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Photosynthesis is the most important bioenergy conversion process on Earth. Capturing instantaneous changes in in situ photosynthesis in open ocean ecosystems remains a major challenge. In this study, fast repetition rate fluorometry (FRRF), which can obtain nondestructive, real-time and in situ estimates of photosynthetic parameters, was used for the first time to continuously observe the spatial variation in in situ photosynthetic parameters in the eastern Indian Ocean (EIO). We further formulated new insights regarding abiotic and biotic factors of potential importance in determining photosynthetic performance. First, we found that the distributions of micro/nano- and picophytoplankton were opposite under the control of nutrient concentrations. Micro/nanophytoplankton had higher cell abundances in the nearshore and upwelling regions, while picophytoplankton had higher abundances in the open ocean, and Prochlorococcus was the dominant group. Second, based on the FRRF technology, we obtained the high-precision and high-density vertical profile map of photosynthetic parameters in the euphotic layer. It was observed that values of the maximum photochemical efficiency (Fv/Fm; 0.14-0.55, unitless) and the functional absorption cross-section of PSII (σ PSII ; 1.71-4.90 nm 2  RCII -1 ) increased with increasing depth, while high values of the photosynthetic electron transfer rates (ETR RCII ; 0.0019-17.0292 mol e -  mol RCII -1  s -1 ) and the nonphotochemical quenching (NPQ NSV ; 0.35-7.26, unitless) occurred in the shallow 50 m layer, and the values decreased as the depth increased. Finally, we discussed limiting factors that regulated the distribution of photosynthetic parameters and concluded that optical properties varied significantly with changes in the ocean physico-chemical parameters and taxonomic composition of phytoplankton assemblages in the EIO. Picophytoplankton (especially cyanobacteria), rather than the micro/nanophytoplankton community, was the dominant factor influencing photosynthesis. Among abiotic factors, photosynthetically active radiation (PAR) was the proximal limiting factor affecting photosynthetic efficiency, followed by temperature and dissolved inorganic nitrogen (DIN). Consequently, phytoplankton photosynthetic parameters exhibited great variability, allowing rapid responses to environmental condition changes. In this study, we established the basis for detecting future changes in primary production in this oligotrophic area., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

Statistical analysis of rainfall data from 2005 to 2015 showed that atmospheric deposition supplied large amount of dissolved inorganic nitrogen (38-155 mg·m -2 ·month -1 ) in N-deficient South China Sea and Eastern Indian Ocean. To understand marine ecosystem responses to increasing nutrient disturbances, we implemented field mesocosm experiments to study phytoplankton community structure and biodiversity responses to nutrient treatments with nitrate, phosphate and iron across tropical seas. Our results showed that DIN supply would change phytoplankton community structure and stimulated the regime shift from cyanobacteria to diatoms (relative dominance R > 0). Phytoplankton communities were dominated by diatoms (relative abundance >50%) accompanied by high chlorophyll a content with 1.58-39.27 μg·L -1 in DIN-added cultures, whereas cyanobacteria dominated communities (relative abundance >60%) with low biomass of 0.12-0.18 μg·L -1 in undisturbed cultures. Simultaneously increased DIN loading from atmospheric deposition would decrease ecological diversity of tropical seas owing to species competition and succession (Shannon diversity H' decreased to <1)., (Copyright © 2018 Elsevier Ltd. All rights reserved.)

The phytoplankton sinking rate in the eastern Indian Ocean was measured during spring 2017 based on the SETCOL method. The range of phytoplankton sinking rates was −0.291 to 2.188 md−1, with an average of 0.420 ± 0.646 md−1. The phytoplankton sinking rate in the Equator (EQ) and the eastern boundary of the Indian Ocean near Sumatra (EB) was lower than that in the Bay of Bengal (BOB). The sinking rate above 100 m was low and increased rapidly below 100 m in all the three regions. The phytoplankton community composition had an important impact on the phytoplankton sinking rate in the east Indian Ocean. The strong stratification in BOB resulted in Trichodesmium spp. bloom and a lower phytoplankton diversity and evenness in BOB, while the phytoplankton in the deep layer are senescent cells that sink down from the upper layer and cannot actively regulate the state of the cells, resulting in a higher sinking rate. Depth and temperature have a great impact on the physiological state of phytoplankton. The sinking rate of phytoplankton depend on the dominant groups composing the phytoplankton community. For the eastern Indian Ocean, seawater stratification caused by temperature changes the distribution of nutrients in the upper layer, and phytoplankton are affected by temperature and nutrients, resulting in changes in community structure, and finally showing different subsidence characteristics. [ABSTRACT FROM AUTHOR]

