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Introduction: The healing is a complex biological event that involves inflammation, chemotaxis, cells proliferation, differentiation and remodeling. In Brazilian popular medicine, the "Aroeira" is used to treat different situations The anastomotic healing is always a preoccupation among surgeons. The fail in intestinal anastomosis leads to fistulas and dehiscences., Purpose: To evaluate the effect of the hydroalcoholic extract of Schinus terebinthifolius Raddi (Brazilian pepper tree) in the healing of colonic anastomosis., Methods: Forty Wistar rats were randomly divided in two groups (Aroeira and control), each one with 20 animals, according to the treatment received after the anastomosis (Aroeira extract or saline solution 0,9%) and each group was divided in two subgroups (C3 and C7 and A3 and A7,) according to the euthanasia day (at 3rd and at 7th). The macroscopic, microscopic and bursting pressure measurements were performed. To evaluate the groups, the non-parametric test of Mann-Whitney was applied., Results: In the macroscopic aspects, there was no significant difference between the Aroeira and the control group, both at the 3rd and the 7th days. When compared the subgroups of microscopic analysis at the 3rd day, the difference between the Aroeira and the control group was significant in the variables congestion (p = 0.005), polymorphonuclears (p = 0.034), mononuclears (p = 0.023), fibroblastic proliferation (p = 0.023) and at the healing stage (p = 0.001). At the 7th day analysis, the difference between the Aroeira and the control group was significant in all the variables of microscopic analysis. No significant differences were found in the bursting pressure., Conclusion: It was observed a favorable effect of Aroeira, at microscopic level, in the healing process of colonic anastomosis.

Introduction: The Brazilian popular habit of using plants to treat several health conditions is ancient. Passion fruit (Passiflora edulis) is widely used to treat, usually in an empiric basis, a variety of medical conditions. Anti-inflammatory activity of Passiflora edulis extract, similar to non-steroidal anti-inflammatory drugs (NSAID's), has been described., Purpose: To evaluate the effect of Passiflora edulis hydroalcoholic extract on the healing of midline abdominal incisions in rats by morphological and tensiometric methods., Methods: Forty male Wistar rats were randomly allocated into two groups to either receive Passiflora edulis extract (study group, P) or saline (control group, C) intraperitoneally, in a single isovolumetric dose, after a standardized ventral midline laparotomy had been performed. The twenty rats of the control group as well as those twenty of the study group were divided into subgroups according to the time of sacrifice, either the 3rd PO day (P3, C3) or the 7th PO day (P7, C7). On day three and on day seven after surgery, the rats were sacrificed and the wound area was excised by a standardized protocol. The healing process of the specimens was evaluated macroscopically and histologically. The tensile strength was evaluated by a constant speed computerized tensiometer to determine the breaking strength and the deformation of the healing incision., Results: The macroscopic examination did not show significant differences between study and control groups. Histologically, the C3 vs. P3 comparison showed the following differences: for the variables acute inflammation (p=0.045 in favor of C3), collagenization and capillary neoformation: p=0.001 e 0.001, respectively in favor of P3. Similarly, the C7 vs. P7 comparison showed the following differences for the variables acute inflammation (p=0.002 in favor of C7), chronic inflammation and capillary neoformation: p= 0.006 e 0.001, respectively in favor of P7. Tensiometrically, maximal breaking strength (C(máx)) on day seven of the study group was higher when compared to control group, (6.91 +/- 1.36 vs. 5.05 +/- 1.63, p=0.013). Maximal deformation strength (D(máx)) on day seven of the study group was higher when compared to control group (36.49 +/- 4.61 vs. 26.19 +/-5.74, p=0.001)., Conclusion: Passiflora edulis extract enhances the healing of midline abdominal incisions in rats, especially the histological and tensiometric aspects.

