1. [2-(14)C-Methylerythritol 2,4-cyclodiphosphate incorporation into bacterial and plant isoprenoids].
- Author
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Mazikin KV, Ershov IuV, goncharenko AV, and Ostrovskiĭ DN
- Subjects
- Carbon Isotopes metabolism, Carbon Isotopes pharmacology, Erythritol metabolism, Erythritol pharmacology, Sugar Phosphates pharmacology, Bacteria metabolism, Erythritol analogs & derivatives, Plants metabolism, Sugar Phosphates metabolism, Terpenes metabolism
- Abstract
To work out a test system for studying the inhibition of isoprenoid biosynthesis through non-mevalonate pathway, the conversion of 2-(14)C-methylerythritol 2,4-cyclodiphosphate (14C-MEC) into isoprenoids of three bacteria species and into plastids of 11 higher plant species was studied. The highest efficiency (up to 63%) of the process was observed with chromoplasts from narcissus (Narcissus pseudonarcissus L.) and celandine (Chelidonium majus L.). Twenty five percent of added 14C-MEC was recovered in an isoprenoid fraction of red pepper (Capsicum annuum L.) chromoplasts. Thus, these three models can be used to test antibiotic inhibitors of isoprenoid biosynthesis.
- Published
- 2009