11 results on '"Boqun Li"'
Search Results
2. Rhizospheric microbiomics integrated with plant transcriptomics provides insight into the Cd response mechanisms of the newly identified Cd accumulator Dahlia pinnata
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Xiong, Li, Boqun, Li, Tao, Jin, Huafang, Chen, Gaojuan, Zhao, Xiangshi, Qin, Yongping, Yang, and Jianchu, Xu
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Plant Science - Abstract
Phytoremediation that depends on excellent plant resources and effective enhancing measures is important for remediating heavy metal-contaminated soils. This study investigated the cadmium (Cd) tolerance and accumulation characteristics of Dahlia pinnata Cav. to evaluate its Cd phytoremediation potential. Testing in soils spiked with 5–45 mg kg–1 Cd showed that D. pinnata has a strong Cd tolerance capacity and appreciable shoot Cd bioconcentration factors (0.80–1.32) and translocation factors (0.81–1.59), indicating that D. pinnata can be defined as a Cd accumulator. In the rhizosphere, Cd stress (45 mg kg–1 Cd) did not change the soil physicochemical properties but influenced the bacterial community composition compared to control conditions. Notably, the increased abundance of the bacterial phylum Patescibacteria and the dominance of several Cd-tolerant plant growth–promoting rhizobacteria (e.g., Sphingomonas, Gemmatimonas, Bryobacter, Flavisolibacter, Nocardioides, and Bradyrhizobium) likely facilitated Cd tolerance and accumulation in D. pinnata. Comparative transcriptomic analysis showed that Cd significantly induced (P < 0.001) the expression of genes involved in lignin synthesis in D. pinnata roots and leaves, which are likely to fix Cd2+ to the cell wall and inhibit Cd entry into the cytoplasm. Moreover, Cd induced a sophisticated signal transduction network that initiated detoxification processes in roots as well as ethylene synthesis from methionine metabolism to regulate Cd responses in leaves. This study suggests that D. pinnata can be potentially used for phytoextraction and improves our understanding of Cd-response mechanisms in plants from rhizospheric and molecular perspectives.
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- 2022
- Full Text
- View/download PDF
3. Mutation of barley HvPDIL5-1 improves resistance to yellow mosaic virus disease without growth or yield penalties
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Chunyuan Cheng, Jinhong Kan, Shanshan Li, Congcong Jiang, Xiaoyan He, Huiquan Shen, Rugen Xu, Boqun Li, Zongyun Feng, and Ping Yang
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Plant Science - Abstract
The soil-borne yellow mosaic virus disease, which is caused by the bymoviruses barley yellow mosaic virus (BaYMV) and/or barley mild mosaic virus (BaMMV), seriously threatens winter barley production in Europe and East Asia. Both viruses are transmitted by the soil-borne plasmodiophorid Polymyxa graminis and are difficult to eliminate through chemical or physical measures in the field, making breeding for resistant cultivars the optimal strategy for disease control. The resistance locus rym1/11 was cloned encoding the host factor gene Protein Disulfide Isomerase Like 5-1 (PDIL5-1), whose loss-of-function variants confer broad-spectrum resistance to multiple strains of BaMMV/BaYMV. Most resistance-conferring variants have been identified in six-rowed barley landraces/historic cultivars, and their introgression into modern two-rowed malting cultivars is difficult because PDIL5-1 is located in a peri-centromeric region with suppressed recombination. In this study, we used CRISPR/Cas9 genome editing to modify PDIL5-1 in the BaYMV/BaMMV-susceptible elite malting barley cv. ‘Golden Promise’ and obtained the mutants pdil5-1-a and pdil5-1-b. PDIL5-1 in the pdil5-1-a mutant encodes a protein lacking a cysteine residue, and pdil5-1-b contains a protein-coding frameshift. Both mutants were completely resistant to BaYMV. The knockout mutant pdil5-1-b showed complete BaMMV resistance, while pdil5-1-a showed decreased viral accumulation but no disease symptoms if compared to ‘Golden Promise’. Both PDIL5-1 edited lines, as well as the previously produced EMS-induced pdil5-1 mutant ‘10253-1-5’ in the elite malting barley cv. ‘Barke’ background, displayed no growth or yield penalties in garden experiments or bymovirus-free field trials. Line ‘10253-1-5’ showed improved resistance and yield performance compared to the wild-type and its sibling line when grown in infectious fields. Therefore, genome editing of the host factor gene PDIL5-1 could facilitate the breeding of barley varieties with resistance to bymoviruses.
