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1. Deciphering the role of the phenylpropanoid metabolism in the tolerance of Capsicum annuum L. to Verticillium dahliae Kleb.

Author

Novo M, Silvar C, Merino F, Martínez-Cortés T, Lu F, Ralph J, and Pomar F

Subjects
Capsicum metabolism, Cell Wall metabolism, Coumaric Acids metabolism, Lignin metabolism, Phenols metabolism, Tyramine analogs & derivatives, Tyramine metabolism, Capsicum microbiology, Plant Diseases microbiology, Propanols metabolism, Verticillium pathogenicity
Abstract

Verticillium dahliae is an economically relevant soilborne pathogen that causes vascular wilt in several crops, including pepper (Capsicum annuum). Fungal infection is usually visualized as a vascular browning, likely due to the onset of phenylpropanoid metabolism, which also seems to play a crucial role in the tolerance of some pepper varieties. In the current work, the potential function of distinct phenylpropanoid derivatives (suberin, lignin and phenolic compounds) in the pepper tolerance response against V. dahliae, was investigated. Histochemical and biochemical analyses ruled out suberin as a key player in the pepper-fungus interaction. However, changes observed in lignin composition and higher deposition of bound phenolics in infected stems seemed to contribute to the reinforcement of cell walls and the impairment of V. dahliae colonization. Most importantly, this is the first time that the accumulation of the hydroxycinnamic acid amide N-feruloyltyramine was reported in pepper stems in response to a vascular fungus. Fungitoxic activity for that hydroxycinnamate-tyramine conjugate was demonstrated as well., (Copyright © 2017 Elsevier B.V. All rights reserved.)

Published
2017
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2. Comparative proteomics of extracellular proteins in vitro and in planta from the pathogenic fungus Fusarium graminearum

Author

Neil D. Adhikari, Janet M. Paper, John S. Scott-Craig, Jonathan D. Walton, and Christina A. Cuomo

Subjects
Signal peptide, Proteomics, Proteome, Genes, Fungal, In Vitro Techniques, Biochemistry, Aconitase, Models, Biological, Chromosomes, Plant, Mass Spectrometry, Microbiology, Fungal Proteins, Fusarium, Trypsin, Molecular Biology, Triticum, Plant Diseases, Plant Proteins, biology, fungi, food and beverages, Chromosome Mapping, biochemical phenomena, metabolism, and nutrition, Pathogenic fungus, biology.organism_classification, In vitro, Gibberella zeae, Secretory protein, Phosphoglucomutase
Abstract

High-throughput MS/MS was used to identify proteins secreted by Fusarium graminearum (Gibberella zeae) during growth on 13 media in vitro and in planta during infection of wheat heads. In vitro secreted proteins were collected from the culture filtrates, and in planta proteins were collected by vacuum infiltration. A total of 289 proteins (229 in vitro and 120 in planta) were identified with high statistical confidence. Forty-nine of the in planta proteins were not found in any of the in vitro conditions. The majority (91-100%) of the in vitro proteins had predicted signal peptides, but only 56% of the in planta proteins. At least 13 of the nonsecreted proteins found only in planta were single-copy housekeeping enzymes, including enolase, triose phosphate isomerase, phosphoglucomutase, calmodulin, aconitase, and malate dehydrogenase. The presence of these proteins in the in planta but not in vitro secretome might indicate that significant fungal lysis occurs during pathogenesis. On the other hand, several of the proteins lacking signal peptides that were found in planta have been reported to be potent immunogens secreted by animal pathogenic fungi, and therefore could be important in the interaction between F. graminearum and its host plants.

Published
2007