Ocean acidification (OA) and global warming-induced water column stratification can significantly alter phytoplankton-related biological activity in the marine ecosystem. Yet how these changes may play out in the tropical Indian Ocean remains unclear. This study investigated the ecological and metabolic responses of the different phytoplankton functional groups to elevated CO2 partial pressure and nitrate deficiency in two different environments of the eastern Indian Ocean (EIO). It is revealed that phytoplankton growth and metabolic rates are more sensitive to inorganic nutrients rather than CO2. The combined interactive effects of OA and N-limitation on phytoplankton populations are functional group-specific. In particular, the abundance and calcification rate of calcifying coccolithophores are expected to be enhanced in the future EIO. The underlying mechanisms for this enhancement may be ascribed to coccolithophore's lower carbon concentrating mechanisms (CCMs) efficiency and OA-induced [HCO3−] increase. In comparison, the abundance of non-calcifying microphytoplankton (e.g., diatoms and dinoflagellates) and primary productivity would be inhibited under those conditions. Different from previous laboratory experiments, interspecific competition for resources would be an important consideration in the natural phytoplankton populations. These combined factors would roughly determine calcifying coccolithophores as "winners" and non-calcifying microphytoplankton as "losers" in the future ocean scenario. Due to the large species-specific differences in phytoplankton sensitivity to OA, comprehensive investigations on oceanic phytoplankton communities are essential to precisely predict phytoplankton ecophysiological response to ocean acidification. [ABSTRACT FROM AUTHOR]

To examine the spatial pattern and controlling factors of the primary productivity (PP) of phytoplankton in the eastern Indian Ocean (EIO), deck-incubation carbon fixation (a 14C tracer technique) and the related hydrographic properties were measured at 15 locations during the pre-summer monsoon season (February–April 2017). There are knowledge gaps in the field observations of PP in the EIO. The estimated daily carbon production rates integrated over the photic zone ranged from 113 to 817 mgC m–2 d–1, with a mean of 522 mgC m–2 d–1. The mixed-layer integrated primary production (MLD-PP) ranged from 29.0 to 303.7 mgC m–2 d–1 (mean: 177.2 mgC m–2 d–1). The contribution of MLD-PP to the photic zone-integrated PP (PZI-PP) varied between 19 and 51% (mean: 36%). Strong spatial variability in the carbon fixation rates was found in the study region. Specifically, the surface primary production rates were relatively higher in the Bay of Bengal domain affected by riverine flux and lower in the equatorial domain owing to the presence of intermonsoonal Wyrtki jets, which were characterized by a depression of thermocline and nitracline. The PZI-PP exhibited a linear (positive) relationship with nutrient values, but with no significance, indicating a partial control of macronutrients and a light limitation of carbon fixation. As evident from the vertical profiles, the primary production process mainly occurred above the nitracline depth and at high photosynthetic efficiency. Phytoplankton (>5 μm), including dinoflagellates, Trichodesmium, coccolithophores, and dissolved nutrients, are thought to have been correlated with primary production during the study period. The measured on-deck biological data of our study allow for a general understanding of the trends in PP in the survey area of the EIO and can be incorporated into global primary production models.

Under the background of global warming, the area extent of the oligotrophic tropical oceans has growing due to increased water-column stratification over the past decades. Picophytoplankton is usually the most dominant phytoplankton group in oligotrophic tropical oceans and substantially contribute to carbon biomass and primary production three. Understanding how vertical stratification governs the community structure of picophytoplankton communities in oligotrophic tropical oceans is important for comprehensively understanding the plankton ecology and biogeochemical cycle in these areas. In this study, the distribution of the picophytoplankton communities in the eastern Indian Ocean (EIO) was investigated during a period of thermal stratification in the spring of 2021. Prochlorococcus contributed most (54.9%) to picophytoplankton carbon biomass, followed by picoeukaryotes (38.5%) and Synechococcus (6.6%). Vertically, the three picophytoplankton groups showed quite different distribution pattern: the abundance of Synechococcus was highest in the surface layer, while Prochlorococcus and picoeukaryotes were usually located between 50 m and 100 m. The relationship between the abundance of picophytoplankton and environmental factors was analyzed, and the results revealed that picophytoplankton distribution was strongly correlated with the degree of vertical stratification of the water column. The density of Synechococcus was higher in strongly stratified waters, while Prochlorococcus was more abundant in regions of weaker stratification. This is mainly attributed to variation of physicochemical parameters such as nutrient structures and temperature resulted from water column stratification. Understanding the distribution patterns of these organisms and their relationship with stratification in the oligotrophic EIO is essential for comprehensive understanding on oligotrophic tropical ecosystem with increasing stratification in future. • Picophytoplankton community in the eastern Indian Ocean is presented. • Prochlorococcus contributes more biomass to the open ocean than the Bay of Bengal. • Picophytoplankton distribution is correlated with the vertical stratification index. • Values of vertical stratification index increase with increasing latitude. [ABSTRACT FROM AUTHOR]