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- 2022
- Full Text
- View/download PDF
4. Cd accumulation characteristics of Salvia tiliifolia and changes of rhizospheric soil enzyme activities and bacterial communities under a Cd concentration gradient
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Xiong Li, Di Chen, Boqun Li, and Yongping Yang
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0106 biological sciences ,chemistry.chemical_classification ,Cadmium ,Rhizosphere ,biology ,Phosphorus ,Soil Science ,chemistry.chemical_element ,04 agricultural and veterinary sciences ,Plant Science ,biology.organism_classification ,01 natural sciences ,Salvia tiliifolia ,Phytoremediation ,chemistry ,Soil pH ,Botany ,Shoot ,040103 agronomy & agriculture ,0401 agriculture, forestry, and fisheries ,Organic matter ,010606 plant biology & botany - Abstract
This study aimed to explore the phytoremediation potential of Salvia tiliifolia for cadmium (Cd)-contaminated soils and to understand the interactions in soil-Cd-plant systems. We assessed the Cd tolerance and accumulation characteristics of S. tiliifolia grown in soils with 0, 5, 25, and 50 mg kg− 1 Cd for 3 months. We also analysed the changes in physicochemical properties, enzyme activities, and bacterial community composition in the rhizosphere soils of S. tiliifolia. S. tiliifolia could tolerate all Cd concentrations with unchanged biomass. Cd concentrations (21.50–189.50 mg kg− 1) in roots of S. tiliifolia and corresponding bioconcentration factors (3.79–4.30) were much higher than those in shoots, and Cd translocation factors (0.03–0.23) were thereby very low. Rhizosphere pH decreased with increasing concentrations of Cd. Soil pH and rich organic matter may jointly affect the soil available Cd. Ultimately, soil enzyme activities involved in carbon, nitrogen, and phosphorus cycles were affected to varying degrees, and bacterial diversity and richness were slightly influenced by Cd stress. Interestingly, bacterial ABC transporters were induced by Cd stress during this process. S. tiliifolia can be designed as a Cd excluder. The induced soil bacterial ABC transporters may contribute to Cd detoxification and accumulation in both soil microbial communities and S. tiliifolia plants.
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- 2021
5. Gamma-Aminobutyric Acid Enhances Cadmium Phytoextraction by Coreopsis grandiflora by Remodeling the Rhizospheric Environment
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Yingqi Huang, Boqun Li, Huafang Chen, Jingxian Li, Jianchu Xu, and Xiong Li
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gamma-aminobutyric acid ,heavy metal ,phytoextraction ,soil amendment ,plant growth-promoting rhizobacteria ,Ecology ,Plant Science ,Ecology, Evolution, Behavior and Systematics - Abstract
Gamma-aminobutyric acid (GABA) significantly affects plant responses to heavy metals in hydroponics or culture media, but its corresponding effects in plant–soil systems remain unknown. In this study, different GABA dosages (0–8 g kg−1) were added to the rhizosphere of Coreopsis grandiflora grown in Cd-contaminated soils. Cd accumulation in the shoots of C. grandiflora was enhanced by 38.9–159.5% by GABA in a dose-dependent approach because of accelerated Cd absorption and transport. The increase in exchangeable Cd transformed from Fe-Mn oxide and carbonate-bound Cd, which may be mainly driven by decreased soil pH rather than GABA itself, could be a determining factor responsible for this phenomenon. The N, P, and K availability was affected by multiple factors under GABA treatment, which may regulate Cd accommodation and accumulation in C. grandiflora. The rhizospheric environment dynamics remodeled the bacterial community composition, resulting in a decline in overall bacterial diversity and richness. However, several important plant growth-promoting rhizobacteria, especially Pseudomonas and Sphingomonas, were recruited under GABA treatment to assist Cd phytoextraction in C. grandiflora. This study reveals that GABA as a soil amendment remodels the rhizospheric environment (e.g., soil pH and rhizobacteria) to enhance Cd phytoextraction in plant–soil systems.
