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Your search keyword '"Abete, Lorena"' showing total 3 results
Start Over Author "Abete, Lorena" Topic plant extracts
3 results on '"Abete, Lorena"'

1. A multi-methodological approach in the study of Italian PDO "Cornetto di Pontecorvo" red sweet pepper.

Author

Sobolev AP, Mannina L, Capitani D, Sanzò G, Ingallina C, Botta B, Fornarini S, Crestoni ME, Chiavarino B, Carradori S, Locatelli M, Giusti AM, Simonetti G, Vinci G, Preti R, Toniolo C, Reverberi M, Scarpari M, Parroni A, Abete L, Natella F, and Di Sotto A

Subjects
Antifungal Agents pharmacology, Biogenic Amines analysis, Capsicum metabolism, Chromatography, High Pressure Liquid, Flavonoids analysis, Food Quality, Fruit chemistry, Magnetic Resonance Spectroscopy, Mass Spectrometry, Mycotoxins analysis, Polyphenols analysis, Seeds chemistry, Tannins analysis, alpha-Amylases antagonists & inhibitors, Capsicum chemistry, Food Analysis methods, Plant Extracts pharmacology
Abstract

A multi-methodological approach was applied to study red sweet peppers (Capsicum annuum L.) ecotype "Cornetto di Pontecorvo" grown in a greenhouse or in open field. This approach includes morphological analysis, chemical composition determination, and biological activity evaluation of different extracts from pepper fruits. Untargeted analyses, namely NMR spectroscopy and mass spectrometry, allowed the comprehensive pepper metabolite profile of pepper pulp, peel and seeds hydroalcoholic and organic extracts to be determined, showing the presence of sugars, organic acids, amino acids and other secondary metabolites. Targeted analyses, such as HPLC-PDA, HPLC-TLC and spectrophotometric analyses allowed polyphenols, tannins, flavonoids and pigments content to be determined. Samples quality and freshness were verified by the low content of biogenic amines and mycotoxins, as determined using HPLC-FLD and HPLC-MS, respectively. Preliminary biological results demonstrated the ability of the organic extracts to inhibit α-amylase, a key enzyme in the control of glucose metabolism., (Copyright © 2018 Elsevier Ltd. All rights reserved.)

Published
2018
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2. Genotoxicity assessment of piperitenone oxide: An in vitro and in silico evaluation.

Author

Di Sotto A, Di Giacomo S, Abete L, Božović M, Parisi OA, Barile F, Vitalone A, Izzo AA, Ragno R, and Mazzanti G

Subjects
Cell Survival drug effects, Comet Assay, Computer Simulation, DNA Damage drug effects, Hep G2 Cells, Humans, Mentha chemistry, Micronucleus Tests, Monoterpenes chemistry, Mutagenicity Tests, Mutation drug effects, Oxides chemistry, Plant Extracts chemistry, Salmonella typhimurium drug effects, Salmonella typhimurium genetics, Monoterpenes toxicity, Oxides toxicity, Plant Extracts toxicity
Abstract

Piperitenone oxide, a natural flavouring agent also known as rotundifolone, has been studied for the genotoxicity assessment by an integrated in vitro and in silico experimental approach, including the bacterial reverse mutation assay, the micronucleus test, the comet assay and the computational prediction by Toxtree and VEGA tools. Under our experimental conditions, the monoterpene showed to induce both point mutations (i.e. frameshift, base-substitution and/or oxidative damage) and DNA damage (i.e. clastogenic or aneuploidic damage, or single-strand breaks). Computational prediction for piperitenone oxide agreed with the toxicological data, and highlighted the presence of the epoxide function and the α,β-unsaturated carbonyl as possible structural alerts for DNA damage. However, improving the toxicological libraries for natural occurring compounds is required in order to favour the applicability of in silico models to the toxicological predictions. Further in vivo evaluations are strictly needed in order to evaluate the role of the bioavailability of the substance and the metabolic fate on its genotoxicity profile. To the best of our knowledge, these data represent the first evaluation of the genotoxicity for this flavour compound and suggest the need of further studies to assess the safety of piperitenone oxide as a flavouring agent., (Copyright © 2017 Elsevier Ltd. All rights reserved.)

Published
2017
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3. Genotoxicity assessment of piperitenone oxide: An in vitro and in silico evaluation

Author

Antonella Di Sotto, Lorena Abete, Mijat Božović, Olga A. Parisi, Rino Ragno, Annabella Vitalone, Fabio Barile, Gabriela Mazzanti, Angelo A. Izzo, Silvia Di Giacomo, Di Sotto, Antonella, Di Giacomo, Silvia, Abete, Lorena, Božović, Mijat, Parisi, OLGA ALESSANDRA, Barile, Fabio, Vitalone, Annabella, Izzo, ANGELO ANTONIO, Ragno, Rino, and Mazzanti, Gabriela

Subjects
0301 basic medicine, mentha spp, Salmonella typhimurium, DNA damage, Cell Survival, In silico, point mutations, Mentha spp, 010501 environmental sciences, Pharmacology, Biology, Toxicology, medicine.disease_cause, 01 natural sciences, 03 medical and health sciences, Clastogen, medicine, Humans, Computer Simulation, 0105 earth and related environmental sciences, chromosomal damage, flavouring agent, genotoxicity assessment, In silico prediction, rotundifolone, Point mutation, Micronucleus Tests, Mutagenicity Tests, Plant Extracts, Oxides, General Medicine, Hep G2 Cells, Bioavailability, Comet assay, 030104 developmental biology, Biochemistry, Micronucleus test, Mutation, Monoterpenes, Comet Assay, Function (biology), Genotoxicity, Food Science, DNA Damage, Mentha
Abstract

Piperitenone oxide, a natural flavouring agent also known as rotundifolone, has been studied for the genotoxicity assessment by an integrated in vitro and in silico experimental approach, including the bacterial reverse mutation assay, the micronucleus test, the comet assay and the computational prediction by Toxtree and VEGA tools. Under our experimental conditions, the monoterpene showed to induce both point mutations (i.e. frameshift, base-substitution and/or oxidative damage) and DNA damage (i.e. clastogenic or aneuploidic damage, or single-strand breaks). Computational prediction for piperitenone oxide agreed with the toxicological data, and highlighted the presence of the epoxide function and the α,β-unsaturated carbonyl as possible structural alerts for DNA damage. However, improving the toxicological libraries for natural occurring compounds is required in order to favour the applicability of in silico models to the toxicological predictions. Further in vivo evaluations are strictly needed in order to evaluate the role of the bioavailability of the substance and the metabolic fate on its genotoxicity profile. To the best of our knowledge, these data represent the first evaluation of the genotoxicity for this flavour compound and suggest the need of further studies to assess the safety of piperitenone oxide as a flavouring agent.

Published
2017

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