1. PDGF-BB inhibits intervertebral disc cell apoptosis in vitro.
- Author
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Presciutti, Steven M., Paglia, David N., Karukonda, Teja, Soung, Do Yu, Guzzo, Rosa, Drissi, Hicham, and Moss, Isaac L.
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PLATELET-derived growth factor ,INTERVERTEBRAL disk ,APOPTOSIS ,BACKACHE ,CELL proliferation ,GENE expression ,IMMUNOHISTOCHEMISTRY ,FLOW cytometry - Abstract
ABSTRACT Degeneration of the intervertebral disc (IVD) results in deterioration of the spinal motion segment and can lead to debilitating back pain. Given the established mitotic and anti-apoptotic effects of recombinant human platelet-derived growth factor-BB (rhPDGF-BB) in a variety of cell types we postulated that rhPDGF-BB might delay disc cell degeneration through inhibition of apoptosis. To address this hypothesis, we treated human IVD cells isolated from five independent patients with rhPDGF-BB in monolayer and 3D pellet cultures. The anti-apoptotic potential, cell proliferative capacity, morphology/pellet differentiation, and gene expression of PDGF-treated IVD cells were evaluated via flow cytometry/immunohistochemistry, MTT assays, histology, and quantitative RT-PCR, respectively. We found that rhPDGF-BB treatment significantly inhibited cell apoptosis, increased cell proliferation and matrix production, and maintained mRNA expression of critical extracellular matrix genes. This study suggests two possible mechanisms for the anti-degenerative effects of rhPDGF-BB on human IVD cells. First, PDGF treatment strongly inhibited IVD cell apoptosis in 3D cultures. Second, rhPDGF-BB acts as an anabolic agent, promoting maintenance of IVD cell phenotype in 3D culture, based on the molecular and protein expression analysis. We speculate that rhPDGF-BB may be used as a biologic treatment to target early degenerative IVD disease in the future. © 2014 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 32:1181-1188, 2014. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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