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49. Reinventing Potato as a Diploid Inbred Line-Based Crop.

Author

Jansky, Shelley H., Charkowski, Amy O., Douches, David S., Gusmini, Gabe, Richael, Craig, Bethke, Paul C., Spooner, David M., Novy, Richard G., De Jong, Hielke, De Jong, Walter S., Bamberg, John B., Thompson, A. L., Bizimungu, Benoit, Holm, David G., Brown, Chuck R., Haynes, Kathleen G., Sathuvalli, Vidyasagar R., Veilleux, Richard E., Miller Jr., J. Creighton, and Bradeen, Jim M.

Subjects
POTATO breeders, DIPLOIDY, POLYPLOIDY, HETEROZYGOSITY, TETRAPLOIDY, PLANTS, PLANT chromosomes
Abstract

The third most important food crop worldwide, potato (Solanum tuberosum L.) is a tetraploid outcrossing species propagated from tubers. Breeders have long been challenged by polyploidy, heterozygosity, and asexual reproduction. It has been assumed that tetraploidy is essential for high yield, that the creation of inbred potato is not feasible, and that propagation by seed tubers is ideal. In this paper, we question those assumptions and propose to convert potato into a diploid inbred line-based crop propagated by true seed. Although a conversion of this magnitude is unprecedented, the possible genetic gains from a breeding system based on inbred lines and the seed production benefits from a sexual propagation system are too large to ignore. We call on leaders of public and private organizations to come together to explore the feasibility of this radical and exciting new strategy in potato breeding. [ABSTRACT FROM AUTHOR]

Published
2016
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50. Linking the potato genome to the conserved ortholog set (COS) markers.

Author

Lindqvist-Kreuze, Hannele, Kwangsoo Cho, Portal, Leticia, Rodríguez, Flor, Simon, Reinhard, Mueller, Lukas A., Spooner, David M., and Bonierbale, Merideth

Subjects
LINKAGE (Genetics), PLANT genetics, POTATO genetics, BIOMARKERS, PLANTS, NATURAL selection, PLANT phylogeny
Abstract

Background: Conserved ortholog set (COS) markers are an important functional genomics resource that has greatly improved orthology detection in Asterid species. A comprehensive list of these markers is available at Sol Genomics Network (http://solgenomics.net/) and many of these have been placed on the genetic maps of a number of solanaceous species. Results: We amplified over 300 COS markers from eight potato accessions involving two diploid landraces of Solanum tuberosum Andigenum group (formerly classified as S. goniocalyx, S. phureja), and a dihaploid clone derived from a modern tetraploid cultivar of S. tuberosum and the wild species S. berthaultii, S. chomatophilum, and S. paucissectum. By BLASTn (Basic Local Alignment Search Tool of the NCBI, National Center for Biotechnology Information) algorithm we mapped the DNA sequences of these markers into the potato genome sequence. Additionally, we mapped a subset of these markers genetically in potato and present a comparison between the physical and genetic locations of these markers in potato and in comparison with the genetic location in tomato. We found that most of the COS markers are single-copy in the reference genome of potato and that the genetic location in tomato and physical location in potato sequence are mostly in agreement. However, we did find some COS markers that are present in multiple copies and those that map in unexpected locations. Sequence comparisons between species show that some of these markers may be paralogs. Conclusions: The sequence-based physical map becomes helpful in identification of markers for traits of interest thereby reducing the number of markers to be tested for applications like marker assisted selection, diversity, and phylogenetic studies. [ABSTRACT FROM AUTHOR]

Published
2013
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