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This article discusses the engineering of soybean plants with high levels of herbicide resistance using a Cas12-SF01-based cytosine base editor. Non-transgenic herbicide-tolerant soybean varieties are in demand due to the challenges posed by weeds in soybean cultivation. The study explores the use of amino acid substitution mutations in multiple ALS genes to produce soybeans with sufficient herbicide resistance. The researchers successfully generated herbicide-resistant soybean mutants and evaluated their resistance in greenhouse and field experiments. The results showed that the mutants exhibited strong herbicide resistance without affecting plant productivity. This study demonstrates the effectiveness of the base editor system for breeding herbicide-tolerant soybeans. [Extracted from the article]

A study published in the Journal of Integrative Plant Biology explores the use of CRISPR/Cas12i3 gene editing to enhance the aroma of soybeans. By knocking out the genes GmBADH1 and GmBADH2, researchers were able to increase the production of 2‐Acetyl‐1‐pyrroline (2‐AP), a compound responsible for the pleasant fragrance in crops. Soy milk made from the mutant seeds exhibited a much stronger aroma, which was preferred by consumers. This research has the potential to improve the taste quality of soy-based products. [Extracted from the article]

A new base editor CGBE with a uracil DNA N-glycosylase (UNG) has recently been reported that enables efficient C-to-G editing in mammalian cells (Kurt I et al i ., 2021). Keywords: Cas9; base editing; rice; CGBE; UNG EN Cas9 base editing rice CGBE UNG 1238 1240 3 07/01/22 20220701 NES 220701 Cytosine and adenosine base editors (CBE and ABE) have been vigorously developed in plants, but the base conversion types are limited (Ren I et al i ., 2018, 2021; Xu I et al i ., 2021). Efficient C-to-G editing in rice using an optimized base editor Protoplasts-based assays revealed a substantial increase in the C-to-G/A editing efficiency compared with CBE (0.49% vs 0.12%), indicating C-to-G/A editing feasibility (Figure 1c). [Extracted from the article]