Abstract: On a seasonal cycle, the phytoplankton biomass off the North West Cape region of Australia surrounding Ningaloo Reef increased during the austral autumn and peaked in winter. In this study, historical field data sets, satellite-derived ocean color observations and output from a data-assimilating numerical ocean model are used to investigate physical mechanisms that influence the seasonal variability in phytoplankton biomass off this coast. The mixed layer depth (MLD) off Ningaloo deepened to ∼100m in autumn, partly due to the acceleration of the Leeuwin Current, which induced an increase in nutrient concentrations and is the key mechanism responsible for the seasonal enhancement in chlorophyll a concentrations observed in the waters off Ningaloo Reef. By comparing the MLDs and the critical depth it is suggested that while average irradiance throughout the MLD was low, this had less impact on net growth than grazing. [Copyright &y& Elsevier]

• A cruise was conducted along 88°E in the EIO during the intermonsoon period. • Phytoplankton growth and mortality rates were studied using dilution experiments. • Multiple phytoplankton blooms were observed along the meridional transect. • Island effect near the Maldives or a cyclonic eddy induced phytoplankton blooms. • EIO is not a stable oligotrophic marine ecosystem as affected by physical events. The Eastern Indian Ocean (EIO) is considered to exhibit low chlorophyll a (Chl a) concentrations. However, high Chl a levels were occasionally reported and attributed to physical events that frequently occur in this area. Because of limited in situ observations, the underlying formation mechanism of high Chl a through the responses of components within the ecosystem is not fully understood. In this study, we conducted a meridional cruise along the 88°E longitude in the EIO (16.5°N to 20°S) during the fall intermonsoon period (November 8–28th, 2018). For the first time, surface phytoplankton growth and mortality rates were measured using dilution experiments. Sensitive nutrient analysis was also applied to determine the nanomolar nutrient concentrations. Our cruise captured high surface Chl a concentrations (∼298 ng L−1 at 10 m) in the Bay of Bengal, at the Equator, and in the southern EIO, even under consistent nitrogen depletion (dissolved inorganic nitrogen of ∼58 nM). All the blooms observed were in the decay phase, as suggested by the satellite observations of Chl a distributions and the low or negative phytoplankton net growth rate. However, the phytoplankton growth rate was enhanced by ∼4.9 times due to nutrient enrichment in dilution experiments, indicating that the bloom was initiated by nutrient enrichment and ceased by the severe nutrient limitation. The shipboard acoustic Doppler current profiler measurements and satellite observations of sea surface winds, surface currents, and sea surface height collectively suggested that phytoplankton blooms were triggered by nutrient enrichments that were possibly supplied by either the island effect near the Maldives or a cyclonic eddy in the southern EIO. In the former case, the Chl a -rich water generated near the islands was then advected eastward toward the study area by surface currents associated with the Wyrtki Jet. Our study indicated that the EIO was not a stable oligotrophic marine ecosystem with consistently low Chl a concentrations, and was quite dynamic as affected by unique regional physical events. [ABSTRACT FROM AUTHOR]

[Display omitted] • Atmospheric deposition and upwelling increase nitrogen budget of tropical seas. • Nitrogen inputs enhanced carbon flux through promoting primary productivity. • The competition for nutrients caused the regime shift from cyanobacteria to diatom. • The C/N ratio would alter along with changed nutrient status and community structure. Exogenous nutrient inputs increasingly impact the structure and function of marine food weds through abiotic and biotic ways. The influence of nutrient inputs from atmospheric depositions and intermediate seawater supply on the phytoplankton growth and stoichiometry was studied in the Eastern Indian Ocean through multifactorial mesocosm experiments. Our results indicate that nitrogen inputs from atmospheric deposition and hydrodynamic processes did not only promote chlorophyll a biomass (5.3–58.52 μg·L-1) to increase the photosynthetically fixed carbon content but also generate a regime shift from picophytoplankton cyanobacteria to microphytoplankton diatom indicated by high relative abundance of microphytoplankton (>50%). These alternations of nutrient status and phytoplankton community structure reduced biodiversity of marine ecosystems (N-cultures, 0.18 to 0.56; control, 0.63) and generated the attendant C/N ratio of 5.6 ± 0.2 in cultures with nitrogen addition that was significantly lower than cultures with only phosphorus or iron addition. These results provide a thorough insight about the influence of nutrient inputs on marine biogeochemical cycle. Considering the universality of phytoplankton community alteration induced by exogenous nutrient inputs, integrating community compositions with phytoplankton stoichiometry may be helpful for clarify mechanisms of environment events impacting marine food weds. [ABSTRACT FROM AUTHOR]