Introduction: The babassu mesocarp (Orbignya phalerata) has been used in experimental research studies focused on its antiinflammatory action. In state of Maranhão--Brazil it is widely used not only as food, but also as popular medicine in wound healing process., Purpose: To evaluate the action of Orbignya phalerata extract in macroscopic, histologic and tensiometric aspects in the healing process of median laparotomy in rats., Methods: Forty male adult Wistar rats were submitted to an incision in the alba linea, sutured back in one plan with separated stitches of polypropylene 5-0. After regular procedure, the animals were divided into two groups of 20 rats each. To the group named control an intraperitoneal, dose of 1.0 ml of saline solution per kilogram of body weight was done. To the experimental group, the same thing was also done, but instead of saline solution it was injected water solution of babassu, in a dose of 50 mg/kg. The animals were observed in the following days. All of them were killed within a three and seven day post-operative period schedule, and then a histological and tensiometric analysis was carried out., Results: On macroscopic examination no relevant adherence, between the alba linea and the abdominal organs in the study groups, was found. Histological evaluation presented marginal significant effects (p=0.86) to acute inflammation and significant effects (p=0.003) to giant cell reaction in both control and experimental three days groups. Significant difference was observed to acute inflammation in both seven days control and experimental groups. In the intragroup analysis (control three and seven) some marginal significant effect was in relationship to acute and chronic inflammation. In the inter-experimental groups analysis, only the giant cell reactions (0.002) and colagenization had significant results. The tensiometric evaluation showed in the seven day experimental group more resistance then others., Conclusion: The macroscopic and histological evaluation didn't show any significant difference between the experimental and control groups, but the tensiometric evaluation at the 7th day experimental group had significant difference compared to the control group, signaling that the use of the extract of babassu intraperitoneally injected can improve the healing process.

Introduction: In Brazilian countryside, cataplasm made from Passiflora edulis leaves has been used by the population as a healing agent for infections and skin inflammations in an empiric basis., Purpose: the aim of this work was to evaluate the healing process of open wounds in rats, in which Passiflora edulis hydro-alcoholic extract was applied., Methods: Sixty male, adult Wistar rats were divided into two groups: Passiflora group and Control group. Rats of the first group were treated with Passiflora edulis extract, and those of the second group received distilled water. The daily application of the extract or distilled water was carried out on a 2 cm diameter standardized circular wound on the dorsal region of each animal. Wound assessment was performed macroscopically and microscopically on the 7th, 14th, and 21st postoperative days. Microscopic analysis included hematoxylin-eosine and Masson Trichromium stains, evaluating inflammatory response, fibroplasia and collagen deposition. The wound retraction was evaluated by digital planimetry., Results: No significant difference in the rate of wound healing was detected comparing both groups. However, a significant increase in the number of fibroblastic cells was seen on the 7th PO day, and significantly greater collagen deposition was observed on the 14th day PO day in rats from the Passiflora group (p=0,012)., Conclusions: The application of the Passiflora edulis extract does not accelerate the healing process of open wounds in rats, but is associated with increased number of fibroblastic cells on 7th P.O. day and greater collagen deposition on the 14th PO day.

Introduction: The large use of plants in the prevention and treatment of different diseases is a milenar practice. In Brazil due to it's rich flora there is a huge material for research and use in popular medicine. The babassu (Orbignya phalerata) is a native tree from North of the Brazil and has a high concentration at the State of Maranhão. The powder of it's mesocarp is known as amid, and has been used as meal and medication due to the known activity against antiinflammatory reaction, pain, pyrexia and immunomodulation., Purpose: To analyze the effect of mesocarp of Orbignya phalerata (babassu) in gastric wound healing in rats under morphologic and tensiometric aspects., Method: Forty Wistar rats, male, adults were submitted to a longitudinal incision of 1 cm in the gastric body and sutured with separated stitches of polypropylene 6.0. After this common procedure the animals were allocated in the aleatory way in two groups Orbignya (GO) and Control (GC) with 20 animals each one. In the GO was used intraperitoneal doses of 50 mg/kh of Orbignya phalerata extract and in the GC water 1 ml/kg. Each group was divided in two subgroups of ten rats according to the period of death, at 3rd and 7th post-operative days. After the death, the abdominal cavity was evaluated and the stomach withdrawn. For the comparative analysis between the groups the macroscopic and histological parameters of wound healing were utilized., Results: There were no abscess, fistulae and haematom in the animals of the study. Abdominal adhesions were observed in both groups of the animals at 3rd and 7th post-operative days. Dehiscence of the gastrography was identified in one 3rd day rat of subgroups GO. The resistance to air insufflation was higher in GO 3rd day (p=0.087). The analysis of histological parameters showed statistical difference related to wound coaptation in favor to subgroup GO 7th post-operative day., Conclusions: The extract of mesocarp of Orbignya phalerata in dose and path utilized positively interfered on the gastrography when compared with control group in animals at 7th post-operative day.

