1. Application of Bacillus megaterium MCR-8 improved phytoextraction and stress alleviation of nickel in Vinca rosea.
- Author
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Khan, Waheed Ullah, Ahmad, Sajid Rashid, Yasin, Nasim Ahmad, Ali, Aamir, Ahmad, Aqeel, and Akram, Waheed
- Subjects
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BACILLUS megaterium , *PHYTOREMEDIATION , *CATHARANTHUS roseus , *NICKEL in soils , *GAS exchange in plants - Abstract
The current research was performed to evaluate the effect ofBacillus megateriumMCR-8 on mitigation of nickel (Ni) stress inVinca roseagrown on Ni-contaminated soil (50, 100, and 200 mg Ni kg−1soil). The treated plants exhibited reduced growth, biomass, gas exchange capacity, and chlorophyll (Chl) content under Ni stress. The inoculated plants growing in Ni-contaminated media exhibited relatively higher growth, total soluble protein, and proline contents. Similarly, bacterial inoculation improved the activity of antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and ascorbate peroxidase (APX) under Ni stress. The Ni stress alleviation in inoculated plants was attributed to the reduced level of malondialdehyde (MDA) and hydrogen peroxide (H2O2), enhanced synthesis of protein, proline, phenols, and flavonides in conjunction with improved activity of antioxidant enzymes. The growth-promoting characteristics of microbe such as 1-aminocyclopropane-1-carboxylate deaminase (ACCD) and phosphate solubilization activity, siderophore, and auxin production capability also improved the growth and stress mitigation in inoculated plants. Furthermore, the inoculated plants exhibited higher value for bioconcentration factor (BCF), translocation factor (TF), and resulted in higher loss of Ni content from soil. The current results exhibited the beneficial role ofB. megateriumMCR-8 regarding stress alleviation and Ni phytoextraction byV. rosea. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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