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3 results on '"Maserti, Bianca Elena"'

1. Salt-stress induced changes in the leaf proteome of diploid and tetraploid mandarins with contrasting Na+ and Cl- accumulation behaviour.

Author

Podda A, Checcucci G, Mouhaya W, Centeno D, Rofidal V, Del Carratore R, Luro F, Morillon R, Ollitrault P, and Maserti BE

Subjects
Chlorides metabolism, Citrus drug effects, Sodium metabolism, Citrus metabolism, Diploidy, Plant Leaves drug effects, Plant Leaves metabolism, Proteome metabolism, Sodium Chloride pharmacology, Tetraploidy
Abstract

To understand the genotypic variation of citrus to mild salt stress, a proteomic approach has been carried out in parallel on two citrus genotypes ('Cleopatra' and 'Willow leaf' mandarins), which differ for Na(+) and Cl(-) accumulation, and their cognate autotetraploids (4×). Using two-dimensional electrophoresis approximately 910 protein spots were reproducibly detected in control and salt-stressed leaves of all genotypes. Among them, 44 protein spots showing significant variations at least in one genotype were subjected to mass spectrometry analysis for identification. Salt-responsive proteins were involved in several functions, including photosynthetic processes, ROS scavenging, stress defence, and signalling. Genotype factors affect the salt-responsive pattern, especially that of carbon metabolism. The no ion accumulator 'Cleopatra' mandarin genotype showed the highest number of salt-responsive proteins, and up-regulation of Calvin cycle-related proteins. Conversely the ion accumulator 'Willow leaf' mandarin showed high levels of several photorespiration-related enzymes. A common set of proteins (twelve spots) displayed higher levels in salt-stressed leaves of 2× and 4× 'Cleopatra' and 4× 'Willow leaf' mandarin. Interestingly, antioxidant enzymes and heat shock proteins showed higher constitutive levels in 4× 'Cleopatra' mandarin and 4× 'Willow leaf' mandarin compared with the cognate 2× genotype. This work provides for the first time information on the effect of 8 weeks of salt stress on citrus genotypes contrasting for ion accumulation and their cognate autotetraploids. Results underline that genetic factors have a predominant effect on the salt response, although a common stress response independent from genotype was also found., (Copyright © 2013 Elsevier GmbH. All rights reserved.)

Published
2013
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2. A stress responsive alternative splicing mechanism in Citrus clementina leaves.

Author

Del Carratore R, Magaldi E, Podda A, Beffy P, Migheli Q, and Maserti BE

Subjects
Acetates pharmacology, Amino Acid Sequence, Animals, Base Sequence, Chitinases genetics, Citrus drug effects, Citrus parasitology, Cyclopentanes pharmacology, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Plant, Molecular Sequence Data, Oxylipins pharmacology, Plant Leaves drug effects, Plant Leaves parasitology, Plant Proteins genetics, RNA, Messenger metabolism, Stress, Physiological, Tetranychidae physiology, Alternative Splicing, Chitinases metabolism, Citrus enzymology, Plant Leaves enzymology, Plant Proteins metabolism, RNA, Messenger drug effects
Abstract

Chitinases are often considered pathogenesis-related proteins since their activity can be induced by viral infections, fungal and bacterial cell wall components, and also by more general sources of stress such as wounding, salicylic acid, ethylene, auxins and cytokinins. In the present study, comparative proteomic analysis showed the defense-related acidic chitinase II to be specifically induced in Citrus clementina leaves infested by the two-spotted spider mite Tetranychus urticae or treated with MeJA. In parallel, changes in the mRNA profiles of two partially homologous chitinase forms were shown by RT-PCR. In particular, the appearance of an additional cDNA chitinase fragment in T. urticae-infested and MeJA-treated leaves was observed. This finding may indicate a specific regulatory mechanism of chitinase expression. We report evidence for alternative splicing in T. urticae-infested C. clementina, where a premature stop codon after the first 135 amino acids was introduced. We observed inducible chitinase activity after MeJA treatment, indicative of a rapid plant response to infestation. This work provides the first evidence of chitinase alternative splicing in C. clementina. In addition, the presence of the dual-band pattern for chitinase cDNA by RT-PCR may represent a suitable predictive marker for early diagnosis of plant biotic stress., (Copyright © 2011 Elsevier GmbH. All rights reserved.)

Published
2011
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3. Characterization and Expression of DNA Sequences Encoding Putative Type-II Metallothioneins in the Seagrass Posidonia oceanica1

Author

Giordani, Tommaso, Natali, Lucia, Maserti, Bianca Elena, Taddei, Sonia, and Cavallini, Andrea

Subjects
DNA, Plant, Molecular Sequence Data, Mercury, Blotting, Northern, Polymerase Chain Reaction, Trace Elements, Plant Leaves, Blotting, Southern, Magnoliopsida, Metallothionein, Amino Acid Sequence, RNA, Messenger, Sequence Alignment, Pseudogenes, Research Article
Abstract

Posidonia oceanica is a marine phanerogam, largely widespread in the Mediterranean sea, representing an important food substrate for many marine organisms. A progressive reduction of P. oceanica meadows has been reported, due to anthropogenic coastal activity. Studying mechanisms by which this species responds to environmental stresses, three DNA sequences putatively encoding metallothioneins (MTs) have been isolated, by PCR. Two sequences, Pomt2a (accession no. AJ249603) and Pomt2b (accession no. AJ249602), show high similarities with genes encoding type-II MTs and are interrupted by two and one intron, respectively. The third sequence, Pomt2c (accession no. AJ249604), is supposed to be a pseudogene, originated by retrotranscription of the Pomt2b mRNA. These sequences belong to a multigene family with at least five members. Northern hybridizations indicated that MT transcripts accumulation is constitutive and seasonally regulated. MT encoding RNAs increase after rhyzome harvesting and (at a lesser extent) after 15 d of cultivation in an aquarium. As for animal MTs, transcripts accumulation is observed also after exposure to trace metals such as copper and cadmium. In the case of copper, the effect depends on concentration. Finally, taking into consideration the great interest in studying the biogeochemical cycle of mercury in the Mediterranean basin and since P. oceanica is commonly considered a bioindicator of this metal, the effect of mercury treatments on the accumulation of MT transcripts has been analyzed: in only a few experiments a small increase in the level of transcripts was recorded, suggesting that MTs are not key elements in the mercury accumulation by this species.

Published
2000

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