluegrasses could potentially be used for enhancing turfgrass management. [ABSTRACT FROM AUTHOR]

'Dura Blue' and 'Thermal Blue' hybrid bluegrass have been selected for heat and drought tolerance. These grasses offer alternatives to traditional Kentucky bluegrass and tall fescue in the transition zone. Experiments were conducted in two locations during 2003 and 2004 at the University of Tennessee in Knoxville, Tenn. Nitrogen (N) was applied at 50, 150, or 300 kg·ha-1 N per year to 'Apollo' Kentucky bluegrass, 'Dura Blue', and 'Thermal Blue' hybrid bluegrass, and 'Dynasty' and 'Kentucky 31' tall fescue. The main effects of turfgrass and N were significant for color and quality observations. However, their interactions were not significant; therefore, only the main effects are shown. Acceptable turfgrass color (>6) and quality (>6) was observed for all varieties in May, August, and November. All N regimens showed acceptable turfgrass color and quality. However, 150 kg·ha-1 N per year was required to achieve optimum color and quality. 'Kentucky 31' produced higher clipping dry weights when N was applied at 50 kg·ha-1 per year than the other varieties. Nitrogen applied at 150 and 300 kg·ha-1 per year on 'Kentucky 31' and 'Thermal Blue' produced higher clipping dry weights than the other varieties. 'Dynasty' and 'Kentucky 31' had similar brown patch incidences at each nitrogen level. Increases in brown patch incidence occurred as N levels decreased from 300 (21%) to 50 kg·ha-1 per year (31%) for 'Dynasty' and 'Kentucky 31'. Dollar spot incidence occurred on all bluegrass varieties from 7% to 24%. However, dollar spot decreased with increased N fertility. All turfgrass species tested were acceptable for use in the transition zone. [ABSTRACT FROM AUTHOR]

Negative impacts of invasive plants on natives have been well documented, but much less is known about whether invasive plants can cause population level declines. We used demographic models to investigate the effects of two invasive grasses on the demography and population growth of Anemone patens, a long-lived native perennial of North American grasslands. Demographic data of A. patens growing in patches characterized by Bromus inermis, Poa pratensis, or native grasses were used to parameterize integral projection models. Models based on both average conditions and those allowing for environmental stochasticity indicate that A. patens is slowly increasing in patches of native grass (λ = 1.02) and declining in patches of invasive grasses, particularly those dominated, by B. inermis (λ = 0.93). Extinction probabilities indicate that A. patens should persist in native grass patches, but has a much higher probability of extinction in Bromus patches compared to Poa patches. While sensitivity analyses showed that survival had the biggest effect on population growth rates in all habitats, results of a Life Table Response Experiment (LTRE) revealed that slower individual growth rates in patches of invasive grasses contributed the most to the observed reduction in population growth. These results suggest that invasive grasses may cause slow declines in A. patens, despite short-term coexistence, and that controlling B. inermis only would not be sufficient to ensure A. patens persistence. [ABSTRACT FROM AUTHOR]

Expansion of turf area together with irrigation have caused concern because of the increased demand for the scarce water resources in arid and semi-arid regions. Kentucky bluegrass (KBG) (Poa pratensis) has been widely used to establish turf, and selecting varieties with good drought resistance is important in reducing irrigation requirements. Its seedling stage is the key step to successful establishment. Therefore, pot experiments in the greenhouse were carried out to assess drought resistance in 11 KBG varieties. The rate of germination, survival rate, and vertical growth rate of each variety increased as field water capacity (FWC) decreased from 70% (FWC 70) to 50% (FWC 50), and then decreased from 50% to 30% (FWC 30). Tillering rate and shoot-to-root ratio decreased as FWC decreased from FWC 70 to FWC 30. Proline content and electrolyte leakage (EL) in leaves of KBG at FWC 50 was lower than those at FWC 30 and FWC 70. Uunder FWC 30, representing drought stress, rate of germination, survival rate of seedlings, vertical growth rate, and shoot-to-root ratio were significantly different among the 11 varieties. Based on the rate of germination, 'Barlin' and 'Baron' performed best. According to the survival rate of seedlings, 'Baron' performed best. For vertical growth rate, 'Barlin' and 'Compact' were superior to others. 'Barlin' was the best variety for lower shoot-to-root ratio. 'Barlin' and 'Baron' had higher proline accumulation and greater cell membrane stability (lower EL) in leaves. Using rate of germination, survival rate, vertical growth rate, shoot-to-root ratio, proline content, and EL as analysis factors, cluster analysis indicated that 'Baron' and 'Barlin' had better resistance to drought than other varieties, and suggested that they are suitable for sowing in arid and semi-arid regions because of their strong drought resistance at the seedling stage. [ABSTRACT FROM AUTHOR]

Abstract: Interspecific plant competition has been hypothesized to alter effects of early-season ozone (O3) stress. A phytometer-based approach was utilized to investigate O3 effects on growth and nutritive quality of Poa pratensis grown in monoculture and in mixed cultures with four competitor-plant species (Anthoxanthum odoratum, Achillea millefolium, Rumex acetosa and Veronica chamaedrys). Mesocosms were exposed during April/May 2000–2002 to charcoal-filtered air+25ppb O3 (control) or non-filtered air+50ppb O3 (elevated O3). Biomass production was not affected by O3, but foliar injury symptoms were observed in May 2002. Early-season O3 exposure decreased relative food value of P. pratensis by an average of 8%, which is sufficient to have nutritional implications for its utilization by herbivores. However, forage quality response to O3 was not changed by interspecific competition. Lack of injury and nutritive quality response in P. pratensis harvested in September may reflect recovery from early-season O3 exposure. [Copyright &y& Elsevier]