Introduction: Phytotherapy has been drawing interest from the scientific community regarding its potential wound healing properties. There are few studies available that present a correct scientific methodology and, therefore, phytotherapy remains an open field for further research., Purpose: To evaluate the effects of Jatropha gossypiifolia L. (bellyache bush) on the healing of colonic anastomosis in rats., Methods: Forty Wistar rats were subjected to a complete section of the colon, that was soon sutured with eight separate stitches using monofilament polypropylene 6-0. Randomly, the animals were divided into control group (CG), with 20 rats, which were injected with 0.9% sodium chloride solution intraperitoneally (1 mL/kg) and Jatropha group (JG), also with 20 rats, which were injected with Jatropha gossypiifolia L. alcoholic extract intraperitoneally (1 mL/kg). Each of the groups was subdivided into two subgroups of ten rats, according to the sacrifice date, third and seventh days (CG3/ CG7 and JG3/ JG7). After sacrifice, the segment of the colon containing 1cm proximal and distal to the site of anastomosis was removed and subjected to the pressure test with air insulation. This segment was then opened and a 1.0 x 0.5 cm part was removed and placed under 10% formaldehyde for histological analysis with hematoxylin-eosin and Masson's trichrome stainings., Results: The evaluation of the pressure rupture test demonstrated statistical significance regarding the sacrifice date. On the third day, the average pressure of the CG (25.4 mmHg) and the JG (76.4 mmHg) revealed p = 0.013, and on the seventh day, the average pressure of the CG (187,3 mmHg.) and the JG (135.1 mmHg.) revealed p = 0.014. When assessing the microscopic variables among the groups of the 3rd day, only the variables edema and polymorphonuclear cells did not show statistical differences. On the 7th day, all of the variables assessed demonstrated significant differences., Conclusions: Regarding mechanical resistance, there was a difference in resistance between the JG3 and the CG3, and a shift of resistance when comparing the JG7 and CG7, which suggests a weakening of the Jatropha effect at a later stage of healing. The histological evaluation revealed an improvement of the acute inflammatory process in the JG3 when compared to the CG3, which was even more intense during the chronic stage, when the two groups are compared on the seventh day. The histological study of the inflammatory process, in general, showed a favorable effect to the Jatropha group compared to the control group.

Purpose: To evaluate the effects of hydroalcoholic extract of Passiflora edulis leaves in the healing of urinary bladder in rats from histological aspects., Methods: Forty Wistar male rats were submitted to a longitudinal incision of the bladder followed by a stetching in only one level. After this common procedure, animals were divided at random two groups: Passiflora and Control. In the Passiflora group the only dosage used was administered by intraperitoneal injection of hydroalcoholic extract of Passiflora edulis leaves while in the Control group distilled water was injected. Each subgroup was then divided in two subgroups according to the death of these animals: Control, three and seven days, Passiflora, three and seven days. After the death of these animals, an inventory of the abdominal cavity was performed and the bladder was removed. A comparative analysis was done between the two groups with microscopic evaluation of the healing. There was less acute inflammation (p=0.008), greater collagenous formation (p=0.001) and greater capillary neo-formation (p=0.000) in the third day Passiflora subgroup when compared to the Control subgroup of the third day., Results: There was less acute inflammation (p=0.001), greater fibroblastic proliferation (p=0.011) and greater collagenous formation (p=0.001) in the Passiflora subgroup of seventh day when compared with the Control seventh day subgroup., Conclusion: The use of Passiflora edulis leaves extract resulted in less acute inflammation, greater fibroblastic proliferation, collagenous formation and capillary neo-formation on rats' bladder wound healing.