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- 2023
6. Effects of soil properties on accumulation characteristics of copper, manganese, zinc, and cadmium in Chinese turnip
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Di Chen, Yongping Yang, Boqun Li, and Xiong Li
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0106 biological sciences ,BCF, bioconcentration factor ,Soil composition ,chemistry.chemical_element ,Chromosomal translocation ,Bioconcentration ,Plant Science ,Manganese ,Zinc ,Ca, calcium ,010603 evolutionary biology ,01 natural sciences ,Article ,DW, dry weight ,Cd, cadmium ,TF, translocation factor ,Soil pH ,lcsh:Botany ,Mn, manganese ,Pb, lead ,Turnip ,EC, electrical conductivity ,lcsh:QH301-705.5 ,Cu, copper ,Ecology, Evolution, Behavior and Systematics ,HM, heavy metal ,Cadmium ,food and beverages ,Zn, zinc ,Phytoremediation ,lcsh:QK1-989 ,Heavy metal ,chemistry ,lcsh:Biology (General) ,Environmental chemistry ,Soil water ,CEC, cation exchange capacity ,010606 plant biology & botany - Abstract
Clarifying the mechanisms of heavy metal (HM) accumulation and translocation from soil-root-leaf is crucial to coping with soil HM pollution. In this study, we analysed copper (Cu), manganese (Mn), zinc (Zn) and cadmium (Cd) accumulation characteristics in Chinese turnips and the effect of soil physicochemical properties on both HM accumulation and translocation. Our results indicate that Chinese turnips absorb and translocate Mn, Zn, and Cd at much higher levels than they do Cu. When we measured bioconcentration factors in Chinese turnips for different HMs in the same soil, we found Chinese turnip capacities for HM accumulation decrease from Zn > Mn > Cd > Cu. In addition, the translocation factor for these HMs decreases from Mn > Cd > Zn > Cu. Correlation analysis indicates that soil pH and various soil components are either negatively or positively correlated with Mn, Zn, and Cd accumulation; also, soil properties are correlated with Mn translocation from root to leaf. These findings may help evaluate HM accumulation and translocation mechanisms as well as artificially regulate HM uptake levels from soils to turnips. Keywords: Turnip, Heavy metal, Bioconcentration, Phytoremediation, Soil composition
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- 2019
7. Physiological and biochemical analysis of mechanisms underlying cadmium tolerance and accumulation in turnip
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Xiong Li, Boqun Li, Yongping Yang, Xiaoming Zhang, and Yuansheng Wu
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0106 biological sciences ,Antioxidant ,medicine.medical_treatment ,Plant Science ,Vacuole ,FW, fresh weight ,010501 environmental sciences ,01 natural sciences ,GST, glutathione S-transferase ,DW, dry weight ,Cd, cadmium ,PCs, phytochelatins ,lcsh:Botany ,GSH, glutathione ,GR, glutathione reductase ,Osmotic pressure ,lcsh:QH301-705.5 ,Antioxidant system ,chemistry.chemical_classification ,HM, heavy metal ,Cadmium ,food and beverages ,Phytochelatin ,Zn, zinc ,lcsh:QK1-989 ,Amino acid ,Biochemistry ,POD, peroxidase ,APX, ascorbate peroxidase ,H2O2, hydrogen peroxide ,Detoxification ,O2-, superoxide anion ,chemistry.chemical_element ,DHAR, dehydroascorbate reductase ,TCA, trichloroacetic acid ,Article ,ROS, reactive oxygen species ,SOD, superoxide dismutase ,medicine ,Turnip ,Hyperaccumulator ,Ecology, Evolution, Behavior and Systematics ,0105 earth and related environmental sciences ,MDA, malondialdehyde ,Reactive oxygen species ,lcsh:Biology (General) ,chemistry ,Ni, nickel ,AsA, ascorbic acid ,CAT, catalase ,010606 plant biology & botany - Abstract
The capacity of plants to accumulate cadmium (Cd) is significant for phytoremediation of Cd-polluted soils. Turnips cultivated in China include species featuring high Cd accumulation and some of these plants act as Cd hyperaccumulator landraces. These plants can accumulate over 100 mg Cd kg −1 dry weight in leaves without injury. Hence, studies that explore mechanisms underlying Cd detoxification and transport in turnip plants are essential. In the present study, we compared physiological and biochemical changes in turnip leaves treated with two Cd concentrations to controls. We discovered that Cd stress significantly increased the enzymatic activities or compound contents in the antioxidant system, including members of the glutathione-ascorbic acid cycle, whereas oxidation of reactive oxygen species (ROS) remained stable. Cd treatments also increased the contents of phytochelatins as well as a number of amino acids. Based on these results, we conclude that turnips initiate a series of response processes to manage Cd treatment. First, the antioxidant system maintaining ROS homeostasis and osmotic adjustment is excited to maintain stability of cell osmotic potential. Cd is chelated into its stable form to reduce its toxicity. Cd is possibly transported to vacuoles or non-protoplasts for isolation. Amino acid synthesis may directly and indirectly play an important role in these processes. This study partly revealed physiological and biochemical mechanisms underlying turnip response to Cd stress and provides information on artificially increasing or decreasing Cd accumulation in turnips and other plants.
