1. A cryptic plasmid from Nocardia orientalis NRRL 2452, a vancomycin producer
- Author
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Louis R. Fare, Yong K. Oh, Louis J. Nisbet, Jenifer B. Widger, and Dean P. Taylor
- Subjects
Pharmacology ,biology ,Streptomycetaceae ,Chromosome Mapping ,Drug Resistance, Microbial ,Nocardia ,biology.organism_classification ,Molecular biology ,Microbiology ,Restriction enzyme ,chemistry.chemical_compound ,Plasmid ,chemistry ,Vancomycin ,Drug Discovery ,medicine ,Actinomycetales ,Bacteria ,DNA ,Plasmids ,medicine.drug - Abstract
A plasmid was found in Nocardia orientalis (formerly Streptonryces orientalis). Physical characterization of the plasmid DNA indicates a size of 33.5 kb and a single cleavage site for EcoR I. The presence of plasmid, and variation in its copy member, did not directly affect vancomycin resistance or production levels. The plasmid represents the first to be isolated and characterized from a glycopeptide-producing nocardia.
- Published
- 1986