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1. Dutch patients, retail chicken meat and poultry share the same ESBL genes, plasmids and strains

Author

Leverstein-van Hall, M.A., Dierikx, C.M., Cohen Stuart, J., Voets, G.M., Munckhof, M.P. van den, Essen-Zandbergen, A. van, Platteel, T., Fluit, A.C., Sande-Bruinsma, N. van de, Scharinga, J., Bonten, M.J., Mevius, D.J., Sturm, P.D., Advances in Veterinary Medicine, and Dep Infectieziekten Immunologie

Subjects
Veterinary medicine, spectrum beta-lactamases, Antibiotic resistance, Epidemiology, medicine.disease_cause, multi-locus sequence typing, Polymerase Chain Reaction, Poultry, law.invention, Plasmid, law, Zoonoses, polycyclic compounds, Cluster Analysis, infections, humans, Escherichia coli Infections, Polymerase chain reaction, Netherlands, Antibacterial agent, Molecular Epidemiology, food and beverages, General Medicine, Bacterial Typing Techniques, Infectious Diseases, salmonella-enterica, Carrier State, Plasmids, trends, Microbiology (medical), Meat, Genotype, Bioinformatica & Diermodellen, prevalence, Biology, beta-Lactamases, diversity, Microbiology, plasmid, Bio-informatics & Animal models, Escherichia coli, medicine, Animals, Humans, emergence, Epidemiology, Bio-informatics & Animal models, human, Typing, Genotyping, Epidemiologie, resistant escherichia-coli, Molecular epidemiology, Pathogenesis and modulation of inflammation Infection and autoimmunity [N4i 1], zoonosis, biochemical phenomena, metabolism, and nutrition, bacterial infections and mycoses, Molecular Typing, extended-spectrum, Epidemiologie, Bioinformatica & Diermodellen, Multilocus sequence typing, bacteria, Multilocus Sequence Typing
Abstract

Item does not contain fulltext Intestinal carriage of extended-spectrum beta-lactamase (ESBL) -producing bacteria in food-producing animals and contamination of retail meat may contribute to increased incidences of infections with ESBL-producing bacteria in humans. Therefore, distribution of ESBL genes, plasmids and strain genotypes in Escherichia coli obtained from poultry and retail chicken meat in the Netherlands was determined and defined as 'poultry-associated' (PA). Subsequently, the proportion of E. coli isolates with PA ESBL genes, plasmids and strains was quantified in a representative sample of clinical isolates. The E. coli were derived from 98 retail chicken meat samples, a prevalence survey among poultry, and 516 human clinical samples from 31 laboratories collected during a 3-month period in 2009. Isolates were analysed using an ESBL-specific microarray, sequencing of ESBL genes, PCR-based replicon typing of plasmids, plasmid multi-locus sequence typing (pMLST) and strain genotyping (MLST). Six ESBL genes were defined as PA (bla(CTX-M-1) , bla(CTX-M-2) , bla(SHV-2) , bla(SHV-12) , bla(TEM-20) , bla(TEM-52) ): 35% of the human isolates contained PA ESBL genes and 19% contained PA ESBL genes located on IncI1 plasmids that were genetically indistinguishable from those obtained from poultry (meat). Of these ESBL genes, 86% were bla(CTX-M-1) and bla(TEM-52) genes, which were also the predominant genes in poultry (78%) and retail chicken meat (75%). Of the retail meat samples, 94% contained ESBL-producing isolates of which 39% belonged to E. coli genotypes also present in human samples. These findings are suggestive for transmission of ESBL genes, plasmids and E. coli isolates from poultry to humans, most likely through the food chain. 01 juni 2011

Published
2011

2. Differences in the antibiotic susceptibility of human Escherichia coli with poultry-associated and non-poultry-associated extended-spectrum beta-lactamases.

Author

Platteel, T., Leverstein-Van Hall, M., Cohen Stuart, J., Voets, G., Munckhof, M., Scharringa, J., Sande, N., Fluit, A., and Bonten, M.

Subjects
*ANTIBIOTICS, *ESCHERICHIA coli, *BETA lactamases, *MICROBIAL sensitivity tests, *PLASMIDS, *POULTRY, *FOODBORNE diseases
Abstract

The concurrent presence of bla and bla genes on similar plasmids of Escherichia coli isolated from poultry, chicken meat and humans supports the occurrence of food-borne transmission of extended-spectrum beta-lactamase (ESBL) genes. ESBL-producing E. coli (ESBL- E. coli) are most frequently detected in hospitalised patients and are known to spread in healthcare settings. We hypothesised that poultry-associated (PA) ESBL genes are predominant in the community, where acquisition is fuelled by food contamination, whereas non-PA ESBL genes are predominant in hospitals, with acquisition fuelled by cross-transmission. Then, differences in antimicrobial selective pressure in hospitals and poultry would create differences in co-resistance between PA and non-PA ESBL- E. coli. We, therefore, determined the prevalence and co-resistance of PA and non-PA ESBL- E. coli in community-acquired and nosocomial urinary tract infections in humans and bla and bla isolates from poultry. A total of 134 human ESBL- E. coli urine isolates were included in this study. Isolates containing bla or bla were considered to be PA, with the remainder being non-PA. Also, 72 poultry ESBL- E. coli were included. Minimum inhibitory concentration (MIC) values were determined by broth microdilution. The prevalence of PA ESBL genes in isolates obtained in general practice and hospitals was 28 % versus 30 % (n.s.). Human PA ESBL- E. coli were more frequently susceptible to ciprofloxacin (51 % vs. 25 %; p = 0.0056), gentamicin (86 % vs. 63 %; p = .0.0082), tobramycin (91 % vs. 34 %; p = 0.0001) and amikacin (98 % vs. 67 %; p = 0.0001) compared to human non-PA ESBL- E. coli. PA ESBL- E. coli are not more prevalent in community acquired than nosocomial urine samples, but are more often susceptible to ciprofloxacin and aminoglycosides than non-PA ESBL- E. coli. This does not support the existence of different reservoirs of ESBL genes. [ABSTRACT FROM AUTHOR]

Published
2013
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