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- 2018
8. Evaluation of resistance of current wheat cultivars and breeding lines to stripe rust from three Gorges reservoir area
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Boqun Li, Yang Yu, Renwu Ruan, Fajing Chen, Chaowei Bi, Dejun Han, and Yuheng Yang
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0106 biological sciences ,0301 basic medicine ,Resistance (ecology) ,business.industry ,food and beverages ,Stripe rust ,Plant Science ,Biology ,biology.organism_classification ,01 natural sciences ,03 medical and health sciences ,030104 developmental biology ,Agronomy ,Agriculture ,Seedling ,Cultivar ,Low resistance ,business ,Agronomy and Crop Science ,010606 plant biology & botany ,Three gorges - Abstract
Nearly 100,000 ha in the Three Gorges Reservoir Area (TGRA) are in wheat production and the area is a junction where wheat stripe rust overwinters and causes epidemics the next spring; thus the area plays a pivotal role in wheat stripe rust epidemics in China. To better understand wheat resistance levels and the application of Yr genes in this area, 116 wheat cultivars (lines) were collected from the TGRA to investigate stripe rust resistance during the 2014–2016 cropping seasons. Seedling resistance evaluation results indicated that only nine accessions (7.8%) were immune or nearly immune to three predominant races of CYR32, CYR33 and PST-V26. In the field evaluation, 51 accessions (43.9%) showed adult-plant resistance, whereas 56 accessions (48.3%) were susceptible. The application of resistant sources focused on ineffective Yr9 (26.7%) and Yr17 (18.9%), and gradual ineffective Yr26 (34.5%), while effective Yr5, Yr10 and Yr15 were absent. Among them, 21 accessions (18.1%) were combined with two resistance genes. Both low resistance and more concentrated use of Yr genes indicated that this region faces a major risk for a wheat stripe rust epidemic. To improve the wheat resistance level in the TGRA, it is important to discover new all-stage resistance resources and diversify resistance resources for breeding.
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- 2017
9. Comparative expression analysis of heavy metal ATPase subfamily genes between Cd-tolerant and Cd-sensitive turnip landraces
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Yonghong Yang, Yuansheng Wu, Xi Han, Di Chen, Yongping Yang, Xiong Li, and Boqun Li
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0106 biological sciences ,Subfamily ,pI, isoelectric point ,Plant Science ,Biology ,SSI, seedling survival index ,010603 evolutionary biology ,01 natural sciences ,Genome ,Subclass ,Article ,MW, molecular weight ,Phylogenetics ,lcsh:Botany ,Coding region ,Turnip ,HMA, heavy metal ATPase ,lcsh:QH301-705.5 ,Gene ,Differential gene expression ,Ecology, Evolution, Behavior and Systematics ,Heavy metal ATPase ,Genetics ,HM, heavy metal ,Phylogenetic tree ,Chromosome ,food and beverages ,GPI, germination percentage index ,lcsh:QK1-989 ,CK, control blank ,GRAVY, grand average of hydropathicity ,lcsh:Biology (General) ,Transmembrane helix ,010606 plant biology & botany ,Cadmium - Abstract
The heavy metal ATPase (HMA) subfamily is mainly involved in heavy metal (HM) tolerance and transport in plants, but an understanding of the definite roles and mechanisms of most HMA members are still limited. In the present study, we identified 14 candidate HMA genes named BrrHMA1–BrrHMA8 from the turnip genome and analyzed the phylogeny, gene structure, chromosome distribution, and conserved domains and motifs of HMAs in turnip (Brassica rapa var. rapa). According to our phylogenetic tree, the BrrHMAs are divided into a Zn/Cd/Co/Pb subclass and Cu/Ag subclass. The BrrHMA members show similar structural characteristics within subclasses. To explore the roles of BrrHMAs in turnip, we compared the gene sequences and expression patterns of the BrrHMA genes between a Cd-tolerant landrace and a Cd-sensitive landrace. Most BrrHMA genes showed similar spatial expression patterns in both Cd-tolerant and Cd-sensitive turnip landraces; some BrrHMA genes, however, were differentially expressed in specific tissue in Cd-tolerant and Cd-sensitive turnip. Specifically, BrrHMA genes in the Zn/Cd/Co/Pb subclass shared the same coding sequence but were differentially expressed in Cd-tolerant and Cd-sensitive turnip landraces under Cd stress. Our findings suggest that the stable expression and up-regulated expression of BrrHMA Zn/Cd/Co/Pb subclass genes under Cd stress may contribute to the higher Cd tolerance of turnip landraces. Keywords: Heavy metal ATPase, Cadmium, Turnip, Transmembrane helix, Differential gene expression
