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The development of transgenic technologies in the Cashmere goat ( Capra hircus ) has the potential to improve the quality of the meat and wool. The piggyBac (PB) transposon system is highly efficient and can be used to transpose specific target genes into the genome. Here, we developed a PB transposon system to produce transgenic Cashmere goat fetal fibroblasts (GFFs) with the enhanced green fluorescent protein (EGFP). We then used the genetically modified GFFs as nuclear donors to generate transgenic embryos by somatic cell nuclear transfer (SCNT). The embryos (n = 40) were implanted into female goats (n = 20). One transgenic kid that expressed EGFP throughout the surface features of its body was born. This result demonstrated the usefulness of PB transposon system in generating transgenic Cashmere goats. [ABSTRACT FROM AUTHOR]

The article discusses application of PiggyBac (PB) specific transposon mediated gene transfer in fetal fibroblast cells of Cashmere goats. It reports that PB mediated gene transfer increases the efficiency of goat fetal fibroblasts (GFFs) cells that was determined through increased EGFP gene expression levels. The function of PB transposon in GFFs that provides potential tool for production of transgenic goats is also included.