1. Optimization and validation of extraction and quantification methods of antimalarial triterpenic esters in Keetia leucantha plant and plasma.
- Author
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Beaufay C, Henry G, Streel C, Bony E, Hérent MF, Bero J, and Quetin-Leclercq J
- Subjects
- Humans, Linear Models, Reproducibility of Results, Sensitivity and Specificity, Antimalarials analysis, Antimalarials blood, Antimalarials isolation & purification, Plant Extracts chemistry, Rubiaceae chemistry, Triterpenes analysis, Triterpenes blood, Triterpenes isolation & purification
- Abstract
The aim of this study is to develop validated methods for the extraction and quantification of antimalarial triterpene esters from Keetia leucantha and from plasma samples. These compounds, showing in vitro and in vivo antiplasmodial activities, were optimally extracted from Keetia leucantha twigs using ultrasounds with dichloromethane and from plasma using protein precipitation with acetonitrile. We then developed and validated HPLC-UV quantification methods, which proved to be selective, accurate, linear, true and precise, both in plant and plasma samples for the eight triterpenic esters in mixture. Based on the total error concept as decision criteria, the validated dosage ranges of the triterpene esters mixture were set between 14.68 and 73.37 μg/mL in plants and 15.90 and 106.01 μg/mL in plasma injected solutions, corresponding to 7.95 and 53.01 μg/mL in plasma. These reliable methods were used to determine effectively triterpene esters content in collected samples, that seems highly variable in plant extracts, and will be helpful to further investigate pharmacokinetics parameters of these interesting bioactive compounds., (Copyright © 2018 Elsevier B.V. All rights reserved.)
- Published
- 2019
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