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- 2018
10. Selenium Accumulation Characteristics and Biofortification Potentiality in Turnip (Brassica rapa var. rapa) Supplied with Selenite or Selenate
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Xiong Li, Yuansheng Wu, Boqun Li, Yonghong Yang, and Yongping Yang
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0106 biological sciences ,0301 basic medicine ,turnip ,selenate ,Biofortification ,chemistry.chemical_element ,Bioconcentration ,Plant Science ,lcsh:Plant culture ,engineering.material ,tibetan plateau ,01 natural sciences ,Selenate ,03 medical and health sciences ,chemistry.chemical_compound ,Animal science ,Selenium deficiency ,medicine ,lcsh:SB1-1110 ,Dry matter ,selenium deficiency ,medicine.disease ,Indicator plant ,030104 developmental biology ,chemistry ,engineering ,Fertilizer ,selenite ,Selenium ,010606 plant biology & botany - Abstract
Selenium (Se) is an essential trace element for humans. About 70% of the regions in China, including most of the Tibetan Plateau, are faced with Se deficiency problems. Turnip is mainly distributed around the Tibetan Plateau and is one of the few local crops. In the present study, we compared the absorption and translocation differences of Se (IV) selenite and Se (VI) selenate in turnip. The results showed that Se treatment, either by soil addition (0.2–2 mg Se kg−1 dry soil) or by foliar spraying (50–200 mg L−1 Se), could significantly increase the Se concentrations in turnips, and 0.5 mg Se (IV) or Se (VI) kg−1 dry matter in soils could improve the biomasses of turnips. Moreover, turnip absorbed significantly more Se (VI) than Se (IV) at the same concentration and also transferred much more Se (VI) from roots to leaves. Based on the Se concentrations, as well as the bioconcentration factors and translocation coefficients, we considered that turnip might be a potential Se indicator plant. Subsequently, we estimated the daily Se intake for adults based on the Se concentrations in turnip roots. The results indicated that Se (IV) should be more suitable as an artificial Se fertilizer for turnips, although the levels found in most samples in this study could cause selenosis to humans. In addition, we also estimated the optimum and maximum Se concentrations for treating turnips based on the linear relations between Se concentrations in turnip roots and Se treatment concentrations. The results provided preliminary and useful information about Se biofortification in turnips.
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- 2018
11. Selenium Accumulation Characteristics and Biofortification Potentiality in Turnip (
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Xiong, Li, Yuansheng, Wu, Boqun, Li, Yonghong, Yang, and Yongping, Yang
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turnip ,selenate ,selenium deficiency ,Plant Science ,selenite ,tibetan plateau ,Original Research - Abstract
Selenium (Se) is an essential trace element for humans. About 70% of the regions in China, including most of the Tibetan Plateau, are faced with Se deficiency problems. Turnip is mainly distributed around the Tibetan Plateau and is one of the few local crops. In the present study, we compared the absorption and translocation differences of Se (IV) selenite and Se (VI) selenate in turnip. The results showed that Se treatment, either by soil addition (0.2–2 mg Se kg−1 dry soil) or by foliar spraying (50–200 mg L−1 Se), could significantly increase the Se concentrations in turnips, and 0.5 mg Se (IV) or Se (VI) kg−1 dry matter in soils could improve the biomasses of turnips. Moreover, turnip absorbed significantly more Se (VI) than Se (IV) at the same concentration and also transferred much more Se (VI) from roots to leaves. Based on the Se concentrations, as well as the bioconcentration factors and translocation coefficients, we considered that turnip might be a potential Se indicator plant. Subsequently, we estimated the daily Se intake for adults based on the Se concentrations in turnip roots. The results indicated that Se (IV) should be more suitable as an artificial Se fertilizer for turnips, although the levels found in most samples in this study could cause selenosis to humans. In addition, we also estimated the optimum and maximum Se concentrations for treating turnips based on the linear relations between Se concentrations in turnip roots and Se treatment concentrations. The results provided preliminary and useful information about Se biofortification in turnips.
- Published
- 2017
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