Question: Does the spatial pattern of nutrient supply modify community biomass responses to changes in both species composition and richness? Location: Duke University Phytotron (Durham, North Carolina, USA). Methods: We conducted a microcosm experiment to evaluate individual plant and whole community responses to species richness, species composition and soil nutrient heterogeneity. The experiment consisted of seven levels of species composition (all possible combinations of Lolium perenne, Poa pratensis and Plantago lanceolata) crossed with three levels of soil nutrient distribution (homogeneous, heterogeneous-up, and heterogeneous-down, where up and down indicates the location of a nutrient patch in either the upper or the lower half of the soil column, respectively). Results: Communities containing Plantago and Lolium responded to nutrient heterogeneity by increasing above- and below-ground biomass. Nutrient heterogeneity also increased size inequalities among individuals of these species. Significant species composition × nutrient heterogeneity interactions on community biomass and individual size inequality were observed when nutrient patches were located in the upper 10 cm of the soil columns. However, root proliferation in nutrient patches was equivalent regardless of the vertical placement of the patch. Conclusions: Our results suggest that nutrient heterogeneity may interact with plant species composition to determine community biomass, and that small-scale vertical differences in the location of nutrient patches affect individual and community responses to this heterogeneity. [ABSTRACT FROM AUTHOR]

• Background and Aims To improve the management of grass communities, early plant development was compared in three species with contrasting growth forms, a caespitose (Lolium perenne), a rhizomatous (Poa pratensis) and a caespitose–stoloniferous species (Agrostis stolonifera).• Methods Isolated seedlings were grown in a glasshouse without trophic constraints for 37 d (761 °Cd). The appearance of leaves and their location on tillers were recorded. Leaf appearance rate (LAR) on the tillers and site-filling were calculated. Tillering was modelled based on the assumption that tiller number increases with the number of leaves produced on the seedling main stem. Above- and below-ground parts were harvested to compare biomass.• Key Results Lolium perenne and A. stolonifera expressed similar bunch-type developments. However, root biomass was approx. 30 % lower in A. stolonifera than in L. perenne. Poa pratensis was rhizomatous. Nevertheless, the ratio of above-ground : below-ground biomass of P. pratensis was similar to that of L. perenne. LAR was approximately equal to 0·30 leaf d−1 in L. perenne, and on the main stem and first primary tillers of A. stolonifera. LAR on the other tillers of A. stolonifera was 30 % higher than on L. perenne. For P. pratensis, LAR was 30 % lower than on L. perenne, but the interval between the appearance of two successive shoots from rhizomes was 30 % higher than the interval between two successive leaf stages on the main stem. Above-ground parts of P. pratensis first grew slower than in the other species to the benefit of the rhizomes, whose development enhanced tiller production.• Conclusions Lolium perenne had the fastest tiller production at the earliest stages of seedling development. Agrostis stolonifera and P. pratensis compensated almost completely for the delay due to higher LAR on tillers or ramets compared with L. perenne. This study provides a basis for modelling plant development. [ABSTRACT FROM AUTHOR]

Late fall N fertilization of cool-season turfgrass in northern climates is a common practice. Previous research has been focused in climates where freezing temperatures prevail. Research in more moderate northern climates where turf may not go through winter dormancy is scarce. Four fertilizer N sources and an untreated control were applied in four different months (November, December, January, or February) to perennial ryegrass (Lolium perenne L.) in Puyallup, Wash., and to kentucky bluegrass (Poa pratensis L.) In Pullman, Wash., to compare their effects in moderate (Puyallup) and freezing (Pullman) winter climates. In Pullman, only November applications of ammonium sulfate (AmS) or polymer coated sulfur coated urea (PCSCU) enhanced winter turfgrass quality. In Puyallup, November or December application of AmS, PCSCU, or polymer coated urea (PCU) resulted in enhanced winter quality. Polymer coated urea yielded a delayed initial response and a longer residual effect in the spring. Isobutylidenediurea (IBDU) did not improve winter turf quality in either Pullman or Puyallup. Although there was no quality response following January fertilizer application, there was suppression of red thread [Laetisaria fuciformis (McAlpine) Burds.] symptoms in Puyallup, indicating N uptake. Late fall fertilizer N in eastern Washington should be confined to November, using soluble or more quickly available slow-release nitrogen fertilizers. The application window can be extended to December in western Washington, and more slowly available coated ureas can be effectively used. [ABSTRACT FROM AUTHOR]

Presents abstracts of several studies on forages and pastures. "Nutritive Quality of Poa Pratensis in Model Grassland Communities Exposed to Ground-Level Ozone," by D. Dodson, J,. Bender, J. Lin and R. Muntifering; "Effect of Increased Cutting Height of Corn Silage on Nutritive Value, Milk Yield and Milk Composition," by D. Domiguez-Diaz and L. D. Satter; "Forage Production and Quality of Triticale Cultivars in the Tennessee Valley," by M. Lema and E. Cebert; "Kura Clover Spreading Ability With Grass Competition," by B. Kim and K. Albrecht....abstracts, patures, forage plants, kentucky bluegrass, grasslands, corn-silage, milk production, milk-composition, triticale.