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The present study investigated whether there is a critical length of photoperiod needed to stimulate a completed parr–smolt transformation (PST) in Atlantic salmon Salmo salar. In two experiments, S. salar parr of the Norwegian aquaculture strain held on continuous light were exposed to a short photoperiod (6 L:18D) followed by exposure to 8 L:16D, 12 L:12D, 16 L:8D, 20 L:4D and 24 L:0D in experiment 1 or to 6 L:18D followed by maintenance on 6 L:18D or exposure to 12 L:12D and 24 L:0D photoperiods in experiment 2. All groups, irrespective of photoperiod treatment, developed improved hypo‐osmoregulatory ability. However, the development was greatest in the groups exposed to 20 L:4D and 24 L:0D in experiment 1 and 24 L:0D in experiment 2. In experiment 2, gill Na+– K+‐ATPase activity increased in the group exposed to 24 L:0D, but not in the groups exposed to 12 L:12D and 6 L:18D. The groups exposed to 20 L:4D and 24 L:0D in experiment 1 and 24 L:0D in experiment 2 also grew better than fish exposed to shorter photoperiods. In experiment 2 only the group exposed to 24 L:0D showed a decrease in condition factor and increases in plasma growth hormone and brain type 2 deiodinase mRNA abundance. Hence, only the groups exposed to photoperiods above 16 L:8D developed classical smolt indices in the present experiment, leading us to conclude that the photoperiod increase needs to exceed 16 h daylight for stimulating a complete PST in the S. salar used in the present study. [ABSTRACT FROM AUTHOR]

This paper reports a study of the activity of calcium-dependent proteases (calpains) and proteasome in the skeletal muscles of parrs and smolts of the Atlantic salmon Salmo salar L. from the Indera River (the Kola Peninsula). It was demonstrated that the growth rate and protein mass accumulation during smoltification in salmonid fishes are inversely related to the activity of proteases in their muscles. It was noted that decreased protein degradation in the skeletal muscles of salmon juveniles serves as a mechanism ensuring the intensive growth and physiological maturity necessary for migration from the river to the sea. [ABSTRACT FROM AUTHOR]

Runoff of metals represents one of the major environmental challenges related to historic and ongoing mining activity. In this study, transcriptomics (direct RNA sequencing [RNA-seq] and reverse-transcription quantitative polymerase chain reaction [RT-qPCR]) was used to predict toxicity of metal-rich acid mine drainage (AMD) water collected in the abandoned copper (Cu) mine called Løkken Mine on Atlantic salmon liver and kidney, the main target organs of Cu-induced toxicity in fish. Smolts were exposed to control and diluted AMD water, which contains a mixture of metals but is especially enriched with Cu, at 4 concentrations in freshwater (FW) for 96 h, and then were transferred to and kept in seawater (SW) for another 24 h. Significant accumulation of Cu was observed in the gills, but not liver and kidney tissues, after 96 h of exposure. Short-term exposure to metal-rich ADM (high exposure group) significantly upregulated 3201 transcripts and downregulated 3782 transcripts in liver. The strongest effect attributed to exposure was observed on the KEGG pathway “protein processing in endoplasmic reticulum,” followed by “steroid biosynthesis.” Gene ontology (GO) analysis suggested that exposure predominantly affected “protein folding,” possibly by disrupting disulfide bonds as a result of endoplasmic-reticulum-generated stress, and “sterol biosynthetic processes.” Transfer to uncontaminated SW for 24 h amended the transcription of several genes, suggesting a transient effect of treatment on some mechanisms. In conclusion, the data show that trace metals in AMD from abandoned pyrite mines might disturb molecular mechanisms linked to protein folding in Atlantic salmon smolt endoplasmic reticulum. [ABSTRACT FROM PUBLISHER]

We examined Hsc70 in gill, liver and caudal fin from coho salmon ( Oncorhynchus kisutch) before (ambient, ~12 °C) and after a sublethal heat shock of 25 °C for 1 h. Increased levels were observed for at least 48 h in all three tissues. Attempts to demonstrate isoforms of this heat-shock protein were not successful using four different antibodies. However, one of these antibodies recognized isoforms in brine shrimp and oysters, two organisms in which the heat-shock response has been well characterized. Extracts of those organisms and coho salmon tissues were run on the same gels and evaluated on the same Western blots, at the same time. We believe that our results provide a reliable account of Hsc70 in these fish under these conditions. Limited experiments showed that induced thermotolerance was achieved in these coho salmon, lasting for about 1 week after the sublethal heat shock. These data suggest that increased levels of Hsc70 are correlated with induced thermotolerance in these fish. [ABSTRACT FROM AUTHOR]

When odorants bind to the sensory cilia of olfactory sensory neurons, the cells respond with an electrical output signal, typically a short train of action potentials. This review describes the present state of knowledge about the olfactory signal transduction process. In the last decade, a set of transduction molecules has been identified which help to explain many aspects of the sensory response. Odor-induced second-messenger production, activation of transduction channels, the central role of the ciliary Ca2+ concentration, as well as mechanisms that mediate adaptation, are all qualitatively understood on the basis of a consistent scheme for chemoelectrical transduction. This scheme, although necessarily incomplete, can serve as a working model for further experimentation which may reveal kinetical aspects of signal transduction processes in olfactory sensory neurons. [ABSTRACT FROM AUTHOR]

Superoxide (O2 ) poses multiple threats, which are diminished by a family of metalloenzymes, the superoxide dismutases. Among the damaging effects of O are direct oxidation of low-molecular2 weight reductants; inactivation of a select group of enzymes; and reaction with NO to yield the strong oxidant, peroxynitrite. Of even greater import is the ability of O to univalently oxidize the [4 Fe2 4 S] clusters of dehydratases, which causes release of iron. The "free" iron, which is kept reduced by cellular reductants, then reduces hydroperoxides to hydroxyl or alkoxyl radicals. Because the "free" iron will preferentially bind to anionic polymers, such as nucleic acids, or to anionic surfaces, such as cell membranes, these radicals will be generated adjacent to these vital targets and will preferentially attack them. O and iron can thus be viewed as part2 ners in crime, and reciprocal regulatory effects between iron and O2 may be anticipated. These are discussed. [ABSTRACT FROM AUTHOR]

Presents an overview of the different classes of metalloregulatory proteins whose activities are liable to be modulated in response to nitric oxide (NO) production in various species. Synthesis and reactivity of NO; Metal ion-responsive DNA-binding proteins; DNA-binding proteins responsive to or containing heme; Regulatory proteins containing zinc.