1. Expression of p57 KIP2 reduces growth and invasion, and induces syncytialization in a human placental choriocarcinoma cell line, BeWo.
- Author
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Takahashi K, Yoneyama Y, Koizumi N, Utoguchi N, Kanayama N, and Higashi N
- Subjects
- Cell Differentiation physiology, Cell Line, Tumor, Cyclin-Dependent Kinase Inhibitor p57 genetics, Female, Humans, Placenta pathology, Pregnancy, Cell Proliferation physiology, Cyclin-Dependent Kinase Inhibitor p57 metabolism, Placenta metabolism, Trophoblasts metabolism
- Abstract
Introduction: Syncytiotrophoblasts are the major components of the human placenta involved in fetal maternal exchange and hormone secretion. The syncytiotrophoblasts arise from the fusion of villous cytotrophoblasts. The cell cycle suppressor p57
KIP2 is known to be an essential molecule for proper trophoblast differentiation during placental formation., Methods: We generated p57KIP2 -expressing BeWo transfectant cells. Proliferation assay and matrigel invasion assay were used to characterize p57KIP2 -expressing BeWo transfectant cells. To reveal the role of p57KIP2 in syncytialization, we proceeded syncytium formation analysis and qRT-PCR for detection of the expression levels Syncytin-1, Syncytin-2 and their receptors., Results: The human choriocarcinoma cell line, BeWo has undetectable levels of p57KIP2 expression. Expression of p57KIP2 reduced cell proliferation rate and extracellular matrix invasion activity. p57KIP2 expressing cells displayed multinucleated cells associated with syncytiotrophoblast differentiation. In the syncytialization event, p57KIP2 was found to potentiate forskolin-induced upregulation of Syncytin-2 in a cAMP-independent manner., Discussion: These results indicate that the expression of p57KIP2 may act on the proliferation/invasion inhibitory factor and enhance the expression of Syncytin-2, which are associated with syncytialization in cytotrophoblasts., (Copyright © 2020 Elsevier Ltd. All rights reserved.)- Published